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Quantitative Evaluation of 2-Deoxy-2[F-18]fluoro-d-glucose-Positron Emission Tomography Imaging on the Woodchuck Model of Hepatocellular Carcinoma with Histological Correlation

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Abstract

Purpose

The Eastern woodchuck (Marmota monax) is considered as a naturally occurring animal model of hepatocellular carcinoma (HCC). The performance of 2-deoxy-2-[F-18]fluoro-d-glucose (FDG) for imaging HCC on the woodchuck using Positron emission tomography (PET) was investigated in this study.

Procedures

Dynamic FDG-PET scans were performed on five woodchucks with HCC and one healthy woodchuck before removal and processing of the liver tissues for histology. The parameters of a two-tissue compartment model with dual input were estimated using weighted least squares (WLS).

Results

Ten HCCs were confirmed histologically. Six HCCs had a tumor-to-liver standardized uptake value (SUV) ratio ≤1.15, a k 4 / k 3 ratio similar to that in hepatic tissues and were well-differentiated. Four HCCs had a tumor-to-liver SUV ratio >1.15, a lower k 4 / k 3 ratio than the hepatic tissues and were moderately differentiated.

Conclusions

Increased FDG uptake was observed in HCCs that were the least differentiated and correlated with a lower k 4 / k 3 ratio.

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Acknowledgment

We gratefully thank Joseph Molter, Gautam Desai, Jordan Dale, Anthony Megellini, and Maxwell Kotlarchyk for their kind support. We also acknowledge the laboratory animal contributions of Marmotech Inc. in the studies. This work was supported in part by the National Institute of Health (NIH CA095307).

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Authors and Affiliations

  1. Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH, 44106, USA

    Nicolas Salem, Yu Kuang, Paul W. Anderson & Zhenghong Lee

  2. Department of Pathology, University Hospitals of Cleveland, Cleveland, OH, 44106, USA

    Gregory T. MacLennan

  3. Department of Cardiothoracic Surgery, University Hospitals of Cleveland, Cleveland, OH, 44106, USA

    Steve J. Schomisch

  4. Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA

    Ilia A. Tochkov & Bud C. Tennant

  5. Department of Radiology, University Hospitals of Cleveland, 11100 Euclid Avenue, Bishop Bldg., Room S-109B, Cleveland, OH, 44106, USA

    Zhenghong Lee

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  1. Nicolas Salem
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  2. Gregory T. MacLennan
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  3. Yu Kuang
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  6. Ilia A. Tochkov
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  8. Zhenghong Lee
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Correspondence to Zhenghong Lee.

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Salem, N., MacLennan, G.T., Kuang, Y. et al. Quantitative Evaluation of 2-Deoxy-2[F-18]fluoro-d-glucose-Positron Emission Tomography Imaging on the Woodchuck Model of Hepatocellular Carcinoma with Histological Correlation. Mol Imaging Biol 9, 135–143 (2007). https://doi.org/10.1007/s11307-007-0092-5

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  • DOI: https://doi.org/10.1007/s11307-007-0092-5

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