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Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold

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Abstract

One hundred and forty-two different actinomycete strains were isolated from rhizosphere soil of Vitis vinifera L. sampled from four Moroccan areas. To evaluate the antifungal effect of the different collected actinomycete isolates, five fungi known to be phytopathogens (Pythium ultimum, Fusarium oxyysporum f. sp. albedinis, Sclerotium rolfsii, Verticillium dahliae and Botrytis cinerea) were used. Results showed that 24 isolates had an in vitro inhibitory effect toward at least 4 of the indicator fungi, but only 9 inhibited all these phytopathogens. These nine isolates were subsequently evaluated individually using in vitro grapevine plantlets for their ability to protect against plant gray mold. We demonstrate here that pre-inoculation of plantlets with these isolates allow them to withstand Botrytis cinerea. Six of these strains were shown to belong to the genus Streptomyces and three to the genus Micromonospora. These findings indicate the potential of developing effective actinomycetes from Moroccan habitats for the biological control of Botrytis cinerea.

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Authors and Affiliations

  1. Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne, UFR Sciences—UPRES EA 2069, Université de Reims Champagne-Ardenne, 51687, Reims Cedex 2, France

    Souad Loqman, Essaid Ait Barka & Christophe Clément

  2. Faculté de Sciences Semlalia, Département de Biologie, Laboratoire de Biologie et de Biotechnologie des Microorganismes, Université Cadi Ayyad (UCAM), Avenue Prince My. Abdellah, BP 23 90, Marrakech, 40 000, Morocco

    Souad Loqman & Yedir Ouhdouch

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  1. Souad Loqman
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  2. Essaid Ait Barka
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  3. Christophe Clément
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  4. Yedir Ouhdouch
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Correspondence to Yedir Ouhdouch.

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Loqman, S., Barka, E.A., Clément, C. et al. Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold. World J Microbiol Biotechnol 25, 81–91 (2009). https://doi.org/10.1007/s11274-008-9864-6

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  • DOI: https://doi.org/10.1007/s11274-008-9864-6

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