Abstract
Nodal segments obtained from in vitro proliferated shoots of Eclipta alba (L.) Hassk, were encapsulated in calcium alginate beads for large-scale clonal propagation, short-term conservation and germplasm exchange and distribution. The best gel complexation was achieved using 3% sodium alginate and 100 mM CaCl2·2H2O. Maximum percent response (100%) for conversion of encapsulated nodal segments into plantlets was obtained on 0.7% agar-solidified full-strength MS medium containing 0.88 μM BAP. Encapsulated nodal segments could be stored at low temperature (4°C) up to 60 days with a survival frequency of 51.2%. The well-developed plantlets regenerated from encapsulated nodal segments were hardened-off successfully with 90% survival frequency.
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Financial assistance provided by Council of Scientific & Industrial Research (CSIR), New Delhi, to the authors (MKR and PA) are gratefully acknowledged.
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Communicated by P. Wojtaszek.
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Singh, S.K., Rai, M.K., Asthana, P. et al. Alginate-encapsulation of nodal segments for propagation, short-term conservation and germplasm exchange and distribution of Eclipta alba (L.). Acta Physiol Plant 32, 607–610 (2010). https://doi.org/10.1007/s11738-009-0444-7
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DOI: https://doi.org/10.1007/s11738-009-0444-7