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Cladosporium sphaerospermum as a new plant growth-promoting endophyte from the roots of Glycine max (L.) Merr.

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Abstract

Endophytic fungi are plant symbionts that produce a variety of beneficial metabolites for plant growth and protection against herbivory and pathogens. Fourteen fungal samples were isolated from the roots of soybean cultivar Daemangkong and screened on waito-c rice for their plant growth-promoting capacity. Twelve of the fungal isolates promoted plant growth, while two inhibited it. The fungal isolate DK-1-1 induced maximum plant growth in both waito-c rice and soybean. The plant growth promotion capacity of DK-1-1 was higher than the wild type Gibberella fujikuroi. Gibberellin (GA) analysis of culture filtrate of DK-1-1 showed the presence of higher amounts of bioactive GA3, GA4, and GA7 (6.62, 2.1 and 1.26 ng/mL, respectively) along with physiologically inactive GA5, GA15, GA19, and GA24. Phylogenetic analysis of 18S rDNA sequence identified the fungal isolate as a new strain of Cladosporium sphaerospermum. Gibberellin production and plant growth-promoting ability of genus Cladosporium are reported for the first time in the present study.

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Acknowledgment

This study was supported by the technology development program for Agriculture and Forestry, Ministry for Agriculture, Forestry and Fisheries, Republic of Korea.

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Authors and Affiliations

  1. Division of Plant Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, Korea

    Muhammad Hamayun, Nadeem Ahmad, Dong-Sheng Tang, Sang-Mo Kang, Chae-In Na, Eun-Young Sohn, Young-Hyun Hwang, Dong-Hyun Shin & In-Jung Lee

  2. Centre of Biotechnology and Microbiology, University of Peshawar, Peshawar, Pakistan

    Sumera Afzal Khan

  3. Division of Applied Life Science, Gyeongsang National University, Jinju, Korea

    Byung-Hyun Lee

  4. Department of Life Science and Biotechnology, Kyungpook National University, Daegu, Korea

    Jong-Guk Kim

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  1. Muhammad Hamayun
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Correspondence to In-Jung Lee.

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Hamayun, M., Afzal Khan, S., Ahmad, N. et al. Cladosporium sphaerospermum as a new plant growth-promoting endophyte from the roots of Glycine max (L.) Merr.. World J Microbiol Biotechnol 25, 627–632 (2009). https://doi.org/10.1007/s11274-009-9982-9

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  • DOI: https://doi.org/10.1007/s11274-009-9982-9

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