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Exploring extra-cellular proteins in methicillin susceptible and methicillin resistant Staphylococcus aureus by liquid chromatography–tandem mass spectrometry

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Abstract

Staphylococcus aureus (S. aureus) strains cause several diseases in humans from minor skin infections to severe lethal infections. To explore the virulence determinants of this important microorganism, two clinical isolates of methicillin susceptible S. aureus (MSSA) and methicillin resistant S. aureus (MRSA) were subjected to proteomic analysis of their extracellular products using liquid chromatography–tandem mass spectrometry. The numbers of proteins identified in MSSA and MRSA extracellular products were 168 and 261; respectively, from them 117 were shared, while 144 proteins were unique to MRSA. The shared proteins, having a higher protein score with increased number of peptide matches in MRSA over MSSA, reflect the relatively active secretory state of MRSA rather than biased analytical variances. Characteristic determinants for MRSA were identified; mostly found to play a role in the virulence. We conclude that MRSA produces distinct proteins considered as its virulence determinants and we found that the shared extracellular products are more abundant in MRSA than MSSA that supporting the high invasiveness of MRSA over MSSA in pathogenesis.

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We declare that all authors have no conflicting interests in relation to this work.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, 41522, Egypt

    Shymaa Enany

  2. Department of Structural Pathology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan

    Shymaa Enany, Yutaka Yoshida & Tadashi Yamamoto

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  1. Shymaa Enany
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  2. Yutaka Yoshida
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  3. Tadashi Yamamoto
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Correspondence to Shymaa Enany.

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Enany, S., Yoshida, Y. & Yamamoto, T. Exploring extra-cellular proteins in methicillin susceptible and methicillin resistant Staphylococcus aureus by liquid chromatography–tandem mass spectrometry. World J Microbiol Biotechnol 30, 1269–1283 (2014). https://doi.org/10.1007/s11274-013-1550-7

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  • DOI: https://doi.org/10.1007/s11274-013-1550-7

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