Skip to main content
SpringerLink
Log in

Effect of phosphorus starvation on hormone content and growth of barley plants

  • Original Article
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

To address the question of the role of phytohormones in the growth responses induced by P availability, we compared the effects of low P on the growth of barley plants, and the contents of auxin, cytokinins and abscisic acid (ABA). Comparative study of the changes in growth and hormones’ levels in response to P-starvation showed that relative activation of root growth may be related to the decline in shoot cytokinin content and ABA accumulation in the roots of P-starved (P−) barley plants. The decline in shoot cytokinins is likely to result from the inhibition of the transport of these hormones from roots, and in turn, may contribute to increased distribution of auxins in favor of roots. Reduced root branching detected in our experiments, despite maintenance of root auxins, may be related to an elevated level of either cytokinins or ABA in roots of P− barley plants. Thus, interactions between auxins, cytokinins and ABA are likely to be responsible for the changes in root architecture in P− plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Aibara I, Miwa K (2014) Strategies for optimization of mineral nutrient transport in plants: multilevel regulation of nutrient-dependent dynamics of root architecture and transporter activity. Plant Cell Physiol 55:2027–2036

    Article  PubMed  Google Scholar 

  • Arkhipova TN, Veselov SU, Melentiev AI, Martynenko EV, Kudoyarova GR (2005) Ability of bacterium Bacillus subtilis to produce cytokinins and to influence the growth and endogenous hormone content of lettuce plants. Plant Soil 27:201–209

    Article  Google Scholar 

  • Arkhipova TN, Prinsen E, Veselov SU, Martineko EV, Melentiev AI, Kudoyarova GR (2007) Cytokinin producing bacteria enhances plant growth in drying soil. Plant Soil 292:305–315

    Article  CAS  Google Scholar 

  • Casimiro I, Beeckman T, Graham N, Bhalerao R, Zhang H, Casero P, Sandberg G, Bennett MJ (2003) Dissecting Arabidopsis lateral root development. Trends Plant Sci 8:165–171

    Article  CAS  PubMed  Google Scholar 

  • Drew MC (1975) Comparison of the effects of a localized supply of phosphate, nitrate, ammonium and potassium on the growth of the seminal root system, and the shoot of barley. New Phytol 75:479–490

    Article  CAS  Google Scholar 

  • Hachiya T, Sugiura D, Kojima M, Sato S, Yanagisawa S, Sakakibara H, Terashima I, Noguchi K (2014) High CO2 triggers preferential root growth of Arabidopsis thaliana via two distinct systems under low pH and low N stresses. Plant Cell Physiol 55:269–280

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hammond JP, White PJ (2008) Sucrose transport in the phloem: integrating root responses to phosphorus starvation. J Exp Bot 59:93–109

    Article  CAS  PubMed  Google Scholar 

  • Hermans C, Hammond JP, White PJ, Verbruggen N (2006) How do plants respond to nutrient shortage by biomass allocation? Trends Plant Sci 11:610–617

    Article  CAS  PubMed  Google Scholar 

  • Kudoyarova GR, Korobova AV, Akhiyarova GR, Arkhipova TN, Zaytsev DY, Prinsen E, Egutkin NL, Medvedev SS, Veselov SY (2014) Accumulation of cytokinins in roots and their export to the shoots of durum wheat plants treated with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP). J Exp Bot 65:2287–2294

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kudoyarova GR, Dodd IC, Veselov DS, Rothwell SA, Veselov SY (2015) Common and specific responses to availability of mineral nutrients and water. J Exp Bot 66:2133–2144

    Article  CAS  PubMed  Google Scholar 

  • Lopez-Bucio J, Hernández-Abreu E, Sánchez-Calderón L, Nieto-Jacobo MF, Simpson J, Herrera-Estrella L (2002) Phosphate availability alters architecture and causes changes in hormone sensitivity in the Arabidopsis root system. Plant Physiol 129:244–256

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lynch JP (2011) Root phenes for enhanced soil exploration and phosphorus acquisition: tools for future crops. Plant Physiol 156:1041–1049

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Martin AC, Del Pozo JC, Iglesias J, Rubio V, Solano R, de la Pena A, Leyva A, Paz-Ares J (2000) Influence of cytokinins on the expression of phosphate starvation responsive genes in Arabidopsis. Plant J 24:559–568

    Article  CAS  PubMed  Google Scholar 

  • Moubayidin L, Di Mambro R, Sabatini S (2009) Cytokinin-auxin crosstalk. Trends Plant Sci 14:557–562

    Article  CAS  PubMed  Google Scholar 

  • Niu YF, Chai RS, Jin GL, Wang H, Tang CX, Zhang YS (2013) Responses of root architecture development to low phosphorous availability: a review. Ann Bot 112:391–408

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Park MR, So-Hyeon Baek S-H, Reyes BG, Yun SJ, Hasenstein KH (2012) Transcriptome profiling characterizes phosphate deficiency effects on carbohydrate metabolism in rice leaves. J Plant Physiol 169:193–205

    Article  CAS  PubMed  Google Scholar 

  • Perez-Torres CA, Lopez-Bucio J, Cruz-Ramırez A, Ibarra-Laclette E, Dharmasiri S, Estelle M, Herrera-Estrella L (2008) Phosphate availability alters lateral root development in Arabidopsis by modulating auxin sensitivity via a mechanism involving the TIR1 auxin receptor. Plant Cell 20:3258–3272

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Puig J, Pauluzzi G, Guiderdoni E, Gantet P (2012) Regulation of shoot and root development through mutual signalling. Mol Plant 5:974–983

    Article  CAS  PubMed  Google Scholar 

  • Ribot C, Wang Y, Poirier Y (2008) Expression analyses of three members of the AtPHO1 family reveal differential interactions between signaling pathways involved in phosphate deficiency and the responses to auxin, cytokinin, and abscisic acid. Planta 227:1025–1036

    Article  CAS  PubMed  Google Scholar 

  • Rubio V, Bustos R, Irigoyen ML, Cardona-Lopez X, Rojas-Triana M, Paz-Ares J (2009) Plant hormones and nutrient signaling. Plant Mol Biol 69:361–373

    Article  CAS  PubMed  Google Scholar 

  • Saab IN, Sharp RE, Pritchard J, Voetberg GS (1990) Increased endogenous abscisic acid maintains primary root growth and inhibits shoot growth of maize seedlings at low water potentials. Plant Physiol 93:1329–1336

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sanchez-Calderon L, Lopez-Bucio J, Chacon-Lopez A, Cruz-Ramırez A, Nieto-Jacobo F, Dubrovsky JG, Herrera-Estrella L (2005) Phosphate starvation induces a determinate developmental program in the roots of Arabidopsis thaliana. Plant Cell Physiol 46:174–184

    Article  CAS  PubMed  Google Scholar 

  • Shen C, Yue R, Yang Y, Zhang L, Sun T et al (2014) OsARF16 is involved in cytokinin-mediated inhibition of phosphate transport and phosphate signaling in rice (Oryza sativa L.). PLoS One 9(11):e112906. doi:10.1371/journal.pone.0112906

    Article  PubMed  PubMed Central  Google Scholar 

  • Shkolnik-Inbar D, Bar-Zvi D (2010) ABI4 mediates abscisic acid and cytokinin inhibition of lateral root formation by reducing polar auxin transport in Arabidopsis. Plant Cell 22:3560–3573

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Talboys PJ, Healey JR, Withers PJA, Jones DL (2014) Phosphate depletion modulates auxin transport in Triticum aestivum leading to altered root branching. J Exp Bot 65:5023–5032

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Taussky HH, Shorr E (1953) A microcolorimetric method for the determination of inorganic phosphorus. J Biol Chem 202:675–685

    CAS  PubMed  Google Scholar 

  • Veselov SU, Kudoyarova GR, Egutkin NL, Gyuli-Zade VG, Mustafina AR, Kof EK (1992) Modified solvent partitioning scheme providing increased specificity and rapidity of immunoassay for indole 3-acetic acid. Physiol Plantar 86:93–96

    Article  CAS  Google Scholar 

  • Veselov SU, Valcke R, Van Onckelen H, Kudoyarova G (1999) Cytokinin content and location in the leaves of the wild-type and transgenic tobacco plants. Russ J Plant Physiol 46:26–33

    CAS  Google Scholar 

  • Vysotskaya LB, Korobova AV, Kudoyarova GR (2008) Abscisic acid accumulation in the roots of nutrient-limited plants: its impact on the differential growth of roots and shoots. J Plant Physiol 165:1274–1279

    Article  CAS  PubMed  Google Scholar 

  • Vysotskaya LB, Korobova AV, Veselov SY, Dodd IC, Kudoyarova GR (2009) ABA mediation of shoot cytokinin oxidase activity: assessing its impacts on cytokinin status and biomass allocation of nutrient deprived durum wheat. Func Plant Biol 36:66–72

    Article  CAS  Google Scholar 

  • Wang SK, Zhang AN, Sun CD, Xu YX, Chen Y, Yu CL, Qian Q, Jiang D-A, Qi YH (2014) Auxin response factor (OsARF12), a novel regulator for phosphate homeostasis in rice (Oryza sativa). New Phytol 201:91–103

    Article  CAS  PubMed  Google Scholar 

  • Wang Y-L, Almvik M, Clarke N, Eich-Greatorex S, Ogaard AF, Krogstad T, Lambers H, Clarke JL (2015) Contrasting responses of root morphology and root-exuded organic acids to low phosphorus availability in three important food crops with divergent root traits. AoB Plants 7:plv097

    Article  PubMed  PubMed Central  Google Scholar 

  • Wilkinson S, Kudoyarova GR, Veselov DS, Arkhipova TN, Davies WJ (2012) Plant hormone interactions: innovative targets for crop breeding and management. J Exp Bot 63:3499–3509

    Article  CAS  PubMed  Google Scholar 

  • Zhang C, Bousquet A, Harris JM (2014) Abscisic acid and lateral root organ defective/numerous infections and polyphenolics modulate root elongation via reactive oxygen species in Medicago truncatula. Plant Physiol 166:644–658

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The work was partially supported by the Russian Foundation for Basic Research Grant No. 15-04-04750.

Author information

Authors and Affiliations

  1. Ufa Institute of Biology, Russian Academy of Sciences, pr. Oktyabrya 69, 450054, Ufa, Russian Federation

    Lidiya B. Vysotskaya, Arina W. Trekozova & Guzel R. Kudoyarova

Authors
  1. Lidiya B. Vysotskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Arina W. Trekozova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Guzel R. Kudoyarova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guzel R. Kudoyarova.

Additional information

Communicated by NA Anjum.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vysotskaya, L.B., Trekozova, A.W. & Kudoyarova, G.R. Effect of phosphorus starvation on hormone content and growth of barley plants. Acta Physiol Plant 38, 108 (2016). https://doi.org/10.1007/s11738-016-2127-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11738-016-2127-5

Keywords

Search

Navigation