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Shoot regeneration from cotyledonary leaf explants of Jatropha curcas: a biodiesel plant

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Abstract

A simple, high frequency, and reproducible method for plant regeneration through direct organogenesis from cotyledonary leaf explants of Jatropha curcas was developed using Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) or 6-benzyl aminopurine (BAP). Medium containing TDZ has greater influence on regeneration as compared to BAP. The induced shoot buds were transferred to MS medium containing 10 μM kinetin (Kn), 4.5 μM BAP, and 5.5 μM α-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with different concentrations and combinations of BAP, indole-3-acetic acid (IAA), NAA, and indole-3-butyric acid (IBA). MS medium with 2.25 μM BAP and 8.5 μM IAA was found to be the best combination for shoot elongation. However, significant differences in plant regeneration and shoot elongation were observed among the genotypes studied. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing different concentrations and combinations of IBA, IAA, and NAA for 4 days, followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg l−1 activated charcoal. Elongated shoot treated with 15 μM IBA, 5.7 μM IAA, and 11 μM NAA resulted in highest percent rooting. The rooted plants could be established in soil with more than 90% survival rate. The method developed may be useful in improvement of J. curcas through genetic modification.

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References

  • Azad MAK, Yokota S, Ohkubo T, Andoh Y, Yahara S, Yoshizawa N (2005) In vitro regeneration of the medicinal woody plant Phellodendron amurense Rupr. through excised leaves. Plant Cell Tiss Organ Cult 80:43–50

    Article  Google Scholar 

  • Blakesey D (1991) Uptake and metabolosm of 6-benzyladenine in shoot proliferation of Musa and Rhododendron. Plant Cell Tiss Organ Cult 25:69–74

    Article  Google Scholar 

  • Chitra DS, Padmaja G (2005) Shoot regeneration via direct organogenesis from in vitro derived leaves of mulberry using thidiazuron and 6-benzylaminopurine. Sci Hortic 106:593–602

    Article  CAS  Google Scholar 

  • Christopher T, Rajam MV (1996) Effect of genotype, explant and medium on in vitro regeneration of red pepper. Plant Cell Tiss Organ Cult 46:245–250

    Article  CAS  Google Scholar 

  • da Camara Machado A, Frick NS, Kremen R, Katinger H, da Camara Machado ML (1997) Biotechnological approaches to the improvement of Jatropha curcas. In: Proceedings of the international symposium on jatropha, Nicaragua

  • Deore AC, Johnson TS (2008) High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L.: an important biodiesel plant. Plant Biotech Rep 2:10–15

    Google Scholar 

  • Feyissa T, Welander M, Negash L (2005) In vitro regeneration of Hagenia abyssinica (Bruce) J.F. Gmel. (Rosaceae) from leaf explants. Plant Cell Rep 24:392–400

    Article  CAS  PubMed  Google Scholar 

  • Francis G, Edingger R, Becker K (2005) A concept for simultaneous wasteland reclamation, fuel production, and socio-economic development in degraded areas in India. Need, potential and perspectives of Jatropha plantations. Nat Res Forum 29:12–24

    Article  Google Scholar 

  • Ghosh A, Chaudhary DR, Reddy MP, Rao SN, Chikara J, Pandya JB, Patolia JS, Gandhi MR, Adimurthy S, Vaghela N, Mishra S, Rathod MR, Prakash AR, Shethia BD, Upadhyay SC, Balakrishna V, Prakash CR, Ghosh PK (2007) Prospects for Jatropha methyl ester (biodiesel) in India. Int J Environ Stud 64:659–674

    Article  CAS  Google Scholar 

  • Guda CD, Castello S, Savona M, Farina E (2003) Echinacea purpurea—in vivo and in vitro propagation and field evaluation of ornamental clones. Col Prot 32:101–107

    Google Scholar 

  • Hare PD, Van Staden J (1994) Inhibitory effect of thidiazuron on the activity of cytokinin oxidase isolated from soybean callus. Plant Cell Physiol 35:1121–1125

    CAS  Google Scholar 

  • Henry Y, Vain P, Buyser JD (1994) Genetic analysis of in vitro plant tissue culture responses and regeneration capacities. Euphytica 79:45–58

    Article  Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Organ Cul 33:105–119

    Article  CAS  Google Scholar 

  • Hyde CL, Phillips GC (1996) Silver nitrate promotes shoot development and plant regeneration of Chilepepper (Capsicum annuum L.) via organogenesis. In Vitro Cell Dev Biol Plant 32:72–80

    CAS  Google Scholar 

  • Jha T, Mukherjee P, Datta MM (2007) Somatic embryogenesis in Jatropha curcas Linn., an important biofuel plant. Plant Biotech Rep 1:135–140

    Article  Google Scholar 

  • Kaminek M (1992) Progress in cytokinin research. Trends Biotech 10:159–162

    Article  CAS  Google Scholar 

  • Koroch A, Juliani HR, Kapteyn J, Simon JE (2002) In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tiss Organ Cult 69:79–83

    Article  CAS  Google Scholar 

  • Kumar N, Pamidimarri SDVN, Kaur M, Boricha G, Reddy MP (2008) Effects of NaCl on growth, ion accumulation, protein, proline contents, and antioxidant enzymes activity in callus cultures of Jatropha curcas. Biologia 63:378–382

    Article  CAS  Google Scholar 

  • Lemaux PG (2008) Genetically engineered plants and foods: a scientist’s analysis of the issues (Part I). Annu Rev Plant Biol 59:771–812

    Article  CAS  PubMed  Google Scholar 

  • Lin HS, De Jeu MJ, Jacobsen E (1997) Direct shoot regeneration from excised leaf explants of in vitro grown seedlings of Alstroemeria L. Plant Cell Rep 16:770–774

    Article  CAS  Google Scholar 

  • Magioli C, Rocha APM, de Oliveira DE, Mansur E (1998) Efficient shoot organogenesis of eggplant (Solanum melongena L.) induced by thidiazuron. Plant Cell Rep 17:661–663

    Article  CAS  Google Scholar 

  • Martin KP (2003) Rapid axillary bud proliferation and ex vitro rooting of Eupatorium triplinerve. Biol Plant 47:589–591

    Article  CAS  Google Scholar 

  • Mondal TK, Bhattacharya A, Sood A, Ahuja PS (1998) Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) using thidiazuron. Plant Growth Regul 26:57–61

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479

    Article  CAS  Google Scholar 

  • Nandagopal S, Ranjitha Kumari BD (2007) Effectiveness of auxin induced in vitro root culture in chicory. J Cen Eur Agri 8:73–79

    Google Scholar 

  • Nielsen JM, Kirsten B, Hansen J (1993) Long-term effects of thidiazuron are intermediate between benzyladenine, kinetin or isopentenyladenine in Miscanthus sinensis. Plant Cell Tiss Organ Cult 35:173–179

    Article  CAS  Google Scholar 

  • Openshaw K (2000) A reviews of Jatropha curcas: an oil plant of unfulfilled promise. Biomass Bioeng 19:1–5

    Article  Google Scholar 

  • Ozaslan M, Can C, Aytekin T (2005) Effect of explant source on in vitro propagation of Paulownia tomentosa Steud. Biotech Biotech Equ 19:20–26

    CAS  Google Scholar 

  • Pellegrineschi A (1997) In vitro plant regeneration via organogenesis of cowpea [Vigna unguiculata (L.) Walp.]. Plant Cell Rep 17:89–95

    Article  CAS  Google Scholar 

  • Preece JE, Imel MR (1991) Plant regeneration from leaf explants of Rhododendron ‘P.J.M. Hybrids’. Sci Hortic 48:159–170

    Article  CAS  Google Scholar 

  • Raghu AV, Geetha SP, Martin G, Balachandran I, Ravindran PN (2006) Direct organogenesis from leaf explants of Embelia ribes Burm.—a vulnerable medicinal plant. J For Res 11:57–60

    Article  Google Scholar 

  • Rajore S, Batra A (2007) An alternative source for regenerable organogenic callus induction in Jatropha curcas. Ind J Biotech 6:545–548

    Google Scholar 

  • Reddy MP, Kumar N, Vijay Anand KG, Singh AH, Singh S (2008) Method for micropropagation of Jatropha curcas plants from leaf explants (Patent filed US and PCT, File No. 2537de2008).

  • Schween G, Schwenkel HG (2003) Effect of genotype on callus induction, shoot regeneration, and phenotypic stability of regenerated plants in greenhouse of Primula ssp. Plant Cell Tiss Organ Cult 72:53–61

    Article  CAS  Google Scholar 

  • Sharma KK, Bhatnagar-Mathur P, Thorpe TA (2005) Genetic transformation technology: status and problems. In Vitro Cell Dev Biol Plant 41:102–112

    Article  CAS  Google Scholar 

  • Singh A, Reddy MP, Patolia JS (2008) An improved protocol for micropropagation of elite genotypes of Simmondsia chinensis (Link) Schneider. Biol Plant 52:538–540

    Article  CAS  Google Scholar 

  • Sujatha M, Mukta N (1996) Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant Cell Tissue Organ Cult 44:135–141

    Article  Google Scholar 

  • Sujatha M, Makkar HPS, Becker K (2005) Shoot bud proliferation from axillary nodes and leaf sections of non-toxic Jatropha curcas L. Plant Growth Regul 47:83–90

    Article  CAS  Google Scholar 

  • Venkataiah P, Subhash K (2003) Thidiazuron-induced adventitious shoot bud formation and plant regeneration in Capsicum annuum L. J Plant Biotech 5:245–250

    Google Scholar 

  • Vuylasteker C, Dewaele S, Rambour S (1998) Auxin induced lateral root formation in chicory. Ann Bot 81:449–454

    Article  Google Scholar 

  • Wei Q, Wei-Da L, Liao Y, Shu-Lin P, Xu Y, Tang L, Fang C (2004) Plant regeneration from epicotyl explants of Jatropha curcas. J Plant Physiol Mol Biol 30:475–478

    Google Scholar 

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Acknowledgments

The authors gratefully acknowledge Council of Scientific and Industrial Research, New Delhi, India for financial assistance. Statistical analysis support from Mr. Keyur Shah and Mr. Amritpal singh, Assistant professor, Department of Agricultural Biotechnology, Anand Agricultural University, Anand, India is thankfully acknowledged.

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Author notes

  1. Nitish Kumar & Muppala P. Reddy

    Present address: Department of Agricultural Biotechnology, Anand Agricultural University, Anand, 388110, Gujarat, India

Authors and Affiliations

  1. Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute (Council of Scientific and Industrial Research, New Delhi), Bhavnagar, 364021, Gujarat, India

    Nitish Kumar, K. G. Vijay Anand & Muppala P. Reddy

  2. Plant Stress Genomics and Technology Center, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Kingdom of Saudi Arabia

    Muppala P. Reddy

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  1. Nitish Kumar
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  2. K. G. Vijay Anand
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  3. Muppala P. Reddy
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Correspondence to Muppala P. Reddy.

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Communicated by E. Lojkowska.

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Kumar, N., Vijay Anand, K.G. & Reddy, M.P. Shoot regeneration from cotyledonary leaf explants of Jatropha curcas: a biodiesel plant. Acta Physiol Plant 32, 917–924 (2010). https://doi.org/10.1007/s11738-010-0479-9

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  • DOI: https://doi.org/10.1007/s11738-010-0479-9

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