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Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion

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Abstract

Twenty endophytic bacteria were isolated from the meristematic tissues of three varieties of strawberry cultivated in vitro, and further identified, by FAME profile, into the genera Bacillus and Sphingopyxis. The strains were also characterized according to indole acetic acid production, phosphate solubilization and potential for plant growth promotion. Results showed that 15 strains produced high levels of IAA and all 20 showed potential for solubilizing inorganic phosphate. Plant growth promotion evaluated under greenhouse conditions revealed the ability of the strains to enhance the root number, length and dry weight and also the leaf number, petiole length and dry weight of the aerial portion. Seven Bacillus spp. strains promoted root development and one strain of Sphingopyxis sp. promoted the development of plant shoots. The plant growth promotion showed to be correlated to IAA production and phosphate solubilization. The data also suggested that bacterial effects could potentially be harnessed to promote plant growth during seedling acclimatization in strawberry.

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Acknowledgements

We thank the Agroceres for supplying material and infra-structure for the laboratory of microbiology, locates at Univas (Pouso Alegre, MG, Brazil). We also thank Capes and CNPq for grants supplying for authors. In addition, we thank Francisco D. Andreote for technical supplying and critical discussions about the article.

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Authors and Affiliations

  1. Laboratory of Environmental Microbiology – Embrapa Meio Ambiente, Jaguariuna, SP, Brazil

    Armando C. F. Dias, Fernando D. Andreote & Itamar S. Melo

  2. Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, P.O. Box 83, Piracicaba, 13400-970, Brazil

    Armando C. F. Dias, Paulo T. Lacava, Laura C. Assumpção, Welington L. Araújo & João L. Azevedo

  3. Laboratory of Microbiology, University of Vale do Sapucaí –UNIVAS, Pouso Alegre, MG, Brazil

    Armando C. F. Dias, Francisco E. C. Costa & Manoel A. Teixeira

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  1. Armando C. F. Dias
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  2. Francisco E. C. Costa
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  3. Fernando D. Andreote
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  4. Paulo T. Lacava
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  5. Manoel A. Teixeira
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  6. Laura C. Assumpção
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  7. Welington L. Araújo
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  8. João L. Azevedo
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  9. Itamar S. Melo
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Correspondence to Armando C. F. Dias.

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Dias, A.C.F., Costa, F.E.C., Andreote, F.D. et al. Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion. World J Microbiol Biotechnol 25, 189–195 (2009). https://doi.org/10.1007/s11274-008-9878-0

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  • DOI: https://doi.org/10.1007/s11274-008-9878-0

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