Skip to main content
Log in

Biodiversity of endophytic fungi from seven herbaceous medicinal plants of Malnad region, Western Ghats, southern India

  • Original Paper
  • Published:
Journal of Forestry Research Aims and scope Submit manuscript

Abstract

A total of 3611 fungal isolates were recovered from 4200 leaf segments incubated from 7 medicinal herbs during monsoon, winter and summer seasons. These fungal isolates belonged to teleomorphic Ascomycota (23.5%), anamorphic Ascomycota producing conidiomata (17.4%), anamorphic Ascomycota without conidiomata (46.9%), Zygomycota (1.42%) and sterile forms (10.6%). Chaetomium globosum, Aspergillus niger, Aureobasidium pullulans, Curvularia lunata, Fusarium spp., Penicillium spp., Pestalotiopsis spp., Trichoderma viridae, Cladosporium cladosporioides, were frequently isolated from more than one host plant. The number of endophytic isolates was higher in winter than in monsoon and summer seasons.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Agrios GN. 1997. Plant Pathology, 4th edition. London: Academic Press, p. 635.

    Google Scholar 

  • Aly AH, Debbab A, Kjer J, Proksch P. 2010. Fungal endophytes from higher plants: a prolific source of phytochemicals and other bioactive nature products. Fungal Diversity, 41: 1–16.

    Article  Google Scholar 

  • Arnold AE. 2008. Endophytic Fungi: Hidden Components of Tropical Community Ecology. In: Schnitzer S, Carson W. (eds), Tropical Forest Community Ecology. London: Blackwell Scientific, pp. 254–271.

    Google Scholar 

  • Arnold AE, Lutzoni F. 2007. Diversity and host range of foliar fungal endophytes: are tropical leaves biodiversity hotspots? Ecology, 88: 541–549.

    Article  PubMed  Google Scholar 

  • Arnold AE, Maynard Z, Gilbert GS, Coley PD, Kursar TA. 2000. Are tropical fungal endophytes hyper diverse? Ecological Letters, 3: 267–274.

    Article  Google Scholar 

  • Bacon CW, White JF. 2000. Microbial Endophytes. New York: Marcel Decker INC, pp. 237–261.

    Google Scholar 

  • Barnett HL, Hunter BB. 1998. Illustrated Genera of Imperfect Fungi, 4th edn. New York: Prentice-Hall, Inc., p. 218.

    Google Scholar 

  • Bills GF. 1996. Isolation and analysis of endophytic fungal communities from woody plants. In: Redlin S, Carris LM. (eds), Systematics, Ecology and Evolution of Endophytic Fungi in Grasses and Woody Plants. St Paul, MN: APS Press, pp. 31–65.

    Google Scholar 

  • Clay K. 1988. Fungal endophytes of grasses: a defensive mutualism between plants and fungi. Ecology, 69: 10–16.

    Article  Google Scholar 

  • Clay K, Holah J. 1999. Fungal endophyte symbiosis and plant diversity in successional fields. Science, 285: 1742–1744.

    Article  CAS  PubMed  Google Scholar 

  • Ernst M, Mendgen KW, Wirsel SGR. 2003. Endophytic fungal mutualists: seed-borne Stagonospora spp. enhances reed biomass production in axenic microcosms. Molecular Plant Microbe Interactions, 16: 580–587.

    Article  CAS  PubMed  Google Scholar 

  • Fisher PJ, Anson AE, Petrini O. 1986. Fungal endophytes in Ulexeuropaeus and Ulexgallii. Transactions of the British Mycological Society, 86: 153–156.

    Article  Google Scholar 

  • Fröhlich J, Hyde KD. 1999. Biodiversity of palm fungi in the tropics: are global fungal diversity estimates realistic? Biodiversity and Conservation, 8: 1742–1744.

    Article  Google Scholar 

  • Gamboa MA, Laureano P, Bayman P. 2002. Measuring diversity of endophytic fungi in leaf fragments: Does size matter? Mycopathologia, 156: 41–45.

    Article  PubMed  Google Scholar 

  • Gibbons JD. 1976. Non-parametric methods for quantitative analysis. New York: Holt, Rine hart and Winston, p. 463.

    Google Scholar 

  • Göre ME, Bucak C. 2007. Geographical and seasonal influence on the distribution of fungal endophytes in Laurusnobilis. Forest Pathology, 37: 281–288.

    Article  Google Scholar 

  • Gunatilaka AAL. 2006. Natural products from plant-associated microorganisms: distribution, structural diversity, bioactivity, and implications of their occurrence. Journal of Natural Products, 69: 509–526.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Guo LD, Huang GR, Wang Y. 2003. Molecular identification of white morphotype strains of endophytic fungi from Pinustabulaeformis. Mycological Research, 107: 680–688.

    Article  CAS  PubMed  Google Scholar 

  • Hawksworth DL. 2001. The magnitude of fungal diversity: the 1.5 million species estimate revisited. Mycological Research, 105: 1422–1432.

    Article  Google Scholar 

  • Huang WY, Cai YZ, Hyde KD, Corke H, Sun M. 2008. Biodiversity of endophytic fungi associated with 29 traditional Chinese medicinal plants. Fungal Diversity, 33: 61–75.

    Google Scholar 

  • Hyde KD, Bussaban B, Paulus B, Crous PW, Lee S, Mckenzie EHC, Photita W, Lumyong S. 2007. Biodiversity of saprobic fungi. Biodiversity and Conservation, 16: 17–35.

    Article  Google Scholar 

  • Jeewon R, Hyde KD. 2006. Diversity and detection of fungi from environmental samples: traditional versus molecular approaches. In: Varma A, Oelmuller R. (eds), advanced techniques in Soil microbiology, Soil biology series. BerlinHeidelberg: Springer-Verlag press, pp. 1–11.

    Google Scholar 

  • Koide K, Osono T, Takeda H. 2005. Colonization and lignin decomposition of Camellia japonica leaf litter by endophytic fungi. Mycoscience, 46: 280–286.

    Article  Google Scholar 

  • Krishnamurthy YL, Shankar Naik B, Shashikala J. 2008. Fungal communities in herbaceous medicinal plants, Malnad region, Southern India. Microbes and Environment, 23(1): 24–28.

    Article  Google Scholar 

  • Krishnamurthy YL, Shashikala J, Shankar Naik B. 2009. Diversity and seasonal variation of endophytic fungal communities associated with some medicinal trees of Western Ghats, Southern India. Sydowia, 61(2): 255–266.

    Google Scholar 

  • Kusari S, Lamshöft M, Zühlke S, Spiteller M. 2008. An endophytic fungus from Hypericumperforatum that produces hypericin. J Nat Prod, 71: 159–162.

    Article  CAS  PubMed  Google Scholar 

  • Lodge DJ, Fisher PJ, Sutton BC. 1996. Endophytic fungi of Manilkarabidentataleave in Puerto Rico. Mycologia, 88: 733–738.

    Article  Google Scholar 

  • Márquez LM, Redman RS, Rodriguez RJ, Roossinck MJ. 2007. A virus in a fungus in a plant: three-way symbiosis required for thermal tolerance. Science, 315: 513–515.

    Article  PubMed  Google Scholar 

  • Pelaez F. 2005. Biological activities of fungal metabolites. In: An Z. (ed). Handbook of industrial mycology. New York: Marcel Dekker, p. 49–92.

    Google Scholar 

  • Petrini O. 1986. Taxonomy of endophytic fungi of aerial plant tissues. Microbiology of Plant Microbe Interactions, 16: 580–587.

    Google Scholar 

  • Redman RS, Sheehan KB, Stout RG, Rodrigues RJ, Henson JM. 2002. Thermo tolerance conferred to plant host and fungal endophyte during mutualistic symbiosis. Science, 298: 1581.

    Article  CAS  PubMed  Google Scholar 

  • Rodrigues KF, Costa GL, Carvalho MP, Epifanio RDA. 2005. Evaluation of extracts produced by some tropical fungi as potential cholinesterase inhibitors. World Journal of Microbiology and Biotechnology, 21: 1617–1621.

    Article  Google Scholar 

  • Rodriguez RJ, White JF, Arnold AE, Redman RS. 2009. Fungal endophytes: diversity and functional roles. New Phytologist, 182: 314–330.

    Article  CAS  PubMed  Google Scholar 

  • Rudgers JA, Clay K. 2007. Endophyte symbiosis with tall fescue: how strong are the impacts on communities and ecosystems? Fungal Biology Reviews, 21: 107–124.

    Article  Google Scholar 

  • Schulthess FM, Faeth SH. 1998. Distribution, abundances and association of the endophytic fungal community of Arizona fescue (Festucaarizonica Vasey). Mycology, 90: 569–578.

    Article  Google Scholar 

  • Schulz B, Boyle C. 2005. The endophytic continuum. Mycological Research, 109: 661–686.

    Article  PubMed  Google Scholar 

  • Schulz B, Wanke U, Draenger S, Aust HJ. 1993. Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycological research, 97: 1447–1450.

    Article  Google Scholar 

  • Shankar Naik B, Shashikala J, Krishnamurthy YL. 2008. Diversity of endophytic fungal communities in shrubby medicinal plants of Western Ghat region, Southern India. Fungal Ecology, 1: 89–93.

    Article  Google Scholar 

  • Shankar Naik B, Shashikala J, Krishnamurthy YL. 2006. Study on diversity of endophytic communities from rice (Oryza sativa L.) and their antagonistic activities in vitro. Microbiological Research, 3: 290–296.

    Google Scholar 

  • Shentu XP, Chen LZ, Yu XP. 2007. Anti-fungi activities and cultural characteristics of gingko endophytic fungus No. 1028. Acta Phytophyl Sin, 34: 147–152.

    CAS  Google Scholar 

  • StrobelGA, Daisy B. 2003. Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews, 67: 491–502.

    Article  Google Scholar 

  • Strobel GB, Daisy U, Castillo Harper JJ. 2004. Natural products from endophytic microorganisms. Natural Products, 67: 257–268.

    Article  CAS  Google Scholar 

  • Subramanian CV. 1983. Hyphomycetes, Taxonomy and Biology. New York: Academic Press, p. 450.

    Google Scholar 

  • Suryanarayanan TS, Murali TS, Venkatesan G. 2003. Endophytic fungal communities in leaves of tropical forest trees: diversity and distribution patterns. Current Science, 85: 489–493.

    Google Scholar 

  • Sutton BC. 1980. The Coelomycetes. London, England: Kew, Commonwealth Mycological Institute, p. 696.

    Google Scholar 

  • White JF Jr, Col GT. 1985. Endophyte host associations in forage grasses. I. Distribution of Fungal endophytes in some species of Lolium and Festuca. Mycologia, 77: 323–327.

    Google Scholar 

  • Wilson D. 2000. Ecology of woody plant endophytes. In: Bacon CW, White JF. (eds), Microbial endophytes. New York, Basel: Marcel Dekker, pp 389–420.

    Google Scholar 

  • Wilson D, Carroll GC. 1994. Infection studies of Disculaquercina and endophyte of Quercusgarryana. Mycologia, 86: 635–647.

    Article  Google Scholar 

  • Yu W, Dai CC. 2011. Endophytes: a potential resource for biosynthesis, biotransformation, and biodegradation. Annals of Microbiology, 61: 207–215.

    Article  Google Scholar 

  • Zamora P, Martínez-Ruiz C, Diez JJ. 2008. Fungi in needles and twigs of pine plantations from northern Spain. Fungal Diversity, 30: 171–184.

    Google Scholar 

  • Zar HJ. 2004. Biostatistical analysis, 4th edn. Delhi: Pearson Education Pvt. Ltd, p. 663.

    Google Scholar 

  • Zhou L, Zhao J, Xu L, Huang Y, Ma Z, Wang J, Jiang W. 2009. Antimicrobial compounds produced by plant endophytic fungi. In: De Costa P, Bezerra P. (eds), Fungicides: Chemistry, Environmental Impact and Health Effects. New York: Nova Science Publishers, 91:116–119.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to B. Shankar Naik.

Additional information

Project funding: The study was supported by University Grants Commission, India.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shankar Naik, B., Krishnappa, M. & Krishnamurthy, Y.L. Biodiversity of endophytic fungi from seven herbaceous medicinal plants of Malnad region, Western Ghats, southern India. Journal of Forestry Research 25, 707–711 (2014). https://doi.org/10.1007/s11676-014-0511-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11676-014-0511-9

Keywords

Navigation