Abstract
An efficient in vitro plant regeneration system was established from callus culture of Scopolia parviflora. Callus was induced from adventitious roots on B5 medium with 0.45–9.04 μM 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro plantlet regeneration was achieved on B5 medium supplemented with 44.38 μM benzyladenine (BA), 3% sucrose, and 0.38% gelrite. Plantlets were transplanted to artificial soil and grown to maturity successfully in a greenhouse. The tropane alkaloid contents in regenerated plants were analyzed using high-performance liquid chromatography (HPLC), and were found to be higher than those of adventitious roots, native growing plants, and acclimated plants. Regenerated plants from organogenic callus cultures produced a greater amount of tropane alkaloids.
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This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund; KRF-2006-521-F00023).
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Kim, Y.D., Min, J.Y., Kim, W.J. et al. High frequency plant regeneration and accumulation of tropane alkaloids in regenerated plants of Scopolia parviflora . In Vitro Cell.Dev.Biol.-Plant 44, 203–208 (2008). https://doi.org/10.1007/s11627-007-9073-2
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DOI: https://doi.org/10.1007/s11627-007-9073-2