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In vitro propagation and assessment of clonal fidelity of Nepenthes khasiana Hook. f.: a medicinal insectivorous plant of India

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Abstract

An efficient in vitro protocol for large-scale multiplication of Nepenthes khasiana, a threatened insectivorous plant of India, has been developed from nodal stem segments. The highest shoot proliferation of 19.16 ± 0.23 shoots/explant was recorded in half-strength Murashige and Skoog (MS) medium supplemented with 2.5 mg/l kinetin, 2.0 mg/l 6-benzyl aminopurine, 3 % sucrose and 0.8 % agar. The best rooting was achieved in half-strength MS medium supplemented with 2.0 mg/l α-naphthalene acetic acid with an average of 9.04 ± 0.46 roots/shoot. The plantlets were successfully transferred to the greenhouse with survival rate of 92 %, exhibiting normal development. Cytological and random amplified polymorphic DNA (RAPD) analyses were carried out to assess the genetic integrity of the regenerated plantlets. Cytological analysis revealed no change in chromosome number with cells studied showing 2n = 80. Of the 80 primers screened for RAPD analysis, 14 primers resulted in clear and scorable bands. A total of 72 amplification products were obtained out of which only 4.1 % bands were polymorphic. Cluster analysis of the RAPD profile revealed an average similarity coefficient ranging from 0.98 to 1.0, thus suggesting genetic stability in the micropropagated plants of N. khasiana.

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Acknowledgments

The study was supported by Research Grant No. BT/04/NE/2009 from Department of Biotechnology, Government of India, New Delhi, India.

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Authors and Affiliations

  1. Department of Botany, North-Eastern Hill University, Shillong, 793022, India

    Soibam Purnima Devi, Suman Kumaria & Pramod Tandon

  2. Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, 793022, India

    Satyawada Rama Rao

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  1. Soibam Purnima Devi
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  2. Suman Kumaria
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  3. Satyawada Rama Rao
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  4. Pramod Tandon
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Correspondence to Suman Kumaria.

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Communicated by E. Lojkowska.

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Devi, S.P., Kumaria, S., Rao, S.R. et al. In vitro propagation and assessment of clonal fidelity of Nepenthes khasiana Hook. f.: a medicinal insectivorous plant of India. Acta Physiol Plant 35, 2813–2820 (2013). https://doi.org/10.1007/s11738-013-1314-x

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