Abstract
Pigments are present in a broad variety of terrestrial and aquatic organisms. The cannonball jellyfish (Stomolophus sp. 2) is an important fishery resource in the northwest of Mexico and is processed to be traded and consumed as seafood. During the process, water with a soluble blue pigment and other compounds are discarded to the environment. In this work, we present some properties of the blue pigment from Stomolophus sp.2 (S2bp), to decide if it could be considered as a potential value-added waste and avoid the blue proteinaceous pigment wastewater. S2bp was purified to homogeneity and had a molecular mass of 28.0 kDa; this protein exhibited a ʎmax at 650 nm, contained Zn2+ and Cu2+ metal ions, and was stable from 10 to 50 °C and in a pH range of 3.0 to 13.0 for 1 h. It had halotolerant characteristics maintaining the blue coloration in a broad range of ionic strength (0–4 M NaCl) and showed changes in ʎmax with chaotropic salts. In addition, S2bp was stable in the presence of organic acids and EDTA and in zwitterionic, anionic, and nonionic detergents at critical micellar concentration. However, oxidant reagents like NaClO and H2O2 decrease the coloration. These results show that the jellyfish pigment is a stable protein which makes it an alternative pigment for the food industry.
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Acknowledgments
We thank Luis Enrique Ordaz Cruz and Manuel Kirchmayr from CIATEJ for the support with MALDI-TOF analysis, Abigail Hernández for the jellyfish samples, Alicia Verónica Camacho Pacheco for the Stomolophus sp. 2 photographs, and Claudia Maytorena for her comments to improve the manuscript.
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Martínez-Pérez, R.B., Díaz-Tenorio, L.M., Leyva Soto, L.A. et al. Characterization of cannonball jellyfish (Stomolophus sp. 2) blue protein: a pH-stable pigment. Environ Sci Pollut Res 27, 28597–28606 (2020). https://doi.org/10.1007/s11356-020-08689-1
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DOI: https://doi.org/10.1007/s11356-020-08689-1