Abstract
This study was conducted to study the effect of protease as well as three dietary crude protein levels on growth and nutrient digestibility in broilers. The broiler chickens (n = 540) were assigned to six dietary treatments with six replicates. Three dietary crude protein (CP) levels (17, 19, and 21%) along with two levels of protease (0 and 30,000 IU/kg) were investigated in 3 × 2 factorial arrangement during the starter phase. Body weight was higher (P < 0.05) in group fed with CP-21; however, feed intake was significantly (P < 0.05) lower in CP-19 and CP-21. Resultantly, FCR was significantly (P < 0.05) improved in birds fed with CP-21. Similarly, body weight, feed intake, and FCR were significantly (P < 0.05) improved in enzyme-treated birds compared to the untreated birds. For practical purpose, birds fed CP-19 with protease enzyme resulted in improved FCR compared to birds fed with CP-21. The results revealed that CP and apparent metabolizable energy (AME) were significantly (P < 0.05) higher in birds fed with CP-21 along with supplementation of enzyme while abdominal fat was significantly (P < 0.05) lower in the same group compared to birds fed with CP-17. These results indicated that a diet having 21% CP supplemented with 30,000 IU/kg protease enzyme was superior in improving the performance and digestibility of nutrients in broiler during the starter phase.
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References
Abd El-Hack, M., Alagawany, M., Arif, M., Emam, M., Saeed, M., Arain, M.A., Siyal, F.A., Patra, A., Elnesr, S.S. and Khan, R.U., 2018. The uses of microbial phytase as a feed additive in poultry nutrition – a review. Annals of Animal Science 18, 639-658.
Alam, S., Masood, S., Zaneb, H., Rabbani, I., R.U. Khan, Shah, M., Ashraf, S. and Alhidary, I.A., 2019. Effect of Bacillus cereus and Phytase on the Expression of Musculoskeletal Strength and Gut Health in Japanese Quail (Coturnix japonica). Poultry Science Journal (in press)
Angel, C.R., Saylor, W., Vieira, S.L., Ward, N., 2011. Effects of a monocomponent protease on performance and protein utilization in 7-to 22-day-old broiler chickens. Poultry Science 90, 2281-2286.
Awad, E.A., Zulkifli, I., Soleimani, A.F., Aljuobori, A., 2017. Effects of feeding male and female broiler chickens on low-protein diets fortified with different dietary glycine levels under the hot and humid tropical climate. Italian Journal of Animal Science 16, 453–461
Berres, J., Vieira, S. L., Dozier III, W.A., Cortês, M. E. M., de Barros, R., Nogueira, E. T., and Kutschenko, M., 2010. Broiler responses to reduced-protein diets supplemented with valine, isoleucine, glycine, and glutamic acid. Journal of Applied Poultry Research, 19, 68–79.
Cowieson, A.J., Roos, F.F., 2016. Toward optimal value creation through the application of exogenous mono-component protease in the diets of nonruminants. Animal Feed Science and Technology. 1;221:331–40.
Doskovic, V., Bogosavljevic-boskovic, S., Pavlovski, Z., Miloševic, B., Škrbic, Z., Rakonjac, S. and Petricevic, V., 2013. Enzymes in broiler diets with special reference to protease. World’s Poultry Science Journal, 69, 343-360.
Erdaw, M. M., Wu, S., and Iji, P. A., 2017. Growth and physiological responses of broiler chickens to diets containing raw, full-fat soybean and supplemented with a high-impact microbial protease. Asian-Australasian Journal of Animal Science, 30,1303–1313.
Favero, A., Maiorka, A., Rocha, C., Appelt, M.D., Sorbara, J.O.B., 2009. Effect of protease enzyme on performance and ileal digestibility of broilers grown to 42 days of age in floor pens. International Poultry Scientific Forum, Atlanta, Georgia, Abstracts of Papers M28, pp. 9.
Fidelis F.N., Kluenter, A.M., Fischer, M. and Pontoppidan, K. 2010. A feed serine protease improves broiler performance and increases protein and energy digestibility. Poultry Science, 48,239-246.
Freitas, D.M., Vieira, S.L., Angel, C.R., Favero, A. and Maiorka, A. 2011. Performance and nutrient utilization of broilers fed diets supplemented with a novel mono-component protease. Journal of Applied Poultry Research, 20,322-334.
Fru-nji, F., Kluenter, A.M., Fischer, M. and Pontoppidan, K. 2011. A feed serine protease improves broiler performance and energy digestibility. Poultry Science 48, 239-246.
Ghazi, S., Rooke, J.A., Galbraith, H. and Bedford, M. R., 2002. The potential for the improvement of the nutritive value of soya-bean meal by different proteases in broiler chicks and broiler cockerels. British Poultry Science, 43,70–77.
Hafeez, A., Shah, S.A.A., R.U. Khan, Ullah, Q. and Naz, S., 2020. Effect of diet supplemented with phytogenics and protease enzyme on performance, serum biochemistry and muscle histomorphology in broilers. Journal of Applied Animal Research 48, 326-330: https://doi.org/10.1080/09712119.2020.1789648
Laudadio, V., Passantino, L., Perillo, A., Lopresti, G., Passantino, A., Khan, R.U. and Tufarelli, V., 2012. Productive performance and histological features of intestinal mucosa of broiler chickens fed different dietary protein levels. Poultry Science, 91(1), 265-270.
Law, F.L., Zulkifli, I., Soleimani, A.F., Liang, J.B. and Awad, E.A., 2018. The effects of low-protein diets and protease supplementation on broiler chickens in a hot and humid tropical environment. Asian-Australasian journal of animal sciences. (8):1291-1300.
Law, F.L., Idrus, Z., Soleimani Farjam, A., Juan Boo, L. and Awad, E.A., 2019. Effects of protease supplementation of low protein and/or energy diets on growth performance and blood parameters in broiler chickens under heat stress condition. Italian Journal of Animal Science, 18(1), 679-689.
Mahmood, T., Mirza, M.A., Nawaz, H. and Shahid, M., 2017. Effect of different exogenous proteases on growth performance, nutrient digestibility, and carcass response in broiler chickens fed poultry by-product meal-based diets. Livestock Science, 200, 71-75.
National Research Council, 1994. Nutrient requirements of poultry: 1994. National Academies Press.
Rada, V., Lichovníková, M., Foltyn, M. and Šafařík, I., 2016. The effect of exogenous protease in broiler diets on the apparent ileal digestibility of amino acids and on protease activity in jejunum. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(5), 1645-1652.
SAS Institute Inc. 2002. SAS/STAT User’s Guide. Version 9.1 ed. SAS Institute Inc., Cary, NC
Tang, Z., Wen, C., Li, P., Wang, T., Zhou, Y., 2014. Effect of zinc-bearing zeolite clinoptilolite on growth performance, nutrient retention, digestive enzyme activities, and intestinal function of broiler chickens. Biological trace element research. 1;158(1):51–7.
Walk, C.L., Pirgozliev, V., Juntunen, K., Paloheimo, M. and Ledoux, D.R., 2018. Evaluation of novel protease enzymes on growth performance and apparent ileal digestibility of amino acids in poultry: enzyme screening. Poultry science, 97(6), 2123-2138.
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Jabbar, A., Tahir, M., Khan, R.U. et al. Interactive effect of exogenous protease enzyme and dietary crude protein levels on growth and digestibility indices in broiler chickens during the starter phase. Trop Anim Health Prod 53, 23 (2021). https://doi.org/10.1007/s11250-020-02466-5
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DOI: https://doi.org/10.1007/s11250-020-02466-5