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Bacterial biodegradation of melamine-contaminated aged soil: influence of different pre-culture media or addition of activation material

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Abstract

This study aimed to investigate the biodegrading potential of Arthrobacter sp. MCO, Arthrobacter sp. CSP, and Nocardioides sp. ATD6 in melamine-contaminated upland soil (melamine: approx. 10.5 mg/kg dry weight) after 30 days of incubation. The soil sample used in this study had undergone annual treatment of lime nitrogen, which included melamine; it was aged for more than 10 years in field. When R2A broth was used as the pre-culture medium, Arthrobacter sp. MCO could degrade 55 % of melamine after 30 days of incubation, but the other strains could hardly degrade melamine (approximately 25 %). The addition of trimethylglycine (betaine) in soil as an activation material enhanced the degradation rate of melamine by each strain; more than 50 % of melamine was degraded by all strains after 30 days of incubation. In particular, strain MCO could degrade 72 % of melamine. When the strains were pre-cultured in R2A broth containing melamine, the degradation rate of melamine in soil increased remarkably. The highest (72 %) melamine degradation rate was noted when strain MCO was used with betaine addition.

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Authors and Affiliations

  1. Organochemicals Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan

    Takashi Hatakeyama & Kazuhiro Takagi

Authors
  1. Takashi Hatakeyama
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  2. Kazuhiro Takagi
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Correspondence to Kazuhiro Takagi.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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The authors declare that they have no competing interests.

Funding

This study was funded by Foundation for Promotion of Material Science and Technology of Japan (MST Foundation).

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Responsible editor: Robert Duran

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Hatakeyama, T., Takagi, K. Bacterial biodegradation of melamine-contaminated aged soil: influence of different pre-culture media or addition of activation material. Environ Sci Pollut Res 23, 14997–15002 (2016). https://doi.org/10.1007/s11356-016-6616-2

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  • DOI: https://doi.org/10.1007/s11356-016-6616-2

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