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Antileishmanial amides and lignans from Piper cubeba and Piper retrofractum

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Abstract

The n-hexane, ethyl acetate, methanol, and acetone extracts of Piper cubeba Linn. and P. retrofractum Vahl. (Piperaceae) were evaluated in vitro against promastigotes of Leishmania donovani, and all exhibited significant in vitro activity at 100 μg/ml. Two lignans, cubebin and hinokinin, were isolated from the hexane extract of P. cubeba; and one bis-epoxy lignan, (−)-sesamin, and two amides, pellitorine and piplartine, were isolated from the hexane and methanol extracts of P. retrofractum. Cubebin and piplartine showed significant antileishmanial activity in vitro at 100 μM and were further tested in vivo in a hamster model of visceral leishmaniasis. Piplartine showed activity at 30 mg/kg dose. This is the first report of antileishmanial activity of these two plants and their isolated constituents.

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Acknowledgments

We express our sincere thanks to Prof. P. Ramarao, director, NIPER, for continuous support for this work. HSB is thankful to NIPER for fellowship. Technical assistance from Mr. Rakesh Kumar and Mr. K.S. Singh is gratefully acknowledged. GS is thankful to CSIR, New Delhi for fellowship.

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Authors and Affiliations

  1. Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Punjab, 160062, India

    Hardik S. Bodiwala, Kamlesh Kumar Bhutani & Inder Pal Singh

  2. Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Punjab, 160062, India

    Gaganmeet Singh, Ranvir Singh & Chinmoy Sankar Dey

  3. Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Punjab, 160062, India

    Shyam Sundar Sharma

Authors
  1. Hardik S. Bodiwala
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  2. Gaganmeet Singh
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  3. Ranvir Singh
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  4. Chinmoy Sankar Dey
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  5. Shyam Sundar Sharma
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  6. Kamlesh Kumar Bhutani
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  7. Inder Pal Singh
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Correspondence to Inder Pal Singh.

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NIPER Communication No. 395.

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Bodiwala, H.S., Singh, G., Singh, R. et al. Antileishmanial amides and lignans from Piper cubeba and Piper retrofractum . J Nat Med 61, 418–421 (2007). https://doi.org/10.1007/s11418-007-0159-2

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  • DOI: https://doi.org/10.1007/s11418-007-0159-2

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