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Antifungal efficiency of a lipopeptide biosurfactant derived from Bacillus subtilis SPB1 versus the phytopathogenic fungus, Fusarium solani

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Abstract

Bacillus subtilis SPB1 lipopeptides were evaluated as a natural antifungal agent against Fusarium solani infestation. In vitro antifungal assay showed a minimal inhibitory concentration of about 3 mg/ml with a fungicidal mode of action. In fact, treatment of F. solani by SPB1 lipopeptides generated excessive lyses of the mycelium and caused polynucleation and destruction of the related spores together with a total inhibition of spore production. Furthermore, an inhibition of germination potency accompanied with a high spore blowing was observed. Moreover, in order to be applied in agricultural field, in vivo antifungal activity was proved against the dry rot potato tubers caused by F. solani. Preventive treatment appeared as the most promising as after 20 days of fungi inoculation, rot invasion was reduced by almost 78 %, in comparison to that of non-treated one. When treating infected tomato plants, disease symptoms were reduced by almost 100 % when applying the curative method. Results of this study are very promising as it enables the use of the crude lipopeptide preparation of B. subtilis SPB1 as a potent natural fungicide that could effectively control the infection of F. solani in tomato and potato tubers at a concentration similar to the commercial fungicide hymexazol and therefore prevent the damage of olive tree.

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References

  • Al-Reza SM, Rahman A, Ahmed Y, Kang SC (2010) Inhibition of plant pathogens in vitro and in vivo with essential oil and organic extracts of Cestrum nocturnum L. Pestic Biochem Physiol 96:86–92

    Article  CAS  Google Scholar 

  • Cao Y, Xu Z, Ling N, Yuan Y, Yang X, Chen L, Shen B, Shen Q (2012) Isolation and identification of lipopeptides produced by B. subtilis SQR 9 for suppressing Fusarium wilt of cucumber. Sci Hortic 135:32–39

    Article  CAS  Google Scholar 

  • Cazorla FM, Romero D, Pérez-Garcia A, Lugtenberg BJJ, de Vicente A, Bloemberg G (2007) Isolation and characterization of antagonistic Bacillus subtilis strains from the avocado rhizoplane displaying biocontrol activity. J Appl Microbiol 103:1950–1959

    Article  CAS  Google Scholar 

  • Chitarra GS, Breeuwer P, Nout MJR, van Aelst AC, Rombouts FM, Abee T (2003) An antifungal compound produced by Bacillus subtilis YM 10–20 inhibits germination of Penicillium roqueforti conidiospores. J Appl Microbiol 94:159–166

    Article  CAS  Google Scholar 

  • Daami-Remadi M, Jabnoun-Khiareddine H, Ayed F, Hibar K, Znaidi IEA, El Mahjoub M (2006) In vitro and in vivo evaluation of individually compost fungi for potato Fusarium dry rot biocontrol. J Biol Sci 6(3):572–580

    Article  Google Scholar 

  • De Corato U, Viola E, Arcieri G, Valerio V, Cancellara FA, Zimbardi F (2014) Antifungal activity of liquid waste obtained from the detoxification of steam-exploded plant biomass against plant pathogenic fungi. Crop Prot 55:109–118

    Article  Google Scholar 

  • des Grades ZE, der Agrarwissenschaften D, Fakultät HL, Wilhelms RF (2012) Biological control of leaf pathogens of tomato plants by Bacillus subtilis (strain FZB24): antagonistic effects and induced plant resistance. Inaugural-Dissertation, Institute of Crop Science and Resource Conservation—Phytomedicine, vorgelegt am 06.06.2012

  • Ebtsam MM, Abdel-Kawi KA, Khalil MNA (2009) Efficiency of Trichodermaviride and Bacillus subtilis as biocontrol agents against Fusarium solani on tomato plants. Egypt J Phytopathol 37:47–57

    Google Scholar 

  • El-Kassas HY, Khairy HM (2009) A trial for biological control of a pathogenic fungus (Fusarium solani) by some marine microorganisms. Am Eurasian J Agric Environ Sci 5:434–440

    Article  Google Scholar 

  • Ghribi D, Abdelkefi-Mesrati L, Mnif I, Kammoun R, Ayadi I, Saadaoui I, Maktouf S, Chaabouni-Ellouze S (2012) Investigation of antimicrobial activity and statistical optimization of Bacillus subtilis SPB1 biosurfactant production in solid-state fermentation. J Biomed Biotechnol. doi:10.1155/2012/373682

    Google Scholar 

  • Hammami I, Rhouma A, Jaouadi B, Rebai A, Nesme X (2009) Optimization and biochemical characterization of a bacteriocin from a newly isolated Bacillus subtilis strain 14B for biocontrol of Agrobacterium spp. strains. Lett Appl Microbiol 48:253–260

    Article  CAS  Google Scholar 

  • Hammami I, Triki MA, Rebai A (2011) Purification and characterization of the novel bacteriocin Back IH7 with antifungal and antibacterial properties. J Plant Pathol 93:443–454

    Google Scholar 

  • Hu LB, Shi ZQ, Zhang T, Yang ZM (2007) Fengycin antibiotics isolated from B-FS01 culture inhibit the growth of Fusarium moniliforme Sheldon ATCC38932. FEMS Microbiol Lett 272:91–98

    Article  CAS  Google Scholar 

  • Jing L, Yang Q, Zhao L-H, Zhang S-M, Wang Y-X, Zhao X-Y (2009) Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29. J Zhejiang Univ Sci B 10:264–272

    Article  Google Scholar 

  • Kim PI, Bai H, Chae H, Chung S, Kim Y, Park Y, Chi Y-T (2004) Purification and characterization of a lipopeptide produced by Bacillus thuringiensis CMB26. J Appl Microbiol 97:942–949

    Article  CAS  Google Scholar 

  • Kita N, Ohya T, Uekusa H, Nomura K, Manago M, Shoda M (2005) Biological control of damping-off of tomato seedlings and cucumber Phomopsis root rot by Bacillus subtilis RB14-C. Jpn Agric Res Q 39:109–114

  • Leclère V, Béchet M, Adam A, Guez J-S, Wathelet B, Ongena M, Thonart P, Gancel F, Chollet-Imbert M, Jacques P (2005) Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism’s antagonistic and biocontrol activities. Appl Environ Microbiol 71:4577–4584

    Article  Google Scholar 

  • Leelasuphakul W, Hemmanee P, Chuenchitt S (2008) Growth inhibitory properties of Bacillus subtilis strains and their metabolites against the green mold pathogen (Penicillium digitatum Sacc.) of citrus fruit. Postharvest Biol Technol 48:113–121

    Article  CAS  Google Scholar 

  • Li L, Ma MC, Huang R, Qu Q, Li GH, Zhou JW, Zhang KQ, Lu KP, Niu XM, Luo J (2012) Induction of chlamydospore formation in Fusarium by cyclic lipopeptide antibiotics from Bacillus subtilis C2. J Chem Ecol 38:966–974

    Article  Google Scholar 

  • Lin HF, Chen TH, Liu SD (2010) Bioactivity of antifungal substance iturin A produced by Bacillus subtilis strain BS-99-H against Pestalotiopsis eugeniae, a causal pathogen of wax apple fruit rot. Plant Pathol Bull 19:225–233

    CAS  Google Scholar 

  • Matar SM, El-Kazzaz SA, Wagih EE, Al-Diwany AI, Moustafa HE, Abo-Zaid GA, Abd-Elsalam HE, Hafez EE (2009) Antagonistic and inhibitory effect of Bacillus subtilis against certain plant pathogenic fungi, I. Biotechnology 8:53–61

    Article  Google Scholar 

  • Mnif I, Sahnoun R, Ellouze-Chaabouni S, Ghribi D (2013) Evaluation of B. subtilis SPB1 biosurfactants’ potency for diesel-contaminated soil washing: optimization of oil desorption using Taguchi design. Environ Sci Pollut Res. doi:10.1007/s11356-013-1894-4

    Google Scholar 

  • Montealegre JR, Errera R, Velásquez JC, Silva P, Besoaín X, Pérez LM (2005) Biocontrol of root and crown rot in tomatoes under greenhouse conditions using Trichoderma harzianum and Paenibacillus lentimorbus. Additional effect of solarization. Electron J Biotechnol 8:250–257

    Article  Google Scholar 

  • Muyolo NG, Lipps PE, Schmitthenner AF (1993) Reactions of dry bean, lima bean, and soybean cultivars to Rhizoctonia root and hypocotyl rot and web blight. Plant Dis 77:234–238

    Article  Google Scholar 

  • Nihorimbere V, Ongena M, Cawoy H, Brostaux Y, Kakana P, Jourdan E, Thonart P (2010) Beneficial effects of Bacillus subtilis on field-grown tomato in Burundi: reduction of local Fusarium disease and growth promotion. Afr J Microbiol Res 4:1135–1142

    Google Scholar 

  • Ongena M, Jacques P (2008) Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends Microbiol 16:115–125

    Article  CAS  Google Scholar 

  • Oxenham SK, Svoboda KP, Walters DR (2005) Antifungal activity of the essential oil of basil (Ocimum basilicum). J Phytopathol 153:174–180

    Article  CAS  Google Scholar 

  • Rebib H, Hedi A, Rousset M, Boudabous A, Limam F, Sadfi-Zouaoui N (2012) Biological control of Fusarium foot rot of wheat using fengycin-producing Bacillus subtilis isolated from salty soil. Afr J Biotechnol 11:8464–8475

    CAS  Google Scholar 

  • Risoen PA, Ronning P, Hegna IK, Kolsto AB (2004) Characterization of a broad range antimicrobial substance from Bacillus cereus. J Appl Microbiol 96:648–655

    Article  CAS  Google Scholar 

  • Ruangwong OU, Chang C-I, Lamine SA, Liang W-J (2012) Identification of antifungal compound produced by Bacillus subtilisLB5 with ability to control anthracnose disease caused by Colletotrichum gloeosporioides. Afr J Microbiol Res 6:3732–3738

    CAS  Google Scholar 

  • Senthilkumar M, Swarnalakshmi K, Govindasamy V, Lee YK, Annapurna K (2009) Biocontrol potential of soybean bacterial endophytes against charcoal rot fungus, Rhizoctonia bataticola. Curr Microbiol 58:288–293

    Article  CAS  Google Scholar 

  • Soothill JS, Ward R, Girling AJ (1992) The IC50: an exactly defined measure of antibiotic sensitivity. J Antimicrob Chemother 29:137–139

    Article  CAS  Google Scholar 

  • Soylu EM, Kurt S, Soylu S (2010) In vitro and in vivo antifungal activities of the essential oils of various plants against tomato grey mould disease agent Botrytis cinerea. Int J Food Microbiol 143:183–189

    Article  CAS  Google Scholar 

  • Tawara S, Ikeda F, Maki K, Morishita Y, Otomo K, Teratani N, Goto T, Tomishima M, Ohki H, Yamada A, Kawabata K, Takasugi H, Sakane K, Tanaka H, Matsumoto F, Kuwahara S (2000) In vitro activities of a new lipopeptide antifungal agent, FK463, against a variety of clinically important fungi. Antimicrob Agents Chemother 44:57–62

    Article  CAS  Google Scholar 

  • Triki MA, Hammami I, KridHadj-Taieb S, Daami-Remadi M, Mseddi A, El Mahjoub M, Gdoura R, Khammasy N (2012) Biological control of atypical pink rot disease of potato in Tunisia. Glob Sci Books Pest Tech 6:60–64

    Google Scholar 

  • Wang J, Xia XM, Wang HY, Li PP, Wang KY (2013) Inhibitory effect of lactoferrin against gray mould on tomato plants caused by Botrytis cinerea and possible mechanisms of action. Int J Food Microbiol 161:151–157

    Article  CAS  Google Scholar 

  • Yangui T, Sayadi S, Dhouib A (2013) Sensitivity of Pectobacteriu mcarotovorum to hydroxytyrosol-rich extracts and their effect on the development of soft rot in potato tubers during storage. Crop Prot 53:52–57

    Article  CAS  Google Scholar 

  • Yun-feng Y, Qi-qin L, Gang F, Gao-qing Y, Jian-hua M, Wei L (2012) Identification of antifungal substance (Iturin A2) produced by Bacillus subtilis B47 and its effect on southern corn leaf blight. J Integ Agric 11:90–99

    Article  Google Scholar 

  • Zhang Y-L, Li S, Jiang D-H, Kong L-C, Zhang P-H, Xu J-D (2013) Antifungal activities of metabolites produced by a termite associated Streptomyces canus BYB02. J Agric Food Chem 61:1521–1524

    Article  CAS  Google Scholar 

  • Zu W, Yu H, Liang L, Fu Y, Efferth T, Liu X, Wu N (2010) Activities of ten essential oils towards Propionibacterium acnes and PC-3, A-549 and MCF-7 cancer cells. Molecules 15:3200–3210

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work has been supported by grants from the Tunisian Ministry of Higher Education, Scientific Research and Technology and the Tunisian Ministry of Agriculture.

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Authors and Affiliations

  1. Unit “Enzymes and Bioconversion”, National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia

    Ines Mnif, Semia Ellouze-Chaabouni & Dhouha Ghribi

  2. Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia

    Ines Mnif & Dhouha Ghribi

  3. Higher School of Agriculture of Kef, 7119, Kef, Tunisia

    Ines Hammami

  4. Laboratory “Amélioration et Protection des Ressources Génétiques de l’Olivier”, Sfax BP1087, Institut of Olivier, IRESA - University of Sfax, Sfax, Tunisia

    Mohamed Ali Triki & Manel Cheffi Azabou

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  1. Ines Mnif
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  2. Ines Hammami
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  4. Manel Cheffi Azabou
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  5. Semia Ellouze-Chaabouni
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Correspondence to Ines Mnif.

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Mnif, I., Hammami, I., Triki, M.A. et al. Antifungal efficiency of a lipopeptide biosurfactant derived from Bacillus subtilis SPB1 versus the phytopathogenic fungus, Fusarium solani . Environ Sci Pollut Res 22, 18137–18147 (2015). https://doi.org/10.1007/s11356-015-5005-6

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