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Isolation, structural elucidation and in vitro hepatoprotective activity of flavonoids from Glycyrrhiza uralensis

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Abstract

Two new flavonoid glycosides, 2′,4′-dihydroxydihydrochalcone-4-O-β-d-glucopyranoside (1) and medicarpin-3-O-β-d-apiofuranosyl (1 → 2)-β-d-glucopyranoside (2), together with 34 known flavonoids were isolated from the 75% EtOH extract of the dried roots of Glycyrrhiza uralensis Fisch. Their structures were elucidated on the basis of spectroscopic analyses. The flavonoids were classified into ten sub-types, namely, dihydrochalcone (1), pterocarpans (2–4), flavones (5–6), flavanones (7–11), chalcones (12–15), retro-chalcones (16–18), isoflavans (19–21), isoflavones (22–28), 3-arylcoumarins (29–30), and coumestans (31–36). The isolated flavonoids were evaluated for in vitro hepatoprotective activity against d-galactosamine-induced toxicity in human hepatoma HepG2 cells.

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Acknowledgements

This work was supported by State Key Program of National Natural Science Foundation of China (Grant number: 81430095).

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  1. Ming Cheng and Liqin Ding contributed equally to this work.

Authors and Affiliations

  1. School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, No. 312 Anshanxi Road, Nankai District, Tianjin, 300193, China

    Ming Cheng, Hongmin Zhang, Yingjie Sun, Wei Li & Feng Qiu

  2. Tianjin Key Laboratory of TCM Chemistry and Analysis, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China

    Ming Cheng, Liqin Ding, Hongmin Zhang, Benke Jiang, Yingjie Sun, Shijie Cao & Feng Qiu

  3. Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba, 274-8510, Japan

    Wei Li & Kazuo Koike

  4. Shenyang Pharmaceutical University, Shenyang, China

    Hongfeng Kan & Benke Jiang

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  1. Ming Cheng
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Cheng, M., Ding, L., Kan, H. et al. Isolation, structural elucidation and in vitro hepatoprotective activity of flavonoids from Glycyrrhiza uralensis. J Nat Med 73, 847–854 (2019). https://doi.org/10.1007/s11418-019-01329-0

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