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Impurity analysis of gentamicin bulk samples by improved liquid chromatography-ion trap mass spectrometry

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Abstract

Several gentamicin bulk samples from different origins were investigated using an LC/MS method. LC equipped with ion trap MS with positive ionization was performed on a Capcell Pak C18 (AQ) column with the mobile phase containing 50 mM trifluoroacetic (TFA) and methanol. Impurities present in batches of gentamicin bulk samples were elucidated and compared according to their fragmentation behavior. In total seventeen impurities present in samples, five impurities were not elucidated and two compounds were identified preliminarily. It was observed that the impurity profiles were different in samples from different origins which indicate necessity in the quality control of gentamicin.

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Authors and Affiliations

  1. Bioengineering College of Chongqing University, Chongqing, 400030, China

    Chao Zheng & BoChu Wang

  2. Laboratorium voor Farmaceutische Analyse, O&N2, Katholieke Universiteit Leuven, Faculteit Farmaceutische Wetenschappen, PB 923, Herestraat 49, B-3000, Leuven, Belgium

    Chao Zheng, MingJuan Wang, Jos Hoogmartens, Ann Van Schepdael & Erwin Adams

  3. National Institute for Control of Pharmaceutical and Biological Products, Beijing, 100050, China

    MingJuan Wang

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  1. Chao Zheng
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  2. BoChu Wang
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  4. Jos Hoogmartens
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  5. Ann Van Schepdael
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  6. Erwin Adams
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Correspondence to BoChu Wang.

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Zheng, C., Wang, B., Wang, M. et al. Impurity analysis of gentamicin bulk samples by improved liquid chromatography-ion trap mass spectrometry. Sci. China Chem. 54, 1518–1528 (2011). https://doi.org/10.1007/s11426-011-4304-0

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  • DOI: https://doi.org/10.1007/s11426-011-4304-0

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