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Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera

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Abstract

The effects of yeast extract and selected polysaccharide elicitors on secondary metabolite production, particularly of anthocyanin and phenolic acid, in cell suspension cultures of Vitis vinifera were investigated. All elicitors either maintained or promoted cell growth in culture. Overall, secondary metabolite production in V. vinifera cell suspension cultures responded differently to different elicitors. Chitosan, pectin, and alginate enhanced production of anthocyanin within 13 days of culture with levels of 2.5-, 2.5-, and 2.6-fold increase, respectively, over that of control. Chitosan, alginate, and gum arabic significantly promoted accumulation of phenolic acids, particularly 3-O-glucosyl-resveratrol, in V. vinifera cultures, as well as in the culture medium. Intracellular phenolic acid production was significantly enhanced by alginate and chitosan, with 1.7- and 1.5-fold levels, respectively, of that of control. Extracellular phenolic acid production was also significantly increased in the presence of chitosan and gum arabic, with levels of 3.3- and 1.7-fold higher, respectively, than those of control. In addition, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity was enhanced in the presence of elicitors, and this was positively correlated with increased accumulation of anthocyanin in V. vinifera cell suspension cultures.

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Abbreviations

DPPH:

1,1-Diphenyl-2-picrylhydrazyl

DW:

Dry weight

FW:

Fresh weight

HPLC:

High performance liquid chromatography

LC–MS:

Liquid chromatography–mass spectrometry

NMR:

Nuclear magnetic resonance

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Acknowledgments

The authors are grateful to Irene Hemmerich and Nay Min Min Thaw Saw from Berlin University of Technology for continuous cultivation of V. vinifera cell culture and technical assistance. The authors also thank Daniel Engelhart from the Fritz Haber Institute for help with linguistic questions.

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Authors and Affiliations

  1. Institute of Food Biotechnology and Food Chemistry, Berlin University of Technology, Königin-Luise Str. 22, 14195, Berlin, Germany

    Zhenzhen Cai, Anja Kastell, Dietrich Knorr & Iryna Smetanska

  2. Leibniz Institute of Vegetable and Ornamental Crops Grossbeeren and Erfurt e.V., Theodor-Echtermeyer-Weg 1, 14979, Grossbeeren, Germany

    Inga Mewis

  3. Department of Plant Food Processing, University of Applied Science Weihenstephan-Triesdorf, Steingruber Str. 2, 91746, Weidenbach, Germany

    Iryna Smetanska

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  1. Zhenzhen Cai
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  2. Anja Kastell
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Correspondence to Zhenzhen Cai.

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Cai, Z., Kastell, A., Mewis, I. et al. Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera . Plant Cell Tiss Organ Cult 108, 401–409 (2012). https://doi.org/10.1007/s11240-011-0051-3

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  • DOI: https://doi.org/10.1007/s11240-011-0051-3

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