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Effect of temperature stress on the endogenous cytokinin content in Arabidopsis thaliana (L.) Heynh plants

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Abstract

The levels of three endogenous cytokinin equivalents: zeatin (Z), iso-pentenyladenine (iP) and dihydrozeatin (dZ) in two Arabidopsis thaliana (L.) Heynh genotypes — wild type (wt) and ethylene-insensitive mutant (eti5), were compared using enzyme immunoassay (ELISA). Cytokinin content was measured after exposure to low (4 °C for 24 h in darkness) or high temperature (38 °C for 24 h in darkness). Measurements were performed immediately and 24, 48 and 120 h after treatments. It was found that at normal growth conditions eti5 plants contained more endogenous cytokinins compared to the wild type. At both temperature treatments mutant plants had decreased total cytokinin levels. Wild-type plants treated with high temperature (HT) exhibited reduced total cytokinins (with the exception of rates at 48 h), while low temperature (LT) treatment resulted in elevated total amount of the studied equivalents (except at 24 h). The obtained results suggested that HT had greater effect on cytokinin levels than LT since it caused more profound changes in the total content. We assume that this was due to the natural chilling tolerance of Arabidopsis plants.

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Abbreviations

dZ:

dihydrozeatn

dZR:

dihydrozeatin riboside

ELISA:

enzyme linked immunosorbent assay

HT:

high temperature

iP:

iso-pentenyladenine

iPA:

iso-pentenyladenosine

LT:

low temperature

SE:

standard error

wt:

wild type

Z:

zeatin

ZR:

zeatin riboside

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Authors and Affiliations

  1. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 21, 1113, Sofia, Bulgaria

    Dessislava Todorova, Irina Vaseva-Gemisheva, Vera Alexieva & Emanuil Karanov

  2. Institute of Biological Sciences, University of Wales, Edward Llwyd Building, SY23 3DA, Aberystwyth, Ceredigion, UK

    Aileen Smith & Michael Hall

  3. Department of Plant Physiology, Faculty of Biology, Sofia University, 8 Blvd. Dragan Tsankov, 1421, Sofia, Bulgaria

    Todor Genkov

Authors
  1. Dessislava Todorova
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  2. Todor Genkov
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  3. Irina Vaseva-Gemisheva
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  4. Vera Alexieva
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  5. Emanuil Karanov
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  6. Aileen Smith
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  7. Michael Hall
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Todorova, D., Genkov, T., Vaseva-Gemisheva, I. et al. Effect of temperature stress on the endogenous cytokinin content in Arabidopsis thaliana (L.) Heynh plants. Acta Physiol Plant 27, 13–18 (2005). https://doi.org/10.1007/s11738-005-0031-5

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  • DOI: https://doi.org/10.1007/s11738-005-0031-5

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