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Changes in expression of proteins involved in alleviation of Fe-deficiency by sulfur nutrition in Brassica napus L.

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Abstract

In order to characterize the significance of sulfur (S) nutrition in protein expression under iron (Fe)-deficient conditions, gel-based proteomic analysis was performed with the leaves of Brassica napus exposed to S and Fe combined treatments: sufficient in S and Fe (+S/+Fe, control), sufficient S but Fe deprived (+S/−Fe), deprived S but sufficient Fe (−S/+Fe), and deprived S and Fe (−S/−Fe). The resulting data showed that 15 proteins were down-regulated due to production of oxidative damage as indicated by H2O2 and O −12 localizations and due to leaf chlorosis in leaves in S-deprived leaves either in presence (−S/+Fe) or absence of Fe (−S/−Fe), whereas these down-regulated proteins were well expressed in the presence of S (+S/−Fe) compared to control (+S/+Fe). In addition, two proteins were up-regulated under S-deprived condition in presence (−S/+Fe) and absence of (−S/−Fe) Fe. The functional classification of these identified proteins was estimated that 40 % of the proteins belong to chloroplast precursor, and rest of the proteins belongs to hypothetical proteins, RNA binding, secondary metabolism and unknown proteins. On the other hand, five protein spots from S deprived (−S/+Fe) and ten spots from Fe deprived (−S/−Fe) conditions were absent, whereas they were well expressed in presence of S (+S/−Fe) compared to control plants (+S/+Fe). These results suggest that sulfur nutrition plays an important role in alleviating protein damage in Fe-deficient plants and adaptation to Fe-deficiency in oilseed rape.

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Abbreviations

2DE:

Two-dimensional gel electrophoresis

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

MALDI-TOF:

Matrix-assisted laser desorption/ionization-time of flight

IPG:

Immobilized pH gradient

IAA:

Iodoacetamide

DTT:

Dithiothreitol

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Acknowledgments

This study was financially supported by Chonnam National University, 2012.

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Authors and Affiliations

  1. Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University, Buk-Gwangju, P.O. Box 205, Gwangju, 500-600, Korea

    Sowbiya Muneer & Tae Hwan Kim

  2. Environmental Friendly Agricultural Bio-material Research Team, College of Agriculture, Chonnam National University, Gwangju, 500-757, Korea

    Bok Rye Lee

  3. Central Instrument Facility, Biomaterial Analytical Lab, Gyeongsang National University, Jinju, 660-701, Korea

    Dong Won Bae

Authors
  1. Sowbiya Muneer
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  2. Bok Rye Lee
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  3. Dong Won Bae
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  4. Tae Hwan Kim
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Correspondence to Tae Hwan Kim.

Additional information

Communicated by J.-H. Liu.

T. H. Kim: Deceased.

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Muneer, S., Lee, B.R., Bae, D.W. et al. Changes in expression of proteins involved in alleviation of Fe-deficiency by sulfur nutrition in Brassica napus L.. Acta Physiol Plant 35, 3037–3045 (2013). https://doi.org/10.1007/s11738-013-1336-4

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  • DOI: https://doi.org/10.1007/s11738-013-1336-4

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