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The effect of etiolation and shading of stock plants on rhizogenesis in stem cuttings of Cotinus coggygria

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Abstract

In order to improve vegetative propagation of a difficult to root Cotinus coggygria the stock plants were subjected to: etiolation, shading and spraying with IBA, combined with the application of two commercially available rooting powders. The IBA treatment was more suitable for rooting of C. coggygria cuttings than the NAA application and it enhanced rhizogenesis regardless of the form of auxin application (foliar application to a stock plant or a rooting powder used directly on cuttings) and the amount of light provided to stock plants. Etiolation did not improve rhizogenesis in stem cuttings, however, reduction of light intensity by 50% and 96% of the ambient prior to harvest of cuttings affected rooting positively. Positive effects of shading can be ascribed to changes in shoot anatomy, i.e. a weaker sclerenchyma development. Synergistic effect of shading and foliar auxin application can result from the increase in leaf blade area and/or thinner lower epiderm. Enhanced rooting in cuttings from shoots grown out under reduced light intensity was accompanied by decrease in the contents of total soluble sugars, soluble proteins and free ABA and by increase in total chlorophyll, free amino acids, polyphenolic acids and free IAA contents.

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References

  • Ahmed K., Tartaura H., 2002. Effect of abscisic acid on endogenous IAA, auxin protector levels and peroxidase activity during adventitious root initiation in Vigna radiata cuttings. Acta Physiol. Plant. 23: 149–156.

    Google Scholar 

  • Alia and P.P. Saradahi, 1991. Proline accumulation under heavy metal stress. J. Plant Physiol. 138: 554–558.

    CAS  Google Scholar 

  • Bassuk N.L., Howard B.H., 1981. A positive correlation between endogenous root inducing cofactor activity in vacuum extracted sap and seasonal changes in rooting of M 26 apple cuttings. J. Hort. Sci. 56: 301–312.

    Google Scholar 

  • Bertram L., 1992. The physiological background for regulation of adventitious root formation by the irradiance to stock plants: a hypothesis. Acta Hort. 314: 291–299.

    Google Scholar 

  • Bukova M.J., 1972. Foliar penetration of plant growth substances with special reference to fruit trees. Acta Hort. 34: 69–77.

    Google Scholar 

  • Caesar J.C., 1990. Effect of simulated shade-light quality on stem anatomy of Pinus contorta seedlings. IAWA-Bulletin 11: 2, 120.

    Google Scholar 

  • Chen K. H., Miller A. N., Paterson G. W., 1998. A rapid and simple procedure for purification of indole-3-acetic acid prior to GC-SIM-MS analysis. Plant Physiol. 80: 822–825.

    Google Scholar 

  • Chowdhury P.K., Thangaraj M., Jayapragasm M., 1994. Biochemical changes in low irradiance tolerant and susceptible rice cultivars. Biol. Plant. 36: 237–242.

    Article  CAS  Google Scholar 

  • De Klerk G.J., Ter Brugge J., Marinova S. 1997. Effectiveness of indoleacetic acid, indolebutyric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork 9’. Plant Cell Tissue and Organ Culture 49: 39–44.

    Article  Google Scholar 

  • Doud S.L., Carlson R.F., 1977. Effects of etiolation, stem anatomy, and starch reserves on root initiation of layered Malus clones. J. Amer. Soc. Hort. Sci. 102: 487–491.

    CAS  Google Scholar 

  • Dubois M., Gilles K.A., Hamilton J.K., 1956. Colorimetric metod for determination of sugars and related substances. Anal. Chem. 28: 350–356.

    Article  CAS  Google Scholar 

  • Dunn D.E., 1999. Timing and auxin concentration affects Cotinus coggygria ‘Royal Purple’ rooting. Comb. Proc. Int. Plant Prop. Soc. 49: 510–513.

    Google Scholar 

  • Eliasson L., 1980. Interaction of light and auxin in regulation of rooting in pea steam cuttings. Physiol. Plant. 48: 78–82.

    Article  CAS  Google Scholar 

  • Foong T.W., Barnes M.F., 1981. Rooting “cofactors” in rhododendrons: the fractionation and activity of components from an easy-to-root and difficult-to-root variety. Bioch. Physiol. Pflanzen. 176: 507–523.

    CAS  Google Scholar 

  • Frolich E.F., 1961. Etiolation and the rooting of cutings. Calif. Avocado Soc. Yearbook 34: 136–138.

    Google Scholar 

  • Grafi G., Shomer-Ilan A., Waisel Y., 1994. Effects of fermented cow-manure on rooting of mung bean cuttings: The role of nutrients and of abscisic acid. J. Plant Nutrition 17: 401–413.

    Google Scholar 

  • Harrison-Murray R.S., Howard B.H., 1982. Effects of prior etiolation on adventitious rooting of apple cuttings. Int. Soc. Hort. Sci. 21st Int. Hortic. Cong., Hamburg. Vol 2: 1281.

    Google Scholar 

  • Hartmann H.T., Kester D.E., Davies F.T., Geneve R.L., 1997. Plant propagation-Principles and Practices, 6th ed., Prentice Hall, Upper Saddle River, p. 276–385, 667–725.

    Google Scholar 

  • Henriksson J., Haukioja E., Ossipov V., 2003. Effects of host shading on consumption and growth of the geometrid Epirrita autumnata: Interactive roles of water, primary and secondary compounds. Dept. of Biology, Univ. of Turku, Finland, Oikos 103: 3–16.

    CAS  Google Scholar 

  • Herman D.E., Hess C.E., 1963. The effect of etiolation upon the rooting of cuttings. Comb. Proc. Inter. Plant Prop. Soc. 13: 42–62.

    Google Scholar 

  • Howard B.H., 1984. Plant propagation. Rep. E. Malling Res. Stn. For 1983: 77–91.

    Google Scholar 

  • Inskeep W.P., Bloom P.R., 1985. Extinction coefficients of chlorophyll a and b in N,N-dimethylformamide and 80% acetone. Plant Physiol. 77: 483–485.

    PubMed  CAS  Google Scholar 

  • Lowry J. O. H., Rosebrough N. J., Farr A. L., Randall R. J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–267.

    PubMed  CAS  Google Scholar 

  • Kopcewicz J., Lewak S., 2002. Podstawy fizjologii ro lin. [Bases of Plant Physiology] PWN, Warszawa: 330–348, 613–678.

    Google Scholar 

  • Macdonald B., 1989. Practical woody plant propagation for nursery growers. Timber Press, Portland, Oregon, p. 353–380.

    Google Scholar 

  • Marczy ski S., Joustra M. K., 1993. Influence of day length and irradiance on growth of the stock plants and subsequent rooting of cuttings of Betula utilis D. Don and Corylus maxima Mil. cultivar ‘Purpurea’. Sci. Hort. 5: 291–301.

    Article  Google Scholar 

  • Maynard B.K., Bassuk N.L., 1985. Etiolation as a tool for rooting cuttings of difficult-to-root woody plants. Proc. Int. Plant Prop. Soc. 35: 487–495.

    Google Scholar 

  • Maynard B.K., Bassuk N.L., 1992. Stock plant etiolation, shading, banding effects on cutting propagation of Carpinus betulus. J. Amer. Soc. Hort. Sci. 117: 740–744.

    Google Scholar 

  • Maynard B.K., Bassuk N.L., 1996. Effects of stock plant etiolation, shading, banding and shoot development on histology and cutting propagation of Carpinus betulus L. ‘Fastigiata’. J. Amer. Soc. Hort. Sci. 121: 853–860.

    Google Scholar 

  • Morgan R., Porath D., 1980. Chlorophyll determination in intact tissues using N,N-Dimethylformamide. Plant Physiol. 65: 478–479.

    Article  Google Scholar 

  • Nicolini E., Chanson B., Bonne F., 2001. Stem growth and epicormic branch formation in understorey beech trees (Fagus sylvatica L.). Ann. Bot. 87: 373–750.

    Article  Google Scholar 

  • Pacholczak A., Szydło W., Łukaszewska A., 2005. Effectiveness of foliar auxin application to stock plants in rooting of stem cuttings of ornamental shrubs. Propagation of Ornamental Plants 5(2): 100–106.

    Google Scholar 

  • Polish Norm PN-91/R-87019, 2002. Pharmacopoea Polonica VI:895–897. ed. Tow. Farmaceutyczne, Warszawa (in Polish).

    Google Scholar 

  • Polikarpowa F.Y., 1971. [The role of etiolation by softwood cuttings]. Sbornik Naucznych rabot WNIJ Sadostvo. 16: 106–112 (in Russian).

    Google Scholar 

  • Rosa L.S., Sa T.D., Carvalho C.J.R., 1998. Ecophysiological and morphological responses of rosewood (Aniba rosaeodora Ducke) to different levels of shading in nursery conditions. Boletim da Faculdade de Ciencias Agrarias do Para, Brazil 30: 119–132.

    Google Scholar 

  • Rosen H., 1957. A modified ninhydrin colourimetric analysis for amino acids. Archives of Biochemistry and Biophysics 67: 10–15.

    Article  PubMed  CAS  Google Scholar 

  • Roncatto G., Goncalves E.D., Dutra L.F., Kersten E., 1999. Influence of shading plants and indolebutyric acid on rooting of cuttings of Valencia orange cultivar (Citrus sinensis L.). Revista Cientifica Rural. 4: 60–65.

    Google Scholar 

  • Song Ch.Y., Song N.H., Shin D.G., 1995. Effect of shade and forcing date on growth and flowering of potted Rhododendron obtusum. J. Korean Soc. For Hort. Sci. 36: 641–648.

    Google Scholar 

  • Stanisławski J.J., 1977. [Role of indoleacetic acid in plant growth] (In Polish) Wiadomo ci Botaniczne 21: 43–59. (in Polish)

    Google Scholar 

  • Szydło W., Marczy ski S., 1998. [Effect of foliar auxin application on propagation of ornamental shrubs]—Conference: Propagation of Ornamental Plants, Institute of Pomology and Floriculture, Skierniewice: 33–38. (in Polish)

  • Tanaka T., Matsumoto Y., 2002. Changes of leaf anatomy in 10 deciduous broad-leaved tree species according to change in growth light conditions. Jap. J. Ecol. 52: 323–329 (abstract).

    Google Scholar 

  • Wareing P.F., Philips I.D.J., 1985. [Growth and differentiation in plants] PWN. Warszawa: 204–251. (in Polish).

    Google Scholar 

  • Westwood M. N., Batjer L. P., 1973. The role of growth regulators in rooting. Acta Hort. 34: 89–92.

    Google Scholar 

  • Zaczek J.J., Heuser Ch.W., Stelner K.C., 1997. Light levels and hormone effects during the rooting of selected tree taxa. Comb. Proc. Int. Plant Prop. Soc. 47: 623–631.

    Google Scholar 

  • Zimmerman P. W., Wilcoxon F., 1935. Several chemical growth substances which cause initiation of roots and other responses in plants. Contr. Boyce Thompson Inst. 7: 209–229.

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Ornamental Plants, Faculty of Horticulture and Landscape Architecture, Warsaw Agricultural University, Nowoursynowska 159, 02-787, Warszawa, Poland

    Andrzej Pacholczak, Wiesław Szydło & Aleksandra Łukaszewska

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  1. Andrzej Pacholczak
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  2. Wiesław Szydło
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  3. Aleksandra Łukaszewska
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Pacholczak, A., Szydło, W. & Łukaszewska, A. The effect of etiolation and shading of stock plants on rhizogenesis in stem cuttings of Cotinus coggygria . Acta Physiol Plant 27, 417–428 (2005). https://doi.org/10.1007/s11738-005-0046-y

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  • DOI: https://doi.org/10.1007/s11738-005-0046-y

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