Abstract
The chemical composition of the essential oils obtained from petals, leaves and stems of the flowering stage of plants of Artemisia annua cultivar Jwarharti was analyzed by gas chromatography mass spectrometry (GC-MS); 86, 77, and 63 compounds were identified in the leaf, petal, and stem oils, respectively. Large compositional differences were detected between the three oils. The petal and leaf oils were rich in monoterpenes. The stem oil was rich in sesquiterpenes. Camphor, present in the leaf oil, was absent from petal oil.
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Bailey NJC, Wang Y, Sampson J, Davis W, Whitecombe I, Hylands PJ, Croft SL, Holmes E (2004) Prediction of anti-plasmodial activity of Artemisia annua extracts: application of 1H NMR spectroscopy and chemometrics. J Pharm Biomed Anal 35:117–126
Mueller MS, Runyambo N, Wagner I, Borrman S, Dietz K, Heide L (2004) Randomized controlled trial of a traditional preparation of Artemisia annua L. (annual wormwood) in the treatment of malaria. Trans R Soc Trop Med Hyg 98:318–321
Klayman DL (1985) Qinghaosu (artemisinin): an antimalarial drug from China. Science 228:1049–1055
Enserink M (2005) Source of new hope against malaria is in short supply. Science 307:33
White NJ (1999) Antimalarial drug resistance and combination chemotherapy. Philos Trans R Soc Lond Ser B 354:739–749
Olliaro PL, Taylor WRJ (2003) Antimalarial compounds: from bench to bedside. J Exp Biol 206:3753–3759
Kumar S, Srivastava S (2005) Establishment of artemisinin combination therapy as first line treatment for combating malaria: Artemisia annua cultivation in India needed for providing sustainable supply chain of artemisinin. Curr Sci 89:1097–1102
Kumar S (2005) Prospects for sustainable agricultural production of the antimalarial molecule artemisinin in India. Natl Acad Sci Lett 28:325–338
Abdin MZ, Israr M, Rehman RV, Jain SK (2003) Artemisinin, a novel antimalarial drug: biochemical and molecular approaches for enhanced production. Planta Med 69:289–293
Bertea CM, Freije JR, Van der Woude, Verstappen FWA, Perk L, Marquez V, De Kraker JW, Posthumus MA, Jansen BJM, de Groot A, Franssen MCR, Bouwmeester HJ (2004) Identification of intermediates and enzymes involved in the early steps of artemisinin biosynthesis in Artemisia annua. Planta Med 71:40–47
Picaud S, Olofsson L, Bordelius M, Bordelius P (2005) Expression, purification, and characterization of recombinant amorpha-4,11-diene synthase from Artemisia annua. Arch Biochem Biophys 436:215–226
Tang HQ, Hu J, Yang L, Tan RX (2000) Terpenoids and flavonoids from Artemisia species. Planta Med 66:391–393
Holm Y, Laosko I, Hiltunen R, Galambosi B (1997) Variation in the essential oil composition of essential oil of Artemisia annua L. of different origin cultivated in Finland. Flav Frag J 12:241–246
Adams RP (1969) Identification of essential oil components by ion trap mass spectrometry. Academic, New York
Jennings W, Shibamoto T (1980) Qualitative analysis of flavor and fragrance volatiles by glass capillary gas chromatography. Academic, New York
Davies NW (1990) Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicone and carbowax 20M phases. J Chromatogr 503:1–24
Ramaswamy SK, Brisesce P, Gargiullo RJ, van Geldern T (1988) Sesquiterpene hydrocarbons from mass confusion to orderly line-up. In: Lawrence BM, Mookherjee BD, Willis BS (eds) Flavours and fragrances: a world perspective. Elsevier, Amsterdam pp 951–976
Weyerstahl P, Marschall H, Schroder M, Wahlburg HC, Kaul VK (1997) The sesquiterpene fraction of the essential oil of Artemisia laciniata Willd. Flav Frag J 12:315–325
Libbey LM, Sturtz G (1989) Unusual essential oil grown in Oregon. II. Artemisia annua L. J Essent Oil Res 1:201–202
Charles DJ, Cebert E, Simon JE (1991) Characterization of the essential oil of Artemisia annua L. J Essent Oil Res 3:33–39
Chalchat J-C, Garry RP, Michet M, Gornnovic M, Stosic D (1991) A contribution to chemotaxonomy of Artemisia annua L. Asteraceae. Acta Pharm Jugoslav 41:233–236
Woerdenbag HJ, Bos R, Salomons MC, Hendriks H, Pras N, Malingre TM (1993) Volatile constituents of Artemisia annua L. (Asteraceac). Flav Frag J 8:131–137
Woerdenbag HJ, Pras N, Chan NG, Bang BT, Bos R, van Uden W, Y PV, Boi NV, Batterman S, Lugt CB (1994) Artemisinin, related sesquiterpenes, and essential oil in Artemisia annua during a vegetation period in Vietnam. Planta Med 60:272–275
Chalchat J-C, Garry RP, Lamy J (1994) Influence of harvest time on yield and composition of Artemisia annua oil produced in France. J Essent Oil Res 6:261–268
Ahmad A, Misra LN (1994) Terpenoids from Artemisia annua and constituents of its essential oil. Phytochemistry 37:183–186
Ram M, Gupta MM, Naqvi AA, Kumar S (1997) Effect of planting time on the yield of essential oil and artemisinin in Artemisia annua under subtropical conditions. J Essent Oil Res 9:193–197
Holm Y, Laakso I, Hiltunen R, Galambosi B (1997) Variation in the essential oil composition of Artemisia annua L. of different origin cultivated in Finland. Flav Frag J 12:241–246
Jain N, Srivastava SK, Aggarwal KK, Kumar S, Syamasunder KV (2002) Essential oil composition of Artemisia annua L. Asha from the plains of northern India. J Essent Oil Res 14:305–307
Tripathi AK, Prajapati V, Aggarwal KK, Khanuja SPS, Kumar S (2000) Repellency and toxicity of oil from Artemisia annua to certain stored-product beetles. J Econ Entomol 93:43–47
Juteau F, Masotti V, Bessiere JM, Dharbomez M, Viano J (2002) Antibacterial and antioxidant activities of Artemisia annua essential oil. Fitotrepia 73:532–535
Cha J-D, Jeong M-R, Jeong S-I, Moon S-E, Kim J-Y, Kil B-S, Song Y-H (2005) Chemical composition and antimicrobial activity of the essential oils of Artemisia scoparia and A. capillaris. Planta Med 71:186–190
Acknowledgments
Grateful thanks are due to the directors of the Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, for extending their analytical facilities, and of the National Center for Plant Genome Research for other facilities.
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Goel, D., Singh, V., Ali, M. et al. Essential oils of petal, leaf and stem of the antimalarial plant Artemisia annua . J Nat Med 61, 187–191 (2007). https://doi.org/10.1007/s11418-006-0112-9
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DOI: https://doi.org/10.1007/s11418-006-0112-9