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In vitro photoautotrophic potential and ex vitro photosynthetic competence of Pfaffia glomerata (Spreng.) Pedersen accessions

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Abstract

This study aimed to investigate whether the in vitro photoautotrophic potential and 20 hydroxyecdysone (20E) production of six Pfaffia glomerata accessions could be correlated to photosynthetic performance and biomass accumulation under ex vitro conditions in a greenhouse environment. Our results showed that P. glomerata accessions displayed varying photosynthetic rates that may have resulted in differential biomass accumulation both in vitro and ex vitro. Accessions A4, A13 and A43 showed the highest dry weight under in vitro photoautotrophic conditions, coinciding with the higher photosynthetic rates of the same accessions in greenhouse. They also showed the highest 20E mass per plant ex vitro. Production of 20E in vitro also varied among accessions, with the highest total mass achieved by accessions A4 and A43. In addition to having the potential for optimizing propagation by reducing the duration of the in vitro culture stage and lessening death rate during acclimatization, the use of these genotypes with higher in vitro photoautotrophic potential can guarantee highly productive plants ex vitro because the biomass accumulation pattern observed in vitro matched that under greenhouse conditions.

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Acknowledgments

The authors thank the National Council for Scientific and Technological Development (CNPq) [MCT 480675/2009-0; PDJ 500874/2012-3; PQ 303201/2010-10 and MCTI 459529/2014-5 to WCO], the Minas Gerais State Research Foundation (FAPEMIG) [CAG-APQ-01036-09; CRA-APQ-01651-13; CRA-BPD-00046-14], and CAPES (PNPD) for financial support. We also thank Dr Roberto Vieira and Dr Rosa das Neves Alves (National Center for Genetic Resources and Biotechnology - Embrapa/Cenargen, Brasília, DF, Brazil) for providing P. glomerata accessions.

Conflict of interests

The authors declare that there are no conflicts of interests.

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Authors and Affiliations

  1. Laboratório de Cultura de Tecidos (LCTII)/BIOAGRO, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Campus Universitário, Avenida Peter Henry Rolfs s/n, Viçosa, MG, 36570-900, Brazil

    João Paulo Oliveira Corrêa, Marcos Vinícius Marques Pinheiro, Diego Silva Batista, Jéssica Fernanda Laísca Azevedo, Ana Cláudia Ferreira da Cruz & Wagner Campos Otoni

  2. Laboratório de Fisiologia Molecular de Plantas, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil

    Camilo Elber Vital

  3. Centro de Pesquisas em Florestas, Fundação Estadual de Pesquisa Agropecuária, BR 287, Acesso VCR 830, km 4,5, Boca do Monte, CP 346, Santa Maria, RS, 97001-970, Brazil

    Cleber Witt Saldanha

  4. Laboratório de Nutrição e Metabolismo de Plantas, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil

    Fábio Murilo DaMatta

Authors
  1. João Paulo Oliveira Corrêa
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  2. Camilo Elber Vital
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  3. Marcos Vinícius Marques Pinheiro
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  4. Diego Silva Batista
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  5. Jéssica Fernanda Laísca Azevedo
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  6. Cleber Witt Saldanha
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  7. Ana Cláudia Ferreira da Cruz
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  8. Fábio Murilo DaMatta
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  9. Wagner Campos Otoni
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Correspondence to Wagner Campos Otoni.

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Corrêa, J.P.O., Vital, C.E., Pinheiro, M.V.M. et al. In vitro photoautotrophic potential and ex vitro photosynthetic competence of Pfaffia glomerata (Spreng.) Pedersen accessions. Plant Cell Tiss Organ Cult 121, 289–300 (2015). https://doi.org/10.1007/s11240-014-0700-4

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  • DOI: https://doi.org/10.1007/s11240-014-0700-4

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