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The effects of molsidomine on hypoxia inducible factor alpha and Sonic hedgehog in testicular ischemia/reperfusion injury in rats

  • Urology - Original Paper
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Abstract

This study was designed to determine the effect of molsidomine (MO), a precursor of nitric oxide (NO) donor, on hypoxia inducible factor alpha (HIF-1α) and Sonic hedgehog (Shh) levels considered to be involved in the development of testes ischemia/reperfusion (I-R) injury. Torsions were created by rotating ipsilateral testes 720° in a clockwise direction for 6 h and 1-h detorsion of the testis was performed. A sham operation was performed in group 1 (control, n = 7). In group 2 (I-R/Untreated, n = 7), following 6 h of unilateral testicular torsion, 1-h detorsion of the testis was performed. No drug was given. In group 3 (I-R/MO), after performing the same surgical procedure as in group 2, a NO donor MO was given at the starting time of reperfusion. In group 4 (I-R/L-NAME), after performing the same surgical procedure as in group 2, L-NAME was given at the starting time of reperfusion. Testes malondialdehyde (MDA) levels were determined as well as examining the testes histologically. Treatment of rats with MO produced a significant reduction in the levels of MDA and histopathological score compared to testes I-R groups. The Sonic hedgehog (Shh) expression in the basement membrane of the tubuli seminiferi, and sertoli and germinal cells in testicular tissue, were greatly increased in the I-R/MO group compared to groups 1, 2 and 4. Additionally, the HIF-1α expression in the interstitial spaces in testicular tissue were greatly increased in the I-R/MO group. The results suggest that MO has a protective effect against ischemia/reperfusion injury in rat testes and may affect Shh and HIF-1α signaling pathway.

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Authors and Affiliations

  1. Department of Pediatric Surgery, Sisli Etfal Training and Research Hospital, Istanbul, Turkey

    Ali Ihsan Dokucu

  2. Department of Pediatric Surgery, Medical School, Düzce University, Duzce, Turkey

    Hulya Ozturk

  3. Department of Pediatric Surgery, Medical School, Abant Izzet Baysal University, 14280, Bolu, Turkey

    Hayrettin Ozturk

  4. Department of Anatomy, Medical School, Dicle University, Diyarbakir, Turkey

    Mehmet Cudi Tuncer

  5. Department of Pathology, Medical School, Abant Izzet Baysal University, Bolu, Turkey

    Fahri Yilmaz

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  1. Ali Ihsan Dokucu
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  2. Hulya Ozturk
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Correspondence to Hayrettin Ozturk.

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Dokucu, A.I., Ozturk, H., Ozturk, H. et al. The effects of molsidomine on hypoxia inducible factor alpha and Sonic hedgehog in testicular ischemia/reperfusion injury in rats. Int Urol Nephrol 41, 101–108 (2009). https://doi.org/10.1007/s11255-008-9460-6

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