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Enhanced lignin peroxidase synthesis by Phanerochaete Chrysosporium in solid state bioprocessing of a lignocellulosic substrate

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Summary

A solid state fermentation (SSF) process for the production of lignin peroxidase was optimized to enhance enzyme production by Phanerochaete chrysosporium. Optimization of the corncob SSF medium caused a significant reduction in fermentation time to give maximum lignin peroxidase yield. Supplementation of the SSF medium by low concentrations of peptone, yeast extract and Tween-80 enhanced lignin peroxidase production. Maximum yield of lignin peroxidase was 13.7 U/gds (units per gram dry substrate) noted after 5 days of SSF with 70% moisture and 20% (v/w) inoculum.

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Acknowledgments

The corresponding author was working as a Postdoctoral Fellow financed by Higher Education Commission, Islamabad, Pakistan. The facilities provided by the Department of Chemical Engineering, University of Waterloo, Ontario, Canada are highly acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, University of Agriculture, Faisalabad, Pakistan

    M. Asgher & M.J. Asad

  2. Department of Chemical Engineering, University of Waterloo, Ontario, Canada

    M. Asgher & R.L. Legge

Authors
  1. M. Asgher
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  2. M.J. Asad
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  3. R.L. Legge
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Correspondence to M. Asgher.

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Asgher, M., Asad, M. & Legge, R. Enhanced lignin peroxidase synthesis by Phanerochaete Chrysosporium in solid state bioprocessing of a lignocellulosic substrate. World J Microbiol Biotechnol 22, 449–453 (2006). https://doi.org/10.1007/s11274-005-9055-7

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  • DOI: https://doi.org/10.1007/s11274-005-9055-7

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