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Effect of dietary supplementation of Bacillus subtilis spores on growth performance, oxidative status, and digestive enzyme activities in Japanese quail birds

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Abstract

The present trial investigated the feeding effect of B. subtilis spores on growth performance, blood metabolites, antioxidative status, and digestive enzyme activities in growing quails. A total of 750 1-day-old Japanese quail chicks were randomly allotted equally into five experimental groups: control (BS0) fed a maize-soybean basal diet with no additives, the others were supplemented with: B. subtilis spores with the levels of 1 × 103 (BS3), 1 × 105 (BS5), 1 × 107 (BS7), and 1 × 109 (BS9)/kg diet. Quails fed on B. subtilis diets exhibited linearly increasing live body weight and body weight gain and decreased feed-to-gain ratio compared with the control group. Daily feed intake was not significantly altered. Increasing levels of B. subtilis led to a linear increase in serum total protein and albumin levels, and a linear decrease in concentrations of glucose, creatinine, urea-N, aspartate aminotransferase, and alanine aminotransferase. Hypolipidemic impact of feeding B. subtilis spores was greatly observed and enhanced by increasing its dietary inclusion level. Triiodothyronine and thyroxine activities were significantly elevated in treated groups. Glutathione content and catalase activities were linearly increased in groups BS7, BS9, and BS5, while lipid peroxidation was decreased in all treatment groups. Duodenal proteolytic, lipolytic, and amylolytic activities as well as nutrient digestibility were linearly increased in treated groups. In conclusion, dietary supplementation of B. subtilis spores almost at all studied levels was able to promote the antioxidative status and digestive enzymes activities, while only the high concentrations (BS7 and BS9) could improve the nutrient digestion and growth performance of growing Japanese quail.

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Acknowledgments

The authors acknowledge the Biological Application Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Poultry and Fish Production Dept., Faculty of Agriculture, Menoufia University, Egypt, and Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt, for their cooperation.

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Authors and Affiliations

  1. Biological Application Department, Nuclear Research Center, Atomic Energy Authority, Abou-Zabael, 13759, Egypt

    Abdel-Moneim Eid Abdel-Moneim, Hamdy A. Basuony & Essam M. Sabic

  2. Poultry and Fish Production Department, Faculty of Agriculture, Menoufia University, Shibin El-Kom, Egypt

    Dina A. Selim

  3. Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, Kafr Elsheikh, Egypt

    Ahmed A. Saleh & Tarek A. Ebeid

  4. Department of Animal Production and Breeding, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, 51452, Saudi Arabia

    Tarek A. Ebeid

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  1. Abdel-Moneim Eid Abdel-Moneim
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  2. Dina A. Selim
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  4. Essam M. Sabic
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Correspondence to Abdel-Moneim Eid Abdel-Moneim.

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All the procedures used in this trial were approved by Local Experimental Animals Care Committee, and approved by the Institutional Ethics Committee.

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Abdel-Moneim, AM.E., Selim, D.A., Basuony, H.A. et al. Effect of dietary supplementation of Bacillus subtilis spores on growth performance, oxidative status, and digestive enzyme activities in Japanese quail birds. Trop Anim Health Prod 52, 671–680 (2020). https://doi.org/10.1007/s11250-019-02055-1

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