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Determination of Serum Oxidative Stress, Antioxidant Capacity and Protein Profiles in Dogs Naturally Infected with Ehrlichia canis

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Abstract

Purpose

Canine ehrlichiosis is an important tick-borne disease of dogs worldwide. In the present study, we aimed to determine the serum total antioxidant capacity (TAC), malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase, (SOD), glutathione peroxidase (GSH-Px), adenosine deaminase (ADA) activity and serum protein profiles in dogs affected with naturally acquired ehrlichiosis.

Methods

The animal materials had been consisted of ten dogs naturally infected with Ehrlichia canis, and ten controls negative for Ehrlichia canis. TAC, MDA, NO, SOD, GSH-Px, ADA activity and TP, ALB, GLOB levels were measured in sera of the animals. The serum protein concentrations were measured by autoanalyzer. The electrophoretic profiles of serum total protein were determined by native polyacrylamide gel electrophoresis (Native-PAGE).

Results

In dogs with ehrlichiosis, decreased TAC (P < 0.05) and GSH-Px (P > 0.05) levels were determined. However, NO (P > 0.05), SOD (P < 0.05), ADA (P > 0.05), MDA (P > 0.05), TP (P < 0.05) and GLO (P < 0.05) levels were found as increased in the Ehrlichia positive dogs. ALB levels were decreased without a statistical significance (P > 0.05). ALB, α1 and β2 globulin strip densities were found as decreased in native-PAGE, while β1 and γ globulin strip densities were significantly increased in the E. canis positive group when compared to the control.

Conclusion

It was determined that the oxidative stress decreased high antioxidant activity in dogs naturally infected with E. canis, and consequently, pro-oxidant and antioxidant defense and serum protein profiles were affected. It was thought that antioxidant supplementation could be beneficial to the treatment of the disease.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Atakum, 55200, Samsun, Turkey

    Gülay Çiftci & Sena Çenesiz

  2. Department of Internal Medicine, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Atakum, 55200, Samsun, Turkey

    Didem Pekmezci & Murat Güzel

  3. Department of Internal Medicine, Faculty of Veterinary Medicine, University of Adnan Menderes, 09016, Isıklı, Aydın, Turkey

    Kerem Ural

  4. Department of Parasitology, Faculty of Veterinary Medicine, University of Adnan Menderes, 09016, Işıklı, Aydın, Turkey

    Nuran Aysul

  5. Department of Biochemistry, Faculty of Veterinary Medicine, University of Mustafa Kemal University, Hatay, Turkey

    Filiz Kazak

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  1. Gülay Çiftci
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Correspondence to Gülay Çiftci.

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The animal procedures were approved by the Local Ethical Committee of University of Adnan Menderes for Animal Studies (certificate no: B.30.2.adü.0.00.00.00.-0.50.0.4.-2010-320 077). The animals were treated according to the Animal Care and Use Regulation as described by European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes.

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Çiftci, G., Pekmezci, D., Güzel, M. et al. Determination of Serum Oxidative Stress, Antioxidant Capacity and Protein Profiles in Dogs Naturally Infected with Ehrlichia canis. Acta Parasit. 66, 1341–1348 (2021). https://doi.org/10.1007/s11686-021-00411-6

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