Skip to main content
SpringerLink
Log in

Anthelmintic effects of Salix babylonica L. and Leucaena leucocephala Lam. extracts in growing lambs

  • Regular Articles
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Twenty Katahdin × Pelibuey crossbreed male lambs, 3 to 4 months of age and 24 ± 0.3 kg of body weight, were used to study the anthelmintic effects of administering extracts of Salix babylonica L. (SB) and Leucaena leucocephala Lam. (LL). Lambs had not been treated with anthelmintics previously and were randomly allocated into four groups of five lambs each in a completely randomized design. Treatments were as follows: control (lambs fed on total mixed ration without extracts), SB (as control plus S. babylonica L. extract at 30 ml/day), LL (as control plus L. leucocephala Lam. extract at 30 ml/day), and SBLL (as control plus 30 ml/day of S. babylonica L. and L. leucocephala Lam. extracts in a 1:1 (v/v) mixture) for 63 days. Extracts were orally administered before the 8:00 a.m. feeding to each lamb. Rectal fecal samples were collected from each lamb at day 22 (P1), day 43 (P2), and day 63 (P3) of the experiment. Adult worm and egg counts were determined in each fecal sample immediately after collection. Plant secondary metabolites of total phenolics, saponins, and the aqueous fraction were 50 % lower in the SB versus LL extracts. Overall, the oral administration of extracts has improved the egg and worm count reductions in lamb feces by 54, 47, and 40 % for LL, SB, and SBLL, respectively, versus the control lambs. Reductions of worm egg counts in lamb feces were higher (P < 0.05) in P2 and P3 versus P1. Extracts of SB and LL or possibly isolated bioactive compounds could be a promising alternative to conventional anthelmintics to treat gastrointestinal parasites of small ruminants. Such treatments could be used in control strategies against gastrointestinal nematodes in organic and conventional production systems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • AOAC, Association of Official Analytical Chemists, 1997. Official Methods of Analysis, 16th ed. AOAC, Arlington, VA, USA.

    Google Scholar 

  • Canul-Ku, H.L., Rodríguez-Vivas, R.I., Torres-Acosta, J.F.J., Aguilar-Caballero, A.J., Pérez-Cogollo, L.C. and Ojeda-Chi, M.M., 2012. Prevalence of cattle herds with ivermectin resistant nematodes in the hot sub-humid tropics of Mexico. Veterinary Parasitology, 183, 292–298.

    Article  CAS  PubMed  Google Scholar 

  • Carvalho, C.O., Chagas, A.S., Cotinguiba, F., M. Furlan, Brito, L.G., Chaves, F.C.M., Stephan, M. P., Bizzo, H.R. and Amarante, A.F.T., 2012. The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis. Veterinary Parasitology, 183, 260–268.

    Article  PubMed  Google Scholar 

  • Cowan, M.M., 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12, 564–582.

    CAS  PubMed Central  PubMed  Google Scholar 

  • Desta, B., 1995. Ethiopian traditional herbal drugs. Part I. Studies on the toxicity and therapeutic activity of local taenicidal medications. Journal of Ethnopharmacology, 45, 27–33.

    Article  CAS  PubMed  Google Scholar 

  • El-Kamali, H.H. and El-Khalifa, K.F., 1999. Folk medicinal plants of riverside forests of the Southern Blue Nile district, Sudan. Fitoterap,70, 493–497.

    Article  Google Scholar 

  • Engel, C., 2007. Zoopharmacology In: Wynn S.G, and Fougere (Eds). Veterinary Herbal medicine. (pp7-15). Library of Congress Cataloging-in Publication Data. 695p.

  • Francis, G., Kerem, Z., Makkar, H.P. and Becker, K., 2002. The biological action of saponins in animal systems: a review. British journal of Nutrition, 88, 587–605.

    Article  CAS  PubMed  Google Scholar 

  • Hoste, H., Jackson, F., Athanasiadou, S., Thamsborg, S.M. and Hoskin, S.O. 2006. The effects of tannin-rich plants on parasitic nematodes in ruminants. Trends in Parasitology, 22(6), 253–261.

    Article  CAS  PubMed  Google Scholar 

  • Kabore A., Traore A., Nignan M., Gnanda B.I., Valentin, B., Tamboura, H., Bele, M.A. and Gaston, M., 2012. In vitro anthelmintic activity of Leuceana leucocephala (Lam.) De Wit. (Mimosaceae) and Gliricidia sepium (Jacq.) Kunth ex Steud (Fabaceae) leave extracts on Haemonchus contortus ova and larvae. Journal of Chemical and Pharmaceutical Research, 4(1), 303-309.

    Google Scholar 

  • Littell, R.C., Henry, P.R. and Ammerman, C.B., 1998. Statistical analysis of repeated measures data using SAS procedures. Journal of Animal Science, 76, 1216–1231.

    CAS  PubMed  Google Scholar 

  • MAFF, 1979. Parasitological laboratory techniques, Tech. Bull., No. 18. Ministry of agriculture fisheries and food manual of veterinary. Her Majestey’s Stationary Office, London.

  • Méndez-Ortíz, F.A., Sandoval-Castro, C.A. and Torres-Acosta, J.F.J., 2012. Short term consumption of Havardia albicans tannin rich fodder by sheep: Effect on feed intake, diet digestibility and excretion of Haemonchus contortus eggs. Animal Feed Science and Technology, 176, 185–191.

    Article  Google Scholar 

  • NRC, 1985. Nutrient Requirements of Domestic Animals. National Research Council, Washington, DC, USA.

    Google Scholar 

  • Ojeda-Robertos, N.F., Torres-Acosta, J.F.J., Ayala-Burgos, A., Aguilar-Caballero, A.J., Cob-Galera, L.A. and Mendoza–de-Gives, P., 2008. A technique for the quantification of Duddingtonia flagrans chlamydospores in sheep faeces. Veterinary Parasitology, 152, 339–343.

    Google Scholar 

  • Ríos-de Álvarez, L., Jackson, F., Greer, A., Bartley, Y., Bartley, D.J., Grant, G. and Huntley, J.F., 2012. In vitro screening of plant lectins and tropical plant extracts for anthelmintic properties. Veterinary Parasitology, 186(3–4), 390–8.

    Article  PubMed  Google Scholar 

  • Salem, A.Z.M., Olivares, M., López, S., González-Ronquillo, M., Rojo, R., Camacho, L.M., Cerrillo, S.M.A. and Mejia, H.P., 2011a. Effect of natural extracts of Salix babylonica and Leucaena leucocephala on nutrient digestibility and growth performance of lambs. Animal Feed Science and Technology, 170, 27–34.

    Article  Google Scholar 

  • Salem, A.Z.M., Salem, M.Z.M., González-Ronquillo, M., Camacho, L.M. and Cipriano, M., 2011b. Major chemical constituents of Leucaena leucocephala and Salix babylonica leaf extracts. Journal of Tropical Agriculture 49 (1–2), 95–98.

    CAS  Google Scholar 

  • SAS, 2002. SAS User´s Guide: Statistics. Ver 9.0. SAS Institute. Cary, N.C. USA. 956 p.

  • Satou, T., Koga, M., Matsuhashi, R., Koike, K., Tada, I. and Nikaido, T., 2002. Assay of nematocidal activity of isoquinoline alkaloids using third-stage larvae of Strongyloides ratti and S. venezuelensis. Veterinary Parasitology, 104, 131–138.

    Article  CAS  PubMed  Google Scholar 

  • Schoenian, S., 2008. Understanding anthelmintics (dewormers). Small Ruminant Info. Series. Western Maryland Research and Education Center, University of Maryland cooperative extension.

  • Tarnopolsky, M.A. and Beal, M.F., 2001. Potential for creatine and other therapies targeting cellular energy dysfunction in neurological disorders. Annals of Neurology, 49, 561–574.

    Article  CAS  PubMed  Google Scholar 

  • Terrill, T.H., Larsen, M., Samples, O.M., Husted, S., Miller, J.E., Kaplan, R.M. and Gelaye, S., 2004. Capability of the nematodetrapping fungus Duddingtonia flagrans to reduce infective larvae of gastrointestinal nematodes in goat feces in the southeastern United States: dose titration and dose time interval studies. Veterinary Parasitology, 120, 285–296.

    Article  CAS  PubMed  Google Scholar 

  • Thompson, D.P. and Geary, T.G., 1995. The structure and function of helminth surfaces. In: Marr, J.J., Muller, M. (Eds.), Biochemistry and Molecular Biology of Parasites, 1st Edition. Academic Press, NewYork, pp. 203–232.

    Chapter  Google Scholar 

  • Van Soest, P.J., Robertson, J.B. and Lewis, B.A., 1991. Methods of dietary fiber, neutral detergent fiber and non-polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583–3597.

    Article  PubMed  Google Scholar 

  • Wabo-Pone, J., Mbida, M. and Bilong-Bilong C.F., 2009. In vivo evaluation of potential nematicidal properties of ethanol extract of Canthium mannii (Rubiaceae) on Heligmosomoides polygyrus parasite of rodents. Veterinary Parasitology, 166, 103–107.

    Article  CAS  PubMed  Google Scholar 

  • Wharton, D.A., 1986. Life cycle. In: Calow, P. (Ed.), A Functional Biology of Nematodes. Croom Helm, London, pp. 118–148.

    Chapter  Google Scholar 

  • Wasswa, P. and Olila, D., 2006. The in vitro Ascaricidal activity of selected indigenous medicinal plants used in ethno veterinary practices in Uganda. African Journal of Traditional, Complementary and Alternative medicines, 3, 94–103.

    Google Scholar 

  • Wynn S.G. and Fougere B J., 2007. Introduction: Why use herbal medicine. In: Wynn S.G, and Fougere (Ed). Veterinary Herbal medicine. Library of Congress cataloging-in publication data. p 695.

Download references

Acknowledgments

The authors wish to acknowledge the financial support from la SEP, Mexico, Project PROMEP 103.5/09/4195.

Author information

Authors and Affiliations

  1. Faculty of Veterinary and Livestock, Autonomous University of Guerrero, Altamirano, P.O. 40660, Mexico

    Pablo Mejia Hernandez, Moisés Cipriano-Salazar, Blas Cruz-Lagunas & Luis Miguel Camacho

  2. Faculty of Veterinary and Livestock, Autonomous University of the Mexico State, Toluca, P.O. 50000, Mexico

    Abdelfattah Z. M. Salem & Mona M. M. Y. Elghandour

  3. Faculty of Agriculture El-Shatby, Alexandria University, Alexandria, Egypt

    Abdelfattah Z. M. Salem

Authors
  1. Pablo Mejia Hernandez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Abdelfattah Z. M. Salem
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mona M. M. Y. Elghandour
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Moisés Cipriano-Salazar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Blas Cruz-Lagunas
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Luis Miguel Camacho
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Abdelfattah Z. M. Salem or Luis Miguel Camacho.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hernandez, P.M., Salem, A.Z.M., Elghandour, M.M.M.Y. et al. Anthelmintic effects of Salix babylonica L. and Leucaena leucocephala Lam. extracts in growing lambs. Trop Anim Health Prod 46, 173–178 (2014). https://doi.org/10.1007/s11250-013-0471-7

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-013-0471-7

Keywords

Search

Navigation