Abstract
The aerobic nitrogen fixing xylanolytic bacterium Paenibacillus pabuli strain ATSKP produces loosely attached capsular polysaccharide KP-EPS. On 0.5% birchwood xylan 70 ± 5.02 mg of KP-EPS was produced per gram dry weight of cells by the fourth day of growth in the absence of combined nitrogen source at 30°C. It was separated and purified using centrifugation, cold acetone precipitation and dialysis and is a sulfate containing heteropolymer as revealed by FT-IR spectrometry and elemental analysis. CHN analysis revealed the presence of 37.50% carbon, 5.90% hydrogen and 8.28% nitrogen in KP-EPS. Absence of phosphorus was confirmed by 31P NMR. ICP-OES analysis showed the presence of various metals in small concentrations. Specific binding with aniline blue suggested the presence of (1,3)-β-d-glucan. Thermal gravimetric analysis and differential scanning calorimetric analysis confirmed its thermal stability as high as 200°C. The EPS was not pseudo plastic and the viscosity was less than xanthan. The intrinsic viscosity did not reduce drastically when dissolved in 0.1 M NaCl.
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Acknowledgments
We thank the technical staff of Sophisticated Analytical Instruments Facility and Department of Chemistry, IIT M. We are grateful to Dr. Susy Varughese for the viscosity measurements. Dr. Indrapal Singh Aidhen, IIT M and Dr. Roberto De Philippis, University of Florence, Italy are thanked for their valuable suggestions.
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Pooja, K.P., Chandra, T.S. Production and partial characterization of a novel capsular polysaccharide KP-EPS produced by Paenibacillus pabuli strain ATSKP. World J Microbiol Biotechnol 25, 835–841 (2009). https://doi.org/10.1007/s11274-009-9954-0
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DOI: https://doi.org/10.1007/s11274-009-9954-0