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In vitro effects of GA3 on morphogenesis of CIP potato explants and acclimatization of plantlets in field

  • Plant Tissue Culture
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Abstract

Improvement of potato has been accomplished using conventional and non-conventional approaches coupled with numerous tissue culture procedures. The aim of the present study was to assess the efficacy of gibberellic acid (GA3) on the morphogenesis of International Potato Center (CIP) potato explants and acclimatization of plantlets in the field. Nodal segments as an explant source (1–1.5 cm) were isolated from 31 CIP potato plantlets and were inoculated into Murashige and Skoog (MS) medium supplemented with 0.0 (control), 0.1, 0.5, or 1.0 mg L−1of GA3. The variation in growth parameters of the cultivars was then observed. The highest shoot induction occurred in MS medium containing 1.0 mg L−1 GA3 with an increase in the inter-nodal distance between nodes as compared to other treatments. Higher concentration (1.0 mg L−1) of GA3 significantly increased plant height and root length in the treated germplasm however; this concentration was inhibitory to the number of nodes and roots per plant. The number of leaves was significantly increased in plants receiving GA3 treatment at lower concentration (0.1 mg L−1). The 31 CIP genotypes were transplanted to the field and checked for yield quality traits. It was concluded from the results that GA3 had significant effects on morphogenesis and was effective in the acclimatization of CIP potato plantlets in field.

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Authors and Affiliations

  1. Plant Epigenetics and Development Northeast Forestry University, Harbin, China

    Shahid Ali

  2. Department of Plant Sciences, Quaid-I-Azam University, Islamabad, Pakistan

    Naeem Khan

  3. Department of Botany, Hazara University, Mansehra, Pakistan

    Faisal Nouroz

  4. National Agricultural Research Center, Islamabad, Pakistan

    Shazia Erum

  5. COMSATS Institute of Information Technology, Vehari, Pakistan

    Wajid Nasim

  6. Horticultural Sciences Department, IFAS, University of Florida, Gainesville, USA

    Muhammd Adnan Shahid

Authors
  1. Shahid Ali
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  2. Naeem Khan
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  3. Faisal Nouroz
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  4. Shazia Erum
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  5. Wajid Nasim
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  6. Muhammd Adnan Shahid
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Correspondence to Shahid Ali.

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Editor: Zeng-Yu Wang

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Ali, S., Khan, N., Nouroz, F. et al. In vitro effects of GA3 on morphogenesis of CIP potato explants and acclimatization of plantlets in field. In Vitro Cell.Dev.Biol.-Plant 54, 104–111 (2018). https://doi.org/10.1007/s11627-017-9874-x

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  • DOI: https://doi.org/10.1007/s11627-017-9874-x

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