Skip to main content
SpringerLink
Log in

Microspore culture of small grain cereals

  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Androgenesis of wheat, rice and triticale was studied in isolated microspore culture. It is the first publication which studies microspore culture reaction of Hungarian rice varieties. The effect of different basic media, lack and absence of growth regulators in culture media were tested on important parameters of microspore culture. Direct embryogenesis was observed in microspore culture of wheat and triticale genotypes. In the case of rice, calli were induced in isolated rice microspore culture and haploid rice plantlets were regenerated via organogenesis.

In wheat, the effect of basic media (W14, A2, CHB3, P4-m) was compared and among them the W14, and A2 had a superior effect on embryo production and albino and green plantlet regeneration. In rice the C, CHB3 and MSm media were tested in microspore culture and the significantly highest numbers of calli were achieved by using C and CHB3 media depending on the genotypes. The lack of exogenous growth regulators was observed in isolated microspore culture of triticale and rice. Growth regulator-free medium had a positive effect on embryo production and plant regeneration of triticale genotypes, whereas in rice microspore culture multicellular structures did not continue their division without growth regulators from the third week of microspore culture. Developing of microspore-origin calli was maintained by supplement of 2,4-D and Kinetin combination in the microspore culture medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Balatero C. H., Darvey N. L., Luckett D. J. 1995. Genetic analysis of anther-culture response in 6x triticale. Theor. Appl. Genet. 90: 279–284.

    Article  Google Scholar 

  • Bernard S. 1980. In vitro androgenesis in hexaploid triticale: determination of physical conditions increasing embryoid and green plant production. Z. Pflanzenzuchtg. 85: 308–321.

    Google Scholar 

  • Blakeslee A., Belling J., Farnham M. E., Bergner A. D. 1922. A haploid mutant in Datura stramonium. Science 55: 646–647.

    Article  PubMed  Google Scholar 

  • Charmet G., Bernard S. 1984. Diallel analysis of androgenetic plant production in hexaploid triticale (× Triticosecale, Wittmack). Theor. Appl. Genet. 69: 55–61.

    Article  Google Scholar 

  • Chen Y., Wang R. F., Tian W. Z. 1980. Studies on pollen culture in vitro and induction of plantlets in Oryza Sativa Subsp. Keng. Acta Genet. Sin. 7: 46–54 (in Chinese).

    Google Scholar 

  • Cho M. S., Zapata F. J. 1988. Callus formation and plant regeneration in isolated pollen culture of rice (Oryza sativa L. cv. Taipei 309) Plant Sci. 58: 239–244.

    Article  Google Scholar 

  • Chu C. C., Hill R. D., Brule-Babel A. L. 1990. High frequency of pollen embryoid formation and plant regeneration in Triticum aestivum L. on monosaccharide containing media. Plant Sci. 66: 255–262

    Article  CAS  Google Scholar 

  • Datta S. K., Wenzel G. 1987. Isolated microspore derived plant formation via embryogenesis of Triticum aestivum L. Plant Sci. 48: 49–54.

    Article  Google Scholar 

  • Gland-Zwerger A., Javornik B., Bohanec B., Kreft I. 1994. Production of doubled haploid lines through anther culture in barley and triticale. In: Proceed. International Colloquium on Impact of Plant Biotechnology on Agriculture, ed. by Javornik B., Bohanec B. December 5–7, Rogla, Slovenia: 9–14.

    Google Scholar 

  • Heszky L. E., Simon I. K. 1992. “Dáma”, the First Plant Variety of Biotechnology Origining from Hungary, Registered in 1992. Hung. Agric. Res. 1: 30–32.

    Google Scholar 

  • Indrianto A., Barinova I., Touraev A., Heberle-Bors E. 2001. Tracking individual wheat microspores in vitro: identification of embryogenic microspores and body axis formation in the embryo. Planta 212: 163–174.

    Article  PubMed  CAS  Google Scholar 

  • Jia W. J., Abe T., Futsuhara Y. 1987. Plant regeneration by pollen culture of rice. Rice Genet. Newsl. 4: 109–110.

    Google Scholar 

  • Jia X., Zhuang J., Hu S., Ye C., Nie D. 1994. Establishment and application of the medium of anther culture of intergeneric hybrids of Triticum aestivum × Triticum agropyron. Sci. Agri. Sin. 27: 83–87.

    Google Scholar 

  • Karsai I., Bedö Z., Hayes P. M. 1994. Effect of iduction medium pH and maltose consentration on in vitro androgenesis of hexaploid winter triticale and wheat. Plant Cell Tiss. Org. Cult. 39:49–53.

    Article  CAS  Google Scholar 

  • Kasha K. J., Maluszynski M. 2003. Production of doubled haploids in crop plants. An introduction. M. Maluszynski et al. (eds.): Doubled Haploid Production in Crop Plants. Kluwer Ac. Pub., pp.1–4.

  • Keller J. 1991. Influence of media conditioning on in vitro development in pollen suspensions of triticale. Biol. Zentrb. 110: 207–214.

    Google Scholar 

  • Lentini, Z., Reyes P., Martinez C. P., Roca W. M. 1995. Androgenesis in highly recalcitrant rice genotypes with maltose and silver nitrate. Plant Sci. 110: 127–138.

    Article  CAS  Google Scholar 

  • Liu W., Zheng M. Y., Konzak C. F. 2002. Improving green plant production via isolated microspore culture in bread wheat (Triticum aestivum L). Plant Cell Rep. 20: 821–824.

    Article  CAS  Google Scholar 

  • Marciniak K., Banaszsak Z., Wedzony M. 1998. Effect of genotype, medium and sugar on triticale (× Triticosecale Wittmack) anther culture. Cer. Res. Comm. 26: 145–151.

    Google Scholar 

  • Mejza S. J., Morgant V., Di Bona D. E., Wong J. R. 1993. Plant regeneration from isolated microspores of Triticum aestivum. Plant Cell Rep. 12: 149–153.

    Article  Google Scholar 

  • Monostori T., Lantos Cs., Mihály R., Pauk J. 2003. Induction of embryogenesis without exogenus hormone-supplement in barley microspore culture. Cer. Res. Comm. 31: 297–300.

    Google Scholar 

  • Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15: 473–497.

    Article  CAS  Google Scholar 

  • Ogava T., Fukuoka H., Okhawa Y. 1994. Induction of cell division of isolated pollen grains by sugar starvation in rice. Breed. Sci. 44: 75–77.

    Google Scholar 

  • Oleszczuk S., Sowa S., Zimny J. 2004. Direct embryogenesis and green plant regeneration from isolated microspores of hexaploid triticale (× Triticosecale Wittmack) cv. Bogo. Plant Cell Rep. 22: 885–893.

    CAS  Google Scholar 

  • Ouyang T. W., Hu J., Chuang C. C., Tseng C. C. 1973. Induction of pollen plants from anthers of Triticum aestivum L. cultured in vitro. Sci. Sin. 16: 79–95.

    Google Scholar 

  • Ouyang J. W., Zhou S. M., Jia S. E. 1983. The response of anther culture to culture temperature in Triticum aestivum. Theor. Appl. Genet. 66: 101–109.

    Article  Google Scholar 

  • Pauk J., Manninen O., Mattila I., Salo Y., Puli S. 1991. Androgenesis in hexaploid spring wheat F2 population and their parents using a multiple step regeneration system. Plant Breeding 107: 18–27.

    Article  Google Scholar 

  • Pauk J., Poulimatka M., Lökös Tóth K., Monostori T. 2000. In vitro androgenesis of triticale in isolated microspore culture. Plant Cell Tiss. Org. Cult. 61: 221–229.

    Article  CAS  Google Scholar 

  • Puolimatka M., Laine S., Pauk J. 1996. Effect of ovary co-cultivation and culture medium on embryogenesis of directly isolated microspores of wheat. Cer. Res. Comm. 23: 393–400.

    Google Scholar 

  • Purnhauser L., Gyulai G. 1993. Effect of copper on shoot and root regeneration in wheat, triticale, rape and tobacco tissue culture. Plant Cell Tiss. Org. Cult. 35: 131–139.

    Article  CAS  Google Scholar 

  • Raghavan V. 1988. Anther and pollen development in rice (Oryza sativa). Amer. J. Bot. 75: 183–196.

    Article  Google Scholar 

  • Raina S. K., Irfan S. T. 1998. High frequency embryogenesis and plantlet regeneration from isolated microspores of indica rice. Plant Cell Rep. 17: 957–962.

    Article  CAS  Google Scholar 

  • Simon-Kiss I. 2001. Six Decades of Rice Cultivation and Varietal Improvement in Hungary, Hungarian Agricult. Res. 10: 4–7.

    Google Scholar 

  • Sun C. S., Wang C. C., Chu C. C. 1973. Cytological studies on the androgenesis of Triticale. Acta Bot. Sin. 15: 145–154.

    Google Scholar 

  • Tuvesson I. K. D., Öhlund R. C. V. 1993. Plant regeneration through culture of isolated microspores of Triticum aestivum L. Plant Cell Tiss. Org. Cult. 34: 163–167.

    Article  Google Scholar 

  • Touraev A., Indrianto A., Wratschko I., Vicente O., Heberle Bors E. 1996. Efficient microspore embryogenesis in wheat (Triticum aestivum L.) induced by starvation at high temperatures. Sex. Plant Reprod. 9: 209–215.

    Article  Google Scholar 

  • Wang Y. Y., Sun C. S., Wang C. C., Chen N. F. 1973. The induction of pollen plantlets of Triticale and Capsicum annum from anther culture. Scientia Sin. 16: 147–151.

    Google Scholar 

  • Xie J., Gao M., Cai Q., Cheng X., Shen Y., Liang Z. 1995. Improved isolated microspore culture efficiency in medium with maltose and optimized growth regulator combination in japonica rice (Oryza sativa). Plant Cell Tiss. Org. Cult. 42: 245–250.

    Article  CAS  Google Scholar 

  • Zhuang J. J., Xu J. 1983. Increasing differentiation frequencies in wheat pollen callus. In: Cell and tissue culture techniques for cereal crop improvement, ed. by Hu H, Vega M.R., Science, Beijing: 431–431.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Wheat Genetics and Breeding, Cereal Research Non-profit Company, P. O. B. 391, H-6701, Szeged, Hungary

    Csaba Lantos, Mihály Jancsó & János Pauk

  2. Department of Genetics and Plant Breeding, St. Stephen University, Páter K. u. l., H-2103, Gödöll, Hungary

    Csaba Lantos & Mihály Jancsó

  3. Research Institute for Fisheries, Aquaculture and Irrigation, Anna liget 8, H-5540, Szarvas, Hungary

    Mihály Jancsó

Authors
  1. Csaba Lantos
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mihály Jancsó
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. János Pauk
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to János Pauk.

Additional information

HAS-SIU Research Group for Molecular Plant Breeding, Gödöll

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lantos, C., Jancsó, M. & Pauk, J. Microspore culture of small grain cereals. Acta Physiol Plant 27, 631–639 (2005). https://doi.org/10.1007/s11738-005-0067-6

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-005-0067-6

Key words

Search

Navigation