Abstract
Glucoamylase production by a thermophilic mold Thermomucor indicae-seudaticae was optimized in solid-state fermentation (SSF) by conventional ‘one variable at a time approach’ and further statistically using response surface methodology (RSM). Glucoamylase secretion was strongly affected by three variables (moisture ratio, inoculum level and incubation time), and therefore, these three factors were further optimized using response surface methodology. The glucoamylase production in flasks containing wheat bran, under the conditions optimized by RSM, was 455 ± 23 U/g of dry moldy bran (DMB), while the predicted value by a polynomial model was 433.30 U/g DMB. The enzyme titre (455 ± 23 U/g DMB) attained in the validation experiment of this investigation is higher than those reported in the literature. When the large-scale production was attempted in enamel trays, a marginally lower enzyme titres were attained. An overall 1.8-fold increase in glucoamylase production was achieved in SSF due to statistical optimization in comparison with that of ‘one variable at a time’ approach (250 ± 13 U/g DMB). A 10-fold enhancement in glucoamylase production was recorded in SSF as compared to that in submerged fermentation.
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References
A. Archana T. Satyanarayana (1997) ArticleTitleXylanase production by thermophilic Bacillus licheniformis A99 by solid state fermentation Enzyme and Microbial Technology 21 12–17
K.R. Babu T. Satyanarayana (1995) ArticleTitleα-Amylase production by thermophilic Bacillus coagulans in solid state fermentation Process Biochemistry 30 305–309
N.O. Bartholomew S.I. Francis I.E. Lewis U.A. Chukuwudi J.C.O. Fredrick (2000) ArticleTitlePurification and some properties of a novel starch-digesting amylase from Aspergillus carbonarius Journal of the Science of Food and Agriculture 81 329–336
R.A. Battaglino M. Huegro A.M.R. Pilosof G.B. Bartholmai (1991) ArticleTitleCulture requirement for the production of protease by Aspergillus oryzae in SSF Applied Microbiology and Biotechnology 35 292–296
B. Bogar G. Szakacs A. Pandey S. Abdulhameed J.C. Linden R.P. Tengerdy (2003) ArticleTitleProduction of phytase by Mucor racemosus in solid state fermentation Biotechnology Progress 19 312–319
L.R. Castilho R.A. Medronho T.L.M. Alves (2000) ArticleTitleProduction and extraction of pectinase obtained by solid state fermentation of agro-industrial residues with Aspergillus niger Bioresource Technology 71 45–50
M. Chawla (Eds) (1996) Amylolytic enzymes of the thermophilic mould Humicola lanuginosa. M.Sc. Dissertation University of Delhi South Campus New Delhi, India.
W.G. Cochran D.W. Cox (Eds) (1968) Experimental Designs John-Wiley and Sons, Inc. New York
L.I. Dua-Chaun Y. Yi-jun P. You-Liang S. Chong-Yao (1997) ArticleTitlePurification and properties of a thermostable alpha amylase from the thermophilic fungus Thermomyces lanuginosus Weishengwu Xuebao 37 107–114
R. Emerson (1941) ArticleTitleAn experimental study of life cycle and taxonomies of Allomyces Lloydia 4 77–144
A. Gessesse G. Mamo (1999) ArticleTitleHigh level xylanase production by an alkalophilic Bacillus sp. by using solid state fermentation Enzyme and Microbiol Technology 25 68–72
N.P. Ghildyal M. Ramakrishna B.K. Lonsane N.G. Karanth (1992) ArticleTitleGaseous concentration gradient in tray type solid state fermentation-effect on yield and productivities BioProcess Engineering 8 67–72
G.V. Gonzales E.F. Torres C.N. Aguilar S.J.R. Gomez G.D. Godinez C. Augur (2002) ArticleTitleAdvantage of fungal enzyme production in solid state over liquid fermentation systems Biochemiscal Engineering Journal 3643 1–11
M.K. Gowthaman K.S.M.S. Raghava Rao N.P. Ghildyal N. Karnath (1993) ArticleTitleGas concentration and temperature gradient in a packed bed solid state fermenter Biotechnology Advances 11 611–620
M.K. Gowthaman K. Chundakkadu M. Moo-Young (2001) Fungal solid state fermentation–an overview. In Applied Mycology and Biotechnology G.G. Khachatourians D.K. Arora (Eds) Vol. 1. Agriculture and Food Production Elsevier Science The Netherlands 305–352
W. Grajek P. Gervais (1987) ArticleTitleInfluence of water activity on enzyme biosynthesis and enzyme activities produced by Trichoderma viride TS in solid state fermentation Enzyme and Microbial Technology 9 658–662
P.V. Gwande M.Y. Kamat (1999) ArticleTitleProduction of Aspergillus xylanase by lignocellulosic waste fermentation and its application Journal of Applied Microbiology 87 511–519
I. Haasum S.H. Erickson B. Jensen J. Olsen (1991) ArticleTitleGrowth and glucoamylase production by a thermophilic fungus Thermomyces lanuginosus in a synthetic medium Applied Microbiology and Biotechnology 34 656–660
S.A. Jaleel S. Srikanta N.G. Karanth (1992) ArticleTitleProduction of fungal amyloglucosidase by solid state fermentation-influence of some parameters Journal of Microbiology and Biotechnology 7 1–8
B. Jensen J. Olsen (1992) ArticleTitlePhysicochemical properties of a purified alpha amylase from the thermophilic fungus Thermomyces lanuginosus Enzyme and Microbiology Technology 14 112–116
W. Kanlaykrit K. Ishimatsu M. Nakao S. Hayashida (1987) ArticleTitleCharacteristics of raw starch-digesting glucoamylase from thermophilic Rhizomucor pusilus Journal of the Fermentation Technology 65 379–385
P. Kaur T. Satyanarayana (2001) ArticleTitlePartial purification and characterization of glucoamylase of thermophilic mold, Thermomucor indicae-seudaticae Indian Journal of Microbiology 41 195–199
S. Kumar T. Satyanarayana (2001) ArticleTitleMedium optimization for glucoamylase production by a yeast, Pichia subpelliculosa ABWF 64, in submerged cultivation World Journal of Microbiology and Biotechnology 17 83–87
S. Kumar T. Satyanarayana (2003) ArticleTitlePurification and kinetics of raw starch hydrolysing thermostable and neutral glucoamylase of thermophilic mold Thermomucor indicae-seudaticae Biotechnology Progress 19 936–944
P. Layman (1990) ArticleTitlePromising new markets emerging for commercial enzymes Chemical and Engineering News 68 17–18
B.K. Lonsane N.P. Ghildyal S. Budiatman S.V. Ramakrishna (1985) ArticleTitleEngineering aspects of solid state fermentation Enzyme and Microbial Technology 7 258–265
D.K. Maheswari H. Jahan J. Paul A. Verma (1992) ArticleTitleWheat straw, a potential substrate for cellulase production using Trichoderma reesei World Journal of Microbiology and Biotechnology 9 120–121
R. Mayer D. Montgomery (Eds) (1995) Response Surface Methodology: Process and Product Optimization using Designed Experiments. John Wiley New York
S.R. Nair S.K. Rakshit T. Panda (1995) ArticleTitleEffect of carbon sources on the synthesis of pectinase by Aspergilli BioProcess Engineering 13 37–40
A. Pandey (1990) ArticleTitleImprovement in solid-state fermentation for glucoamylase production Biological Wastes 34 11–19
A. Pandey (1995) ArticleTitleGlucoamylase: an overview Starch/Starke 47 439–445
A. Pandey L. Ashakumary P. Selvakumar K.S. Vijyalakshmi (1994) ArticleTitleInfluence of water activity on growth and activity of Aspergillus niger NCIM 1245 for enzyme production in solid state fermentation World Journal of the Microbiology and Biotechnology 10 485–486
Y.S. Park S.W. Kang J.S. Lee S.I. Hong S.W. Kim (2002) ArticleTitleXylanase production in solid state fermentation by Aspergillus niger mutant using statistical experimental design Applied Microbiology and Biotechnology 58 761–766
M.V. Ramana Murthy N.G.V. Karnath K.S.M.S. RaghavaRao (1993) ArticleTitleBiochemical engineering aspects of solid-state fermentation Advances in Applied Microbiology 38 99–147
M.V. Ramesh B.K. Lonsane (1987) ArticleTitleSolid state fermentation for production of α-amylase production by Bacillus megaterium 16M Biotechnology Letters 9 323–328
J.L.U.M. Rao T. Satyanarayana (2003) ArticleTitleStatistical optimization of a high maltose-forming, hyperthermostable and Ca2+-independent α-amylase production by an extreme thermophile Geobacillus thermoleovorans using response surface methodology Journal of Applied Microbiology 95 712–718
P.J. Reilly (1999) ArticleTitleProtein engineering of glucoamylase to improve industrial properties. A Review Starch/Starke 51 269–274
P. Selvakumar L. Ashakumari A. Helen A. Pandey (1996) ArticleTitlePurification and characterization of glucoamylase produced by Aspergilus niger in solid state fermentation Lettera in Applied Microbiology 23 403–406
C.R. Soccol I. Ilkoi B. Marin M. Raimbault (1994) ArticleTitleComparative production of α-amylase, glucoamylase and protein enrichment of raw and cooked cassava by Rhizopus strains in submerged and solid state fermentations Journal of Food Science of Technology 31 320–324
M.C.D.O. Souza I.C. Roberto A.M.F. Milagres (1999) ArticleTitleSolid state fermentation for xylanases production by Thermomascus auranticus using response surface methodology Applied Microbiology and Biotechnology 52 768–772
R. Strobel G. Sullivan (1999 ) Experimental design for improvement of fermentation. In A.L. Demain J.E. Davies (Eds) Manual of Industrial Microbiology and Biotechnology. ASM press Washington 80–93
Subrahmanyam, A., Mehrotra, B.S. & Thirumalachar 1977 Thermomucor a new genus of Mucorales. Georgia Journal of Science 35, 106.
P.M. Taylor E.J. Napier I.D. Fleming (1978) ArticleTitleSome properties of a glucoamylase produced by the thermophilic fungus Humicola lanuginosa Carbohydrate Research 61 301–308
A. Trilli I. Costnzi F. Lamanna N. DiDio (1982) ArticleTitleDevelopment of the agar disc method or the rapid screening of strain with rapid productivity Journal of Chemical Technology and Biotechnology 32 281–291
A. Vohra T. Satyanarayana (2002) ArticleTitleStatistical optimization of the medium components by response surface methodology to enhance phytase production by Pichia anomala Process Biochemistry 37 999–1004
G. Walsh (2002) Industrial enzymes G. Walsh (Eds) An introduction. In Biochemistry and Biotechnology John Wiley & Sons Ltd. New York 393–454
S. Xavier N.G. Karnath (1992) ArticleTitleA convenient method to measure water activity in solid state fermentation systems Letters in Applied Microbiology 15 53–55
F. Zadrazil H. Brunnert (1981) ArticleTitleInvestigation on physical parameters important for the SSF of straw by white rot fungi European Journal of Applied Microbiology and Biotechnology 11 183–188
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Kumar, S., Satyanarayana, T. Statistical optimization of a thermostable and neutral glucoamylase production by a thermophilic mold Thermomucor indicae-seudaticae in solid-state fermentation. World J Microbiol Biotechnol 20, 895–902 (2004). https://doi.org/10.1007/s11274-004-2891-z
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DOI: https://doi.org/10.1007/s11274-004-2891-z