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Bacterioplankton community analysis in tilapia ponds by Illumina high-throughput sequencing

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Abstract

The changes of microbial community in aquaculture systems under the effects of stocking densities and seasonality were investigated in tilapia ponds. Total DNAs were extracted from the water samples, 16S rRNA gene was amplified and the bacterial community analyzed by Illumina high-throughput sequencing obtaining 3486 OTUs, from a total read of 715,842 sequences. Basing on the analysis of bacterial compositions, richness, diversity, bacterial 16S rRNA gene abundance, water sample comparisons and existence of specific bacterial taxa within three fish ponds in a 4 months period, the study conclusively observed that the dominant phylum in all water samples were similar, and they included; Proteobacteria, Cyanobacteria, Bacteroidetes, Actinobacteria, Planctomycetes and Chlorobi, distributed in different proportions in the different months and ponds. The seasonal changes had a more pronounced effect on the bacterioplankton community than the stocking densities; however some differences between the ponds were more likely caused by feed coefficient than by stocking densities. At the same time, most bacterial communities were affected by the nutrient input except phylum Cyanobacteria that was also affected by the feed control of tilapia.

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Acknowledgments

This research was jointly supported by the Special Fund of Fundamental Scientific Research Business Expense for Central Public Research Institutes (Grants. 2015JBFM12) and the China Agriculture Research System (Grants. CARS-49).

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Authors and Affiliations

  1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214182, Jiangsu Province, China

    Li Min Fan, Kamira Barry, Jia Zhang Chen & Pao Xu

  2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi, 214081, Jiangsu Province, China

    Li Min Fan, Geng Dong Hu, Shun long Meng, Chao Song, Wei Wu, Jia Zhang Chen & Pao Xu

Authors
  1. Li Min Fan
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  2. Kamira Barry
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  3. Geng Dong Hu
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  4. Shun long Meng
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  5. Chao Song
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  6. Wei Wu
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  7. Jia Zhang Chen
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  8. Pao Xu
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Correspondence to Jia Zhang Chen or Pao Xu.

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Fan, L.M., Barry, K., Hu, G.D. et al. Bacterioplankton community analysis in tilapia ponds by Illumina high-throughput sequencing. World J Microbiol Biotechnol 32, 10 (2016). https://doi.org/10.1007/s11274-015-1962-7

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  • DOI: https://doi.org/10.1007/s11274-015-1962-7

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