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Plant regeneration via somatic embryogenesis in Pinus kesiya (Rolye ex. Gord.) influenced by triacontanol

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Abstract

Embryogenic cultures were initiated and established for the first time in 3 different genotypes of Pinus kesiya using mature zygotic embryos and triacontanol. Mature zygotic embryos produced white-mucilaginous embryogenic callus when cultured on half strength MSG (Becwar et al. 1990) basal medium supplemented with 90 mM maltose, 2.0 g l−1 Gellan gum, 9.0 M 2, 4-D and 10 g l−1triacontanol. On subculture of such embryogenic callus on the maintenance medium (II) containing 2.0 M 2,4-D and 2.0 g l−1 triacontanol induced cleavage polyembryogenesis with proembryos. The percentage of somatic embryogenesis was not similar in all the three genotypes. The highest percentage of somatic embryogenesis (88.5 %) was recorded in PK04 genotype. Somatic embryos were successfully germinated on half strength MSG basal medium without growth regulators. Somatic seedlings showed fast growth and a survival rate of 95%. This work for the first time reveals that triacontanol can be used as an effective growth regulator for inducing somatic embryogenesis in conifers.

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Abbreviations

ABA:

Abscisic acid

2,4-D:

dichlorophenoxy acetic acid

I:

initiation medium

II:

maintenance medium

III:

maturation medium

IV:

germination medium

TRIA:

triacontanol

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Authors and Affiliations

  1. Division of Plant Biotechnology, Department of Botany, Karnatak University, Pavate Nagar, Dharwad, 580003, Karnataka state, India

    Ravindra B. Malabadi, Gangadhar S. Mulgund & K. Nataraja

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  1. Ravindra B. Malabadi
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  2. Gangadhar S. Mulgund
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  3. K. Nataraja
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Correspondence to Ravindra B. Malabadi.

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Malabadi, R.B., Gangadhar, S.M. & Nataraja, K. Plant regeneration via somatic embryogenesis in Pinus kesiya (Rolye ex. Gord.) influenced by triacontanol. Acta Physiol Plant 27, 531–537 (2005). https://doi.org/10.1007/s11738-005-0059-6

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  • DOI: https://doi.org/10.1007/s11738-005-0059-6

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