Abstract
Phthalates are widely used as a plasticizer in manufacturing polyethylene terephthalate (PET) bottles to improve softness, flexibility, durability, longevity, and workability. Phthalates are known in instigating profound human health hazards. In many developing countries, lack of proper disposal facilities established for empty PET bottles and the absence of legislation on reuse invariably persuade people to reuse them for storing potable water. An experiment was conducted with two commercial brands of PET bottles to explore the potential of phthalate migration when domestically refilled and reused in multiple times at two temperature conditions. Temperatures of ambient (27 ± 2 °C) and warm (60 ± 2 °C) were selected as the refilling temperatures because of the common practice by people. For both brands, only bis(2-ethylhexyl) phthalate (DEHP) levels were detected in refilled water in every event of reuse. For both brands, mean DEHP levels migrated to water at 60 ± 2 °C were significantly higher (p < 0.05) compared to those at 27 ± 2 °C. Risk analyses carried out on human health suggested that there exist no definite acute or chronic health risks when the refilled water is consumed continuously for 30 years for both temperatures. Still, such risks were higher for the consumption of refilled water of warm temperatures than those of ambient temperature. However, this study elucidates that DEHP migration would be at an alarming rate when the events of reuse of a single bottle increase so that regulations banning the reuse of empty PET bottles are paramount, especially for developing countries.
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References
Absalan F, Saremy S, Mansori E, Taheri Moghadam M, Eftekhari Moghadam AR, Ghanavati R (2017) Effects of mono-(2-ethylhexyl) phthalate and Di-(2-ethylhexyl) phthalate administrations on oocyte meiotic maturation, apoptosis and gene quantification in mouse model. Cell J 18:503–513. https://doi.org/10.22074/cellj.2016.4717
Ajji A, Cole KC, Dumoulin MM, Brisson J (1995) Amorphous orientation of poly (ethylene terephthalate) by X-ray diffraction in combination with Fourier transform infra-red spectroscopy. Polymer 36:4023–4030. https://doi.org/10.1016/0032-3861(95)90981-7
Al-Saleh I, Shinwari N, Alsabbaheen A (2011) Phthalates residues in plastic bottled waters. J Toxicol Sci 36:469–478. https://doi.org/10.2131/jts.36.469
Alzuhairi MAH, Al-Ghaban AM, Almutalabi SN (2016) Chemical recycling of polyethylene terephthalate (PET) as additive for asphalt. Zanco J Pure Appl Sci 28. https://doi.org/10.1051/matecconf/201816201042
Amiridou D, Voutsa D (2011) Alkylphenols and phthalates in bottled waters. J Hazard Mater 185:281–286. https://doi.org/10.1016/j.jhazmat.2010.09.031
ATSDR (2011) Toxicologicalprofile for Di (2-ethylhexyl) phthalate (DEHP). Agency for Toxic Substances and Disease Registry. https://www.atsdr.cdc.gov/substances/toxsubstance.asap?=65#12, Accessed 2 June 2017
Aydemir D, Karabulut G, Şimşek G, Gok M, Barlas N, Ulusu NN (2018) Impact of the di (2-Ethylhexyl) phthalate administration on trace element and mineral levels in relation of kidney and liver damage in rats. Biol Trace Elem Res 186:474–488. https://doi.org/10.1007/s12011-018-1331-0
Bach C, Dauchy X, Chagnon MC, Etienne S (2012) Chemical compounds and toxicological assessments of drinking water stored in polyethylene terephthalate (PET) bottles: a source of controversy reviewed. Water Res 46:571–583. https://doi.org/10.1016/j.watres.2011.11.062
Baldenebro-Lopez FJ, Castorena-Gonzalez J, Velazquez-Dimas J, Ledezma-Sillas J, Gómez-Esparza C, Martinez-Sanchez R, Herrera-Ramirez J (2014) Influence of continuous plastic fibers reinforcement arrangement in concrete strengthened. IOSR J Eng 4:15–23. https://doi.org/10.9790/3021-04411523
Baram GI, Azarova IN, Gorshkov AG, Vereshchagin AL, Lang B, Kiryukhina ED (2000) Determination of bis (2-ethylhexyl) phthalate in water by high-performance liquid chromatography with direct on-column preconcentration. J Anal Chem 55:750–754. https://doi.org/10.1007/BF02757910
Begley TH, Biles JE, Cunningham C, Piringer O (2004) Migration of a UV stabilizer from polyethylene terephthalate (PET) into food simulants. Food Addit Contam 21:1007–1014. https://doi.org/10.1080/02652030400010447
Biscardi D, Monarca S, De Fusco R, Senatore F, Poli P, Buschini A, Zani C (2003) Evaluation of the migration of mutagens/carcinogens from PET bottles intomineral water by Tradescantia/micronuclei test, Comet assay on leukocytes and GC/MS. Sci Total Environ 302:101–108. https://doi.org/10.1016/S0048-9697(02)00349-2
Boas M, Frederiksen H, Feldt-Rasmussen U, Skakkebæk NE, Hegedüs L, Hilsted L, Juul A, Main KM (2010) Childhood exposure to phthalates: associations with thyroid function, insulin-like growth factor I, and growth. Environ Health Perspect 118:1458–1464. https://doi.org/10.1289/ehp.0901331
Bornehag CG, Nanberg E (2010) Phthalate exposure and asthma in children. Int J Androl 33:333–345. https://doi.org/10.1111/j.1365-2605.2009.01023.x
Bošnir J, Puntarić D, Galić A, Škes I, Dijanić T, Klarić M, Grgić M, Čurković M, Šmit Z (2007) Migration of phthalates from plastic containers into soft drinks and mineral water. Food Technol Biotechnol 45:91–95 https://hrcak.srce.hr/30442
Cao XL (2010) Phthalate esters in foods: sources, occurrence, and analytical methods. Compr Rev Food Sci Food Saf 9:21–43. https://doi.org/10.1111/j.1541-4337.2009.00093.x
Casajuana N, Lacorte S (2003) Presence and release of phthalic esters and otherendocrine disrupting compounds in drinking water. Chromatographia 57:649–655. https://doi.org/10.1007/BF02491744
Chen JA, Liu H, Qiu Z, Shu W (2008) Analysis of di-n-butyl phthalate and other organic pollutants in Chongqing women undergoing parturition. Environ Pollut 156:849–853. https://doi.org/10.1016/j.envpol.2008.05.019
Coates J (2006) Interpretation of infrared spectra, a practical approach. Encyclopedia of Analytical Chemistry: Applications, Theory and Instrumentation https://doi.org/10.1002/9780470027318.a5606
Demirel B, Yaraş A, Elçiçek H (2011) Crystallization behavior of PET materials. BAUN Fen Bil Enst Dergisi 13:26–35. http://fbed.balikesir.edu.tr/index.php/dergi/article/view/33
Diduch M, Polkowska Z, Namiesnik J (2013) Factors affecting the quality of bottled water. J Expo Sci Environ Epidemiol 23:111–119. https://doi.org/10.1038/jes.2012.101
EFSA (2013) Opinion of the scientific panel on food additives, flavorings, processing aids and materials in contact with food (AFC) related to bis(2-ethylhexyl) phthalate (DEHP) for use in food contact materials. http://www.efsa.europa.eu/de/efsajournal/pub/243.htm2013. Aaccessed 15 April 2018 https://doi.org/10.2903/j.efsa.2005.243
Erkekoglu P, Zeybek ND, Giray BK, Rachidi W, Kızılgün M, Hininger-Favier I, Favier A, Asan E, Hincal F (2014) The effects of di (2-ethylhexyl) phthalate on rat liver in relation to selenium status. Int J Clin Exp Pathol 95:64–77. https://doi.org/10.1111/iep.12059
Farhoodi M, Emam-Djomeh Z, Ehsani MR, Oromiehie A (2008) Effect ofenvironmental conditions on the migration of di (2-ethylhexyl) phthalate from PETbottles into yogurt drinks: influence of time, temperature, and food simulant. Arab J Sci Eng 33:279–287
Gao Y, An T, Ji Y, Li G, Zhao C (2015) Eco-toxicity and human estrogenic exposure risks from OH-initiated photochemical transformation of four phthalates in water: a computational study. Environ Pollut 206:510–517. https://doi.org/10.1016/j.envpol.2015.08.006
González GP, Hernando PF, Alegria JD (2006) A morphological study of molecularly imprinted polymers using the scanning electron microscope. Anal Chim Acta 557:179–183. https://doi.org/10.1016/j.aca.2005.10.034
Hadjizadeh A, Ajji A, Bureau MN (2011) Nano/micro electro-spun polyethylene terephthalate fibrous mat preparation and characterization. J Mech Behav Biomed Mater 4:340–351. https://doi.org/10.1016/j.jmbbm.2010.10.014
Hauser R, Calafat AM (2005) Phthalates and human health. Occup Environ Med 62:806–818. https://doi.org/10.1136/oem.2004.017590
Hauser R, Meeker JD, Singh NP, Silva MJ, Ryan L, Duty S, Calafat AM (2007) DNA damage in human sperm is related to urinary levels of phthalate monoester and oxidative metabolites. Hum Reprod 22:688–695. https://doi.org/10.1093/humrep/del428
Heudorf U, Mersch-Sundermann V, Angerer J (2007) Phthalates: toxicology and exposure. Int J Hyg Environ Health 210:623–634. https://doi.org/10.1016/j.ijheh.2007.07.011
Higgins F (2013) Rapid and reliable phthalate screening in plastics by portable FTIR spectroscopy. Agilent Technologies (Application note 5991.3649 EN). https://www.agilent.com/cs/library/applications/5991-3649EN.pdf. Accessed 27 June 2017
Hirayama K, Tanaka H, Kawana K, Nakazawa H (2001) Analysis of plasticizers in cap-sealing resins for bottled foods. Food Addit Contam 18:357–362. https://doi.org/10.1080/02652030119099
Kamrin MA (2009) Phthalate risks, phthalate regulation, and public health: a review. J Toxicol Environ Health B Crit Rev 12:157–174. https://doi.org/10.1080/10937400902729226
Keresztes S, Tatár E, Czégény Z, Záray G, Mihucz VG (2013) Study on theleaching of phthalates from polyethylene terephthalate bottles into mineral water. Sci Total Eviron 458-460:451–458. https://doi.org/10.1016/j.scitotenv.2013.04.056
Latini G (2005) Monitoring phthalate exposure in humans. Clin Chim Acta 361:20–29. https://doi.org/10.1016/j.cccn.2005.05.003
Lee BM, Koo HJ (2007) Hershberger assay for antiandrogenic effects of phthalates. J Toxicol Environ Health A 70:1365–1370. https://doi.org/10.1080/15287390701432285
Leivadara SV, Nikolaou AD, Lekkas TD (2008) Determination of organic compounds in bottled waters. Food Chem 108:277–286. https://doi.org/10.1016/j.foodchem.2007.10.031
Liu HC, Den W, Chan SF, Kin KT (2008) Analysis of trace contamination of phthalate esters in ultrapure water using a modified solid-phase extraction procedure and automated thermal desorption–gas chromatography/mass spectrometry. J Chromatogr A 1188:286–294. https://doi.org/10.1016/j.chroma.2008.02.078
Liu X, Shi J, Bo T, Li H, Crittenden JC (2015) Occurrence and risk assessment of selected phthalates in drinking water from waterworks in China. Environ Sci Pollut Res 22:10690–10698. https://doi.org/10.1007/s11356-015-4253-9
Mallick B (2015) X-ray diffraction analysis of polymeric solid using Bragg-Brentano geometry. Int J Mater Chem Phys 1:265–270
Mariana M, Feiteiro J, Verde I, Cairrao E (2016) The effects of phthalates in the cardiovascular and reproductive systems: a review. Environ Int 94:758–776. https://doi.org/10.1016/j.envint.2016.07.004
Meeker JD, Calafat AM, Hauser R (2007) Di(2-ethylhexyl) phthalate metabolites may alter thyroid hormone levels in men. Environ Health Perspect 115:1029–1034. https://doi.org/10.1289/ehp.9852
Meeker JD, Sathyanarayana S, Swan SH (2009) Phthalates and other additives in plastics: human exposure and associated health outcomes. Philos Trans R Soc B 364:2097–2113. https://doi.org/10.1098/rstb.2008.0268
Montuori P, Jover E, Morgantini M, Bayona JM, Triassi M (2008) Assessing human exposure to phthalic acid and phthalate esters from mineral waterstored in polyethylene terephthalate and glass bottles. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25:511–518. https://doi.org/10.1080/02652030701551800
Nunes RAX, Costa VC, Calado VMDA, Branco JRT (2009) Wear, friction, and microhardness of a thermal sprayed PET: poly (ethylene terephthalate) coating. Mater Res 12:121–125. https://doi.org/10.1590/S1516-14392009000200002
Penalver A, Pocurull E, Borrull F, Marce RM (2000) Determination of phthalate esters in water samples by solid-phase microextraction and gas chromatography with mass spectrometric detection. J Chromatogr A 872:191–201. https://doi.org/10.1016/S0021-9673(99)01284-4
Pinto B, Reali D (2009) Screening of estrogen-like activity of mineral water stored in PET bottles. Int J Hyg Environ Health 212:228–232. https://doi.org/10.1016/j.ijheh.2008.06.004
Plotan M, Frizzell C, Robinson V, Elliott CT, Connolly L (2013) Endocrine disruptor activity in bottled mineral and flavoured water. Food Chem 136:1590–1596. https://doi.org/10.1016/j.foodchem.2012.01.115
Pocar P, Fiandanese N, Berrini A, Secchi C, Borromeo V (2017) Maternal exposure to di (2-ethylhexyl) phthalate (DEHP) promotes the transgenerational inheritance of adult-onset reproductive dysfunctions through the female germline in mice. Toxicol Appl Pharmacol 322:113–121. https://doi.org/10.1016/j.taap.2017.03.008
Saylor A, Prokopy LS, Amberg S (2011) What’s wrong with the tap? Examining perceptions of tap water and bottled water at Purdue University. J Environ Manag 48:588–601. https://doi.org/10.1007/s00267-011-9692-6
Schmid P, Kohler M, Meierhofer R, Luzi S, Wegelin M (2008) Does the reuse of PET bottles during solar water disinfection pose a health risk due to the migration of plasticisers and other chemicals into the water? Water Res 42:5054–5060. https://doi.org/10.1016/j.watres.2008.09.025
Serôdio P, Nogueira JMF (2006) Considerations on ultra-trace analysis of phthalates in drinking water. Water Res 40:2572–2582. https://doi.org/10.1016/j.watres.2006.05.002
Singh D, Malik HK, Gupta CK, Singh V (2017) X-ray diffraction studies for identification of polyethylene terephthalate fibres. Indian J Sci Technol 10:17. https://doi.org/10.17485/ijst/2017/v10i17/110232
Starkweather Jr HW, Zoller P, Jones GA, Vega AJ (1982) The heat of fusion of polytetrafluoroethylene. J Polym Sci Pol Phys 20:751–761. https://doi.org/10.1002/pol.1982.180200416
Toft G, Jönsson BA, Lindh CH, Jensen TK, Hjollund NH, Vested A, Bonde JP (2012) Association between pregnancy loss and urinary phthalate levels around the time of conception. Environ Health Perspect 120:458–463. https://doi.org/10.1289/ehp.1103552
Turnbull L, Liggat JJ, MacDonald WA (2013) Thermal degradation chemistry of poly (ethylene naphthalate)-a study by thermal volatilisation analysis. Polym Degrad Stab 98:2244–2258. https://doi.org/10.1016/j.polymdegradstab.2013.08.018
USEPA (1987) Integrated Risk Information System https://cfpub.epa.gov/ncea/iris2/chemicalLanding.cfm?substance_nmbr=14. Accessed 20 April 2020
USEPA (1989) Risk assessment guidance for superfund: volume I-human health evaluation manual (part A). Office of Emergency and Remedial Response, Washington, DC https://wwwepagov/sites/production/files/2015-09/documents/rags_apdf Aaccessed 13 May 2018
USEPA (2012) Phthalates Action Plan1 http://www.epa.gov/oppt/existingchemicals/pubs/actionplans/phthalates_actionplan_revised_2012-03-14.pdf.
Wang W, Wu QY, Wang C, He T, Hu HY (2015) Health risk assessment of phthalate esters (PAEs) in drinking water sources of China. Environ Sci Pollut Res 22:3620–3630. https://doi.org/10.1007/s11356-014-3615-z
Wu B, Zhang Y, Zhang X, Cheng S (2010) Health risk from exposure of organic pollutants through drinking water consumption in Nanjing, China. Bull Environ Contam Toxicol 84:46–50. https://doi.org/10.1007/s00128-009-9900-8
Wu B, Zhang Y, Zhang XX, Cheng SP (2011) Health risk assessment of polycyclic aromatic hydrocarbons in the source water and drinking water of China: quantitative analysis based on published monitoring data. Sci Total Environ 410:112–118. https://doi.org/10.1016/j.scitotenv.2011.09.046
Wu MT, Wu CF, Wu JR, Chen BH, Chen EK, Chao MC, Liu CK, Ho CK (2012) The public health threat of phthalate-tainted foodstuffs in Taiwan: the policies the government implemented and the lessons we learned. Environ Int 44:75–79. https://doi.org/10.1016/j.envint.2012.01.014
Wypych G (2004) Plasticizers use and selection for specific polymers. In: Handbook of plasticizers. Toronto, Canada, pp 273–360
Acknowledgments
The authors wish to acknowledge all personnel who helped the laboratory analyses carried out at the University of Moratuwa.
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This work was supported by the University Research Grant of University of Sri Jayewardenepura, Sri Lanka (Grant No: ASP/01/RE/SCI/2017/12), and the National Research Council (NRC), Sri Lanka (Grant Nos. 15-056 and 12-086).
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Jayaweera, M., Perera, H., Bandara, N. et al. Migration of phthalates from PET water bottle in events of repeated uses and associated risk assessment. Environ Sci Pollut Res 27, 39149–39163 (2020). https://doi.org/10.1007/s11356-020-09925-4
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DOI: https://doi.org/10.1007/s11356-020-09925-4