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Acaricide resistance in Rhipicephalus microplus collected from selected districts of Madhya Pradesh, Uttar Pradesh and Punjab states of India

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Abstract

The isolates of Rhipicephalus microplus collected from Madhya Pradesh (MP), Punjab (PJB) and Uttar Pradesh (UP) states of India were characterized using laboratory standardized adult immersion test (AIT) against macrocyclic lactone (ivermectin), synthetic pyrethroids (cypermethrin and deltamethrin), organophosphates (coumaphos and diazinon) and phenylpyrazole compounds (fipronil). Out of the six isolates tested, five isolates except MTH were resistant to deltamethrin and cypermethrin at level II with RF ranging from 16.4 to 24.02 and 7.05 to 13.2, respectively. In case of organophosphates, coumaphos was less effective showing resistance level II (RF 8.52–11.2) in all the six populations compared with diazinon to which three isolates (MHW, RWA and AGS) were resistant at level II. Except MTH, other five isolates were categorized at level I with RF ranging from 1.53 to 3.02 against ivermectin. The phenylpyrazole compound however was found effective, and none of the isolates could survive at a discriminating concentration. The possible strategy for the management of multi-acaricide-resistant ticks in the surveyed districts was discussed in the present study.

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Funding

The authors received funding from the Indian Council of Agricultural Research, New Delhi, through the National Agricultural Science Fund (Grant number NASF/ABA-6015/2016-17/357 and NFBDFARA/BSA-4004/2013-14) and Department of Science and Technology, New Delhi (DST-SERB-PDF/2016/001816).

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  1. Rinesh Kumar & Suman Kumar

    Present address: Department of Parasitology, College of Veterinary Science and Animal Husbandry, Rewa, Madhya Pradesh, India

Authors and Affiliations

  1. Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

    Sharath V. Sagar, Kuldeep Saini, Anil Kumar Sharma, Sachin Kumar, Rinesh Kumar, Ashutosh Fular, Mukesh Shakya, Deepak Upadhaya, Gaurav Nagar, Shanmuganath C., Subhamoy Samanta, Suman Kumar & Srikant Ghosh

  2. Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Mhow, Madhya Pradesh, 453446, India

    Mukesh Shakya

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Correspondence to Srikant Ghosh.

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Necessary permission to use large animals for tick feeding was taken from the regulatory authority: Committee for the Purpose of Control and Supervision of Experimentation on Animals (CPCSEA).

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Sagar, S.V., Saini, K., Sharma, A.K. et al. Acaricide resistance in Rhipicephalus microplus collected from selected districts of Madhya Pradesh, Uttar Pradesh and Punjab states of India. Trop Anim Health Prod 52, 611–618 (2020). https://doi.org/10.1007/s11250-019-02048-0

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