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Genetic mapping of Cacopsylla pyri resistance in an interspecific pear (Pyrus spp.) population

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Abstract

Cacopsylla pyri (pear psylla) is one of the most serious pests of pear (Pyrus spp.) in Europe. It can cause high yield losses, and its control has become difficult since it has developed resistance to a wide range of pesticides. Pear breeders are developing new cultivars resistant to pear psyllids, and Asian species, such as Pyrus ussuriensis and Pyrus × bretschneideri, are good sources of resistance. Antixenosis and antibiosis resistance to psylla were both identified in pear; they may differ in the biological mechanism and probably have different genetic backgrounds. We crossed interspecific P. × bretschneideri × Pyrus communis hybrid PEAR3, resistant to pear psylla, with the susceptible European pear cultivar ‘Moonglow’ to obtain an F1 population for the genetic mapping of the resistance. Quantitative trait locus (QTL) analysis was carried out for antibiosis by measuring the number of surviving nymphs and the nymphal development, using a novel phenotyping protocol and a saturated genetic map made of single-nucleotide polymorphism (SNP) and microsatellite (simple sequence repeats (SSR)) markers. A stable QTL was detected on linkage group (LG) 8 of PEAR3 (R 2 = 17.2–39.1 %). In addition, QTLs were detected on LG5 (R 2 = 10.8 %) of PEAR3 and on LG15 of ‘Moonglow’ (R 2 = 13.7 %).

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Notes

  1. The name ‘Xuehuali’ for this cultivar substitutes for the name ‘Shiyuehuali,’ which was used in Montanari et al. (2013). P. × bretschneideri ‘Xuehuali’ is also known as ‘Snowflake’ pear (Wang 2002).

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Acknowledgments

SM was funded by the Fondazione Edmund Mach (FEM) PhD School. We greatly thank Déborah Renault, Jasmine McCarthy, Ferreol Braud, Sophie Aligon, Medhi Al-Rifai, Clement Joffrion, Sylvain Hanteville, Valérie Le Mignon, Arnaud Guyader, and Christine Boursier (UMR1345 IRHS, Angers, France) for their precious help in the scoring on C. pyri eggs and nymphs. We also thank Lester Brewer at PFR for generating the PEAR3 × ‘Moonglow’ cross and the INEM team of IRHS, especially Michel Boucourt, and the INRA Experimental Unit (UE Horti, Angers, France), especially Christian Cattanéo, for taking care of the seedling population.

Data archiving statement

QTL data are reported in Table 4 and will be made publicly available through the Genome Database for Rosaceae (www.rosaceae.org).

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Authors and Affiliations

  1. Institut National de la Recherche Agronomique (INRA), UMR1345 Institut de Recherche en Horticulture et Semences-IRHS, SFR 4207 QUASAV, 42 rue Georges Morel, 49071, Beaucouzé cedex, France

    Sara Montanari, Philippe Guérif, Elisa Ravon, Caroline Denancé, Hélène Muranty, Philippe Robert, Laure Perchepied & Charles-Eric Durel

  2. Istituto Agrario San Michele all’Adige (IASMA) Research and Innovation Centre, Foundation Edmund Mach, San Michele all’Adige, Trento, Italy

    Sara Montanari & Riccardo Velasco

  3. The New Zealand Institute for Plant & Food Research Limited (Plant & Food Research), Palmerston North Research Centre, Palmerston North, New Zealand

    Sara Montanari & David Chagné

  4. Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, F-49045, Angers, France

    Philippe Guérif, Elisa Ravon, Caroline Denancé, Hélène Muranty, Philippe Robert, Laure Perchepied & Charles-Eric Durel

  5. AgroCampus-Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, 49045, Angers, France

    Philippe Guérif, Elisa Ravon, Caroline Denancé, Hélène Muranty, Philippe Robert, Laure Perchepied & Charles-Eric Durel

  6. Plant & Food Research, Hawke’s Bay Research Centre, Havelock North, New Zealand

    Vincent G. M. Bus

Authors
  1. Sara Montanari
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  2. Philippe Guérif
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  3. Elisa Ravon
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  4. Caroline Denancé
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  5. Hélène Muranty
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  6. Riccardo Velasco
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  7. David Chagné
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  8. Vincent G. M. Bus
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  9. Philippe Robert
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  10. Laure Perchepied
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  11. Charles-Eric Durel
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Corresponding author

Correspondence to Charles-Eric Durel.

Additional information

Communicated by E. Dirlewanger

Electronic supplementary material

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ESM_1

Psylla resistance phenotypic data distributions in a segregating interspecific pear population in 2013 and 2014. (PDF 189 kb)

ESM_2

Distribution of the phenotypic means of psylla resistance adjusted for environmental factors in a pear segregating population in 2013 and 2014. (PDF 321 kb)

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Montanari, S., Guérif, P., Ravon, E. et al. Genetic mapping of Cacopsylla pyri resistance in an interspecific pear (Pyrus spp.) population. Tree Genetics & Genomes 11, 74 (2015). https://doi.org/10.1007/s11295-015-0901-y

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