Skip to main content

Advertisement

SpringerLink
Log in

Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

A total of 288 marine samples were collected from different locations of the Bay of Bengal starting from Pulicat lake to Kanyakumari, and 208 isolates of marine actinomycetes were isolated using starch casein agar medium. The growth pattern, mycelial coloration, production of exopolysaccharides and diffusible pigment and abundance of Streptomyces spp. were documented. Among marine actinomycetes, Streptomyces spp. were present in large proportion (88%). Among 208 marine actinomycetes, 111 isolates exhibited antimicrobial activity against human pathogens, and 151 showed antifungal activity against two plant pathogens. Among 208 isolates, 183, 157, 116, 72 and 68 isolates produced lipase, caseinase, gelatinase, cellulase and amylase, respectively. The results of diversity, antimicrobial activity and enzymes production have increased the scope of finding industrially important marine actinomycetes from the Bay of Bengal and these organisms could be vital sources for the discovery of industrially useful molecules/enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Anderson AS, Wellington EMH (2001) The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol 51:797–814

    CAS  Google Scholar 

  • Azeredo LAI, Leite SGF, Freire DMG, Benchetrit LC, Coelho RRR (2001) Proteases from actinomycetes interfere in solid media plate assays of hyaluronidase activity. J Microbiol Methods 45:207–212

    Article  Google Scholar 

  • Becker B, Lechevalier MP, Gordon RE, Lechevalier HA (1964) Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole cell hydrolysate. Appl Microbiol 12:421–424

    CAS  Google Scholar 

  • Crawford DI, Lynch JM, Whipps JM, Ousley MA (1993) Isolation and characterization of actinomycetes antagonistic to a fungal root pathogen. Appl Environ Microbiol 59:3899–3909

    CAS  Google Scholar 

  • Deshmukh MB, Sridhar KR (2002) Distribution and antimicrobial activity of actinomycetes of a fresh water coastal stream. Asian J Microbiol Biotech Environ Sci 4:335–340

    Google Scholar 

  • Duangmal K, Ward CA, Goodfellow M (2005) Selective isolation of members of the Streptomyces violaceoruber clad from soil. Microbiol Lett 245:321–327

    Article  CAS  Google Scholar 

  • Fenical W, Jensen PR (2006) Developing a new resource for drug discovery: marine actinomycete bacteria. Nat Chem Biol 2:666–673

    Article  CAS  Google Scholar 

  • Fguira L, Fotso S, Ben Ameur-Mehdi R, Mellouli L, Laatsch H (2005) Purification and structure elucidation of antifungal and antibacterial activities of newly isolated Streptomyces sp. strain US80. Res Microbiol 156:341–347

    Article  CAS  Google Scholar 

  • Fiedler HP, Bruntner C, Bull AT, Ward AC, Goodfellow M, Potterat O, Puder C, Mihm G (2005) Marine actinomycetes as a source of novel secondary metabolites. Antonie Van Leeuwenhoek 87:37–42

    Article  CAS  Google Scholar 

  • Goodfellow M, Williams ST (1983) Ecology of actinomycetes. Annu Rev Microbiol 37:189–216

    Article  CAS  Google Scholar 

  • Goshev I, Gousterova A, Vasileva-Tonkova E, Nedkov P (2005) Characterization of the enzyme complexed produced by two newly isolated thermophilic actinomycete strains during growth on collagen rich materials. Process Biochem 40:1627–1631

    Article  CAS  Google Scholar 

  • Hayakawa M, Nonomura H (1987) Humic acid vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509

    Article  CAS  Google Scholar 

  • Imada C (2004) Enzyme inhibitors and other bioactive compounds from marine actinomycetes. Antonie von Leewenhoek 87:59–63

    Article  CAS  Google Scholar 

  • Imada C, Koseki N, Kamata M, Kobayashi T, Hamada-Sato N (2007) Isolation and characterization of antibacterial substances produced by marine actinomycetes in the presence of seawater. Actinomycetologica 21:27–31

    Article  CAS  Google Scholar 

  • IMTECH (1998) Laboratory manual for identification of actinomycetes. Institute of Microbial Technology, Chandigarh, p 94

    Google Scholar 

  • Jensen P, William F (1994) Strategies for the discovery of secondary metabolites from marine bacteria. Ecological perspectives. Annu Rev Microbiol 48:559–584

    Article  CAS  Google Scholar 

  • Jensen P, Dwight R, Fenical W (1991) The distribution of actinomycetes in near-shore tropical marine sediments. Appl Environ Microbiol 57:1102–1108

    CAS  Google Scholar 

  • Jensen PR, Gontang E, Mafnas C, Mincer TJ, Fenical W (2005a) Culturable marine actinomycetes diversity from tropical Pacific ocean sediments. Environ Microbiol 7:1039–1048

    Article  Google Scholar 

  • Jensen PR, Mincer TJ, Williams PG, Fenical W (2005b) Marine actinomycete diversity and natural product discovery. Antonie Van Leeuwenhoek 87:43–48

    Article  CAS  Google Scholar 

  • Kathiresan K, Balagurunathan R, Masilamani Selvam M (2005) Fungicidal activity of marine actinomycetes against phytopathogenic fungi. Indian J Biotechnol 4:271–276

    Google Scholar 

  • Kokare CR, Mahadik KR, Kadam SS, Chopade BA (2004a) Isolation, characterization and antimicrobial activity of marine halophilic Actinopolyspora species AH1 from the west coast of India. Curr Sci 86:593–597

    Google Scholar 

  • Kokare CR, Mahadik KR, Kadam SS, Chopade BA (2004b) Isolation of bioactive marine actinomycetes from sediments isolated from Goa and Maharashtra coastlines (west coast of India). Indian J Mar Sci 33:248–256

    CAS  Google Scholar 

  • Kuster E, Williams S (1964) Selection of media for the isolation of Streptomyces. Nature 202:928–929

    Article  Google Scholar 

  • Lange L, Lopez CS (1996) Microorganisms as a source of biologically active secondary metabolites. In: Copping LG (ed) Crop protection agents from nature: natural products, analogues. The royal society of chemistry, Cambridge

    Google Scholar 

  • Lechevalier HA, Lechevalier MP (1970) A critical evaluation of the genera of aerobic actinomycetes. In: Prauser H (ed) The actinomycetes. Gustav F Ischer-Verlag, Jena, pp 393–405

    Google Scholar 

  • Lee JY, Hwang BK (2002) Diversity of antifungal actinomycetes in various vegetative soils of Korea. Can J Microbiol 48:407–417

    Article  CAS  Google Scholar 

  • Leon J, Liza L, Soto I, Cuadra D, Patino L, Zerpa R (2007) Bioactives actinomycetes of marine sediment from the central coast of Peru. Revi Peru Boil 14:259–270

    Google Scholar 

  • Lo CW, Lai NS, Cheah HY, Wong NKI, Ho CC (2002) Asian review of biodiversity and environmental conservation. http://www.arbc.com.my/pdf/art21julysep02.pdf

  • Magarvey NA, Keller JM, Bernan V, Dworkin M, Sherman DH (2004) Isolation and characterization of novel marine-derived actinomycete taxa rich in bioactive metabolites. Appl Environ Microbiol 70:7520–7529

    Article  CAS  Google Scholar 

  • Malarvizhi K (2006) Biodiversity and antagonistic potential of soil actinomycetes from south India: isolation, purification and characterization of antimicrobial metabolites produced by Streptomyces sp. MML1042. Ph.D. Thesis, University of Madras, Chennai, India

  • Maldonado LA, Fenical W, Jensen PR, Kauffman CA, Mincer TJ, Wrad AC, Bull AT, Goodfellow M (2005) Salinispora arenicola gen. nov., and Salinispora tropica nov., obligate marine actinomycetes belonging to the family Micromonosporaceae. Int J Syst Evol Microbiol 55:1759–1766

    Article  CAS  Google Scholar 

  • Maldonado LA, Fragoso-Yanez D, Pérez-García A, Rosellón-Druker J, Quintana ET (2008) Actinobacterial diversity from marine sediments collected in Mexico. Antonie van Leeuwenhoek 95:111–120. doi: 10.1007/s10482-008-9294-34

    Article  CAS  Google Scholar 

  • Mincer TJ, Jensen PR, Kauffman CA, Fenical W (2002) Wide spread and persistent populations of a major new marine actinomycete taxon in ocean sediments. Appl Environ Microbiol 68:5005–5011

    Article  CAS  Google Scholar 

  • Mohamedin AH (1999) Isolation and identification and some cultural conditions of a protease producing thermophilic Streptomyces strain grown on chicken feather as a substrate. Int J Biodeter Biodeg 43:13–21

    Article  CAS  Google Scholar 

  • Mukherjee G, Sen SK (2004) Characterization and identification of chitinase producing Streptomyces venezulae P10. Indian J Exp Biol 42:541–544

    CAS  Google Scholar 

  • Ouhdouch Y, Barakate M, Finanse C (2001) Actinomycetes of Moroccan habitats: isolation and screening for antifungal activities. Eur J Soil Biol 37:69–74

    Article  Google Scholar 

  • Pandhare J, Zog K, Deshpande VV (2002) Differential stabilities of alkaline protease inhibitors from actinomycetes: effect of various additives on thermostability. Bioresour Technol 84:165–169

    Article  CAS  Google Scholar 

  • Prabavathy VR (2005) Isolation, purification and characterization of antimicrobial metabolites produced by Streptomyces sp. and evolution against blast and sheath diseases of rice. Ph.D Thesis, University of Madras, India

  • Prabavathy VR, Mathivanan N, Murugesan K (2006) Control of blast and sheath blight diseases of rice using antifungal metabolites produced by Streptomyces sp. PM5. Biol Control 39:313–319

    Article  CAS  Google Scholar 

  • Ramesh S (2009) Marine actinomycetes diversity in Bay of Bengal, India: Isolation and characterization of bioactive compounds from Streptomyces fungicidicus MML1614. Ph. D. thesis, University of Madras, Chennai, India

  • Ramesh S, Jayaprakashvel M, Mathivanan N (2006) Microbial status in seawater and coastal sediments during pre- and post-tsunami periods in the Bay of Bengal, India. Mar Ecol 27:198–203

    Article  Google Scholar 

  • Ramesh S, Rajesh M, Mathivanan N (2009) Characterization of a thermostable alkaline protease produced by marine Streptomyces fungicidicus MML1614. Bioprocess Biosyst Eng. doi: 10.1007/s00449-009-0305-1

  • Roes LM, Meyer PR (2005) Streptomyces pharetrae sp. nov., isolated from soil from the semi-arid Karoo region. Syst Appl Microbiol 28:488–493

    Article  CAS  Google Scholar 

  • Saadoun I, Gharaibeh R (2003) The Streptomyces flora of Badia region of Jordan and its potential as a source of antibiotics active against resistant bacteria. J Arid Environ 53:365–371

    Article  Google Scholar 

  • Sharma SL, Pant A (2001) Crude oil degradation by marine actinomycetes Rhodococcus sp. Indian J Mar Sci 30:146–150

    CAS  Google Scholar 

  • Sharma AD, Kainth S, Gill PK (2005) Inulinase production using garlic (Allium sativum) powder as a potential substrate in Streptomyces sp. J Food Eng 77:1–6

    Google Scholar 

  • Stamford TLM, Stamford NP, Coelho LCBB, Araujo JM (2002) Production and characterization of a thermostable glucoamylase from Streptosporangium endophyte of maize leaves. Bioresour Technol 83:105–109

    Article  CAS  Google Scholar 

  • Sujatha P, Bapi Raju KVVSN, Ramana T (2005) Studies on a new marine Streptomycete BT-408 producing polyketide antibiotic SBR-22 effective against methicillin resistant Staphylococcus aureus. Microbiol Res 160:119–126

    Article  CAS  Google Scholar 

  • Taber WA (1960) Evidence for the existence of acid-sensitive actinomycetes in soil. Can J Microbiol 6:503

    Article  Google Scholar 

  • Takizawa M, Colwell RR, Hill RT (1993) Isolation and diversity of actinomycetes in the Chesapeake Bay. Appl Environ Microbiol 59:997–1002

    CAS  Google Scholar 

  • Tresner HD, Davies MC, Backus EJ (1961) Electron microscopy of Streptomyces spore morphology and its role in species differentiation. J Bacteriol 81:70–80

    CAS  Google Scholar 

  • Williams ST, Wilkins (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams and Wilkins, Baltimore

    Google Scholar 

  • Williams ST, Cross T (1971) Actinomycetes, methods in microbiology, vol 4. Academic Press, New York

    Google Scholar 

  • Williams ST, Goodfellow M, Alderson G (1989) Genus Streptomyces Waksman and Henrici 1943, 399AL. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 4. Williams and Wilkins, Baltimore, pp 2452–2492

    Google Scholar 

  • Yuan W, Crawford DL (1995) Characterization of Streptomyces lydicus WYEC 108 as a potential biocontrol agent against fungal root and seed rots. Appl Environ Microbiol 61:3119–3128

    CAS  Google Scholar 

  • Zaitlin B, Turkington K, Parkinson D, Clayton G (2004) Effects of tillage and inorganic fertilizers on culturable soil actinomycetes communities and inhibitors of fungi by specific actinomycetes. Appl Soil Ecol 26:53–62

    Article  Google Scholar 

  • Zhang L, An R, Wang J, Sun N, Zhang S, Hu J, Kuai J (2005) Exploring novel bioactive compounds from marine microbes. Curr Opinion Microbiol 8:276–281

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank the Director, CAS in Botany, University of Madras and the Director, National Institute of Ocean Technology, Chennai for laboratory facilities and organizing the Cruise programmes, respectively.

Author information

Authors and Affiliations

  1. Biocontrol and Microbial Metabolites Lab, Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, Tamil Nadu, 600025, India

    Subramani Ramesh & Narayanasamy Mathivanan

Authors
  1. Subramani Ramesh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Narayanasamy Mathivanan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Narayanasamy Mathivanan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ramesh, S., Mathivanan, N. Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes. World J Microbiol Biotechnol 25, 2103–2111 (2009). https://doi.org/10.1007/s11274-009-0113-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-009-0113-4

Keywords

Search

Navigation