Abstract
The detection of illicit drugs is important both in the management of substance misuse and in the postmortem identification of drug abuse. Urinary screening assays have been available for many years, and have been successfully utilized for detecting misuse. Urine remains the biological tool of choice to verify drug-free behavior because of noninvasive sampling and high concentrations of drugs and metabolites. Herein we describe the cross-reactivities of 39 new amphetamine designer drugs on three different on-site abuse drugs urinary screening tests (Screen®7, SureStep™, InstAlert™), which are commonly available in forensic laboratories and in the clinical environment. They are chromatographic immunoassay tests for the qualitative detection of amphetamine, methamphetamine, and methylenedioxymethamphetamine at the recommended cutoff according to Italian legislation (500 ng/ml). The speed and sensitivity of these tests have made them the most widely accepted method to screen urine for drugs of abuse.
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References
Henderson GL (1998) Designer drugs: past history and future prospects. J Forensic Sci 33:569–575
Carroll FI, Lewin AH, Mascarella SW, Seltzman HH, Reddy PA (2012) Designer drugs: a medicinal chemistry perspective. Ann NY Acad Sci 1248:18–38
United Nation Office on Drug and Crime (2011) Amphetamine and ecstasy, Global ATS Assessment
Wada M, Sugimoto Y, Crabtree BL, Evans C, Montgomery JH, Ikeda R, Kuroda N, Nakashima K (2013) Simultaneous determination of amphetamine-type stimulants in abusers’ hair: clinical usefulness of hair analysis in prehospitalization for abusers. Forensic Toxicol 31:2–8
Pirisi MA, Nieddu M, Burrai L, Carta A, Briguglio I, Baralla E, Demontis MP, Varoni MV, Boatto G (2013) An LC–MS–MS method for quantitative analysis of six trimethoxyamphetamine designer drugs in rat plasma, and its application to a pharmacokinetic study. Forensic Toxicol 31:197–203
Nieddu M, Burrai L, Pirisi MA, Carta A, Briguglio I, Dessì G, Boatto G (2013) Validated liquid chromatography–mass spectrometry method for the quantitation of N-substituted derivatives of 3,4-methylenedioxyamphetamine in rat urine. Forensic Toxicol 31:204–211
Nakazono Y, Tsujikawa K, Kuwayama K, Kanamori T, Iwata YT, Miyamoto K, Kasuya F, Inoue H (2013) Differentiation of regioisomeric fluoroamphetamine analogs by gas chromatography–mass spectrometry and liquid chromatography–tandem mass spectrometry. Forensic Toxicol 31:241–250
Zaitsu K, Miyagawa H, Sakamoto Y, Matsuta S, Tsuboi K, Nishioka H, Katagi M, Sato T, Tatsuno M, Tsuchihashi H, Suzuki K, Ishii A (2013) Mass spectrometric differentiation of the isomers of mono-methoxyethylamphetamines and mono-methoxydimethylamphetamines by GC–EI–MS–MS. Forensic Toxicol 31:292–300
Shiotsuki T, Yoshitome K, Moriya F (2012) Use of on-site immunoassay devices to screen urine absorbed in disposable diapers for methamphetamine: a preliminary study with artificial urine. Forensic Toxicol 30:162–167
McLaughlin P, Pounder D, Maskell P, Osselton D (2013) Real-time near-body drug screening during autopsy I: use of the Randox biochip drugs of abuse DOA I and DOA II immunoassays. Forensic Toxicol 31:113–118
Nieddu M, Trignano C, Burrai L, Pirisi MA, Boatto G (2013) Cross-reactivities of 41 new amphetamine-designer drugs to EMIT® immunoassays. Forensic Toxicol 31:133–137
Nakanishi K, Miki A, Zaitsu K, Kamata H, Shima N, Kamata T, Katagi M, Tatsuno M, Tsuchihashi H, Suzuki K (2012) Cross-reactivities of various phenethylamine-type designer drugs to immunoassays for amphetamines, with special attention to the evaluation of the one-step urine drug test Instant-View™, and the Emit® assays for use in drug enforcement. Forensic Sci Int 217:174–181
Kerrigan S, Mellon MB, Banuelos S, Arndt C (2011) Evaluation of commercial enzyme-linked immunosorbent assays to identify psychedelic phenethylamines. J Anal Toxicol 35(7):444–451
Decree of the Italian Republic President, no. 309/1990. “Testo unico delle leggi in materia di disciplina degli stupefacenti e sostanze psicotrope, prevenzione, cura e riabilitazione dei relativi stati di tossicodipendenza”.–“Law for the discipline of abuse drugs and psychotropic substances, prevention, treatment and rehabilitation of drug addiction -Italian”. (Italian Official Gazette No. 25/1990, Suppl.Ord. No. 67)
Italian Decree “Provvedimento no. 99/cu del 30/10/2007, Intesa, ai sensi dell’articolo 8, comma 6, della legge 5 giugno 2003, n. 131, in materia di accertamento di assenza di tossicodipendenza–Italian”. “Measure no. 99/cu del 30/10/2007, Agreement for the assessment of a drug addiction status, according to the article 8, comma 6, of the law n. 131 of 05/06/2003”. (Italian Official Gazette No. 266/2007)
Shulgin A, Shulgin A (1991) Pihkal, a chemical love story. Transform Press, Berkeley
Package insert. SureStep™ one-step test strip (urine) for AMP, MET and MDMA; Alere Srl, Milan, Italy
Package insert. InstAlert™ Multi-drug one step screen test panel (urine) for COC/AMP500/MET500/THC/MTD/MDMA/MOP. Biosigma Srl, Venice, Italy
Package insert. Screen®7 multi-drug one step screen test panel (urine) for COC/AMP500/MET500/THC/MTD/MDMA/MOP. SCREEN ITALIA Srl, Perugia, Italy
Italian Decree Provvedimento no. 178/csr del 18/09/2008. Accordo, ai sensi dell’articolo 8, comma 2 dell’Intesa in materia di accertamento di assenza di tossicodipendenza, perfezionata nella seduta della Conferenza Unificata del 30 ottobre 2007-Italian. Measure no. 178 of the 18/09/2008. Agreement according to the article 8, comma 6, of the Unified Conference of 30/10/2007 about the assessment of a drug addiction status. (Italian Official Gazette No. 236/2008)
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Nieddu, M., Burrai, L., Trignano, C. et al. Cross-reactivities of 39 new amphetamine designer drugs on three abuse drugs urinary screening tests. Forensic Toxicol 32, 132–138 (2014). https://doi.org/10.1007/s11419-013-0198-1
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DOI: https://doi.org/10.1007/s11419-013-0198-1