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Effect of forced aeration on citric acid production by Aspergillus sp. mutants in SSF

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Abstract

Citric acid (CA) is one of the most important products of fermentation in the world. A great variety of agro-industrial residues can be used in solid state fermentation. Aspergillus niger parental strain (CCT 7716) and two strains obtained by mutagenesis (CCT 7717 and CCT 7718) were evaluated in Erlenmeyer flasks and glass columns using citric pulp (CP) as substrate/support, sugarcane molasses and methanol. Best results using glass columns (forced aeration) were found in the fourth day of fermentation: 278.4, 294.9 and 261.1 g CA/kg of dry CP with CCT 7716, CCT 7718 and CCT 7717, respectively. In Erlenmeyer flasks (aeration by diffusion) CA reached 410.7, 446.8 and 492.7 g CA/kg of dry CP with CCT 7716, CCT 7718 and CCT 7717, respectively. The aeration by diffusion improved CA production by the three strains. A data acquisition system specially developed for biotechnological processes analysis was used to perform the respirometric parameters measurement.

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Authors and Affiliations

  1. Bioprocess Engineering and Biotechnology Department, Federal University of Paraná (UFPR), Curitiba, PR, Brazil

    Cristine Rodrigues, Luciana P. S. Vandenberghe, Michele Rigon Spier, Júlio Cesar de Carvalho & Carlos R. Soccol

  2. Federal Institute of Parana (IFPR), Curitiba, PR, Brazil

    Wilerson Sturm

  3. Post Graduation Program in Technology, Federal Technological University of Parana (UTFPR), Curitiba, PR, Brazil

    Dario E. A. Dergint

Authors
  1. Cristine Rodrigues
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  2. Luciana P. S. Vandenberghe
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  3. Wilerson Sturm
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  4. Dario E. A. Dergint
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  5. Michele Rigon Spier
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  6. Júlio Cesar de Carvalho
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  7. Carlos R. Soccol
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Correspondence to Cristine Rodrigues.

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Rodrigues, C., Vandenberghe, L.P.S., Sturm, W. et al. Effect of forced aeration on citric acid production by Aspergillus sp. mutants in SSF. World J Microbiol Biotechnol 29, 2317–2324 (2013). https://doi.org/10.1007/s11274-013-1397-y

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  • DOI: https://doi.org/10.1007/s11274-013-1397-y

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