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Experimental research on ectopic osteogenesis of BMP2-derived peptide P24 combined with PLGA copolymers

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Summary

To experimentally evaluate the ectopic osteogenetic capacity of synthesized BMP2-derived peptide P24 combined with poly lactic-co-glycolic acid (PLGA), Wistar rats were divided into two groups: group A, in which BMP2-derived peptide P24/PLGA complex was implanted, and group B which received simple PLGA implant. The complex was respectively implanted into the back muscles of rats. Samples were taken the 1st, 4th, 8th, and the 12th week after the implantation. Their bone formation was detected by X-ray examination, and tissue response was histologically observed. Western blotting was used for the detection of the expression of collagen I (Col-I) and osteopontin (OPN). There was acute inflammation in the tissue around both types of implants at early stage. The cartilage was found around implant areas 4 weeks after the implantation of BMP2-derived peptide p24/PLGA complex, 8 weeks after the implantation, osteoblasts were found, and 12 weeks after the implantation, typical trabecular bone structure was observed. In group B, after 12 weeks, no osteoblasts were found. It is concluded that PLGA is an ideal scaffold material for bone tissue engineering. BMP2-derived peptide can start endochondral ossification and is more effective in inducing ectopic osteogenesis.

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Authors and Affiliations

  1. Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Duan Zhixia  (段智霞), Zheng Qixin  (郑启新), Guo Xiaodong  (郭晓东), Yuan Quan  (袁泉) & Chen Shunguang  (陈顺广)

Authors
  1. Duan Zhixia  (段智霞)
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  2. Zheng Qixin  (郑启新)
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  3. Guo Xiaodong  (郭晓东)
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  4. Yuan Quan  (袁泉)
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  5. Chen Shunguang  (陈顺广)
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Additional information

DUAN Zhixia, female, born in 1967, Associate Professor

This project was supported by grants from the National Natural Sciences Foundation of China (Nos. 30470483 · 30200063, 30170270) and a grant from Chengguang Program for Young Scientists of Wuhan Municipal Government (No. 20045006071-10).

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Duan, Z., Zheng, Q., Guo, X. et al. Experimental research on ectopic osteogenesis of BMP2-derived peptide P24 combined with PLGA copolymers. J. Huazhong Univ. Sc. Technol. 27, 179–182 (2007). https://doi.org/10.1007/s11596-007-0219-6

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  • DOI: https://doi.org/10.1007/s11596-007-0219-6

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