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Phenotypic characterization of a rice mutant Oryza sativa extraordinary glume 1 (Oseg 1) and its genetic analysis

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Journal of Shanghai University (English Edition)

Abstract

A rice mutant with Japonica 9522 cultivar background Oryza sativa extraordinary glume 1 (Oseg 1) was identified from the M 2 mutant pool mutagenized by 60Co γ-ray. Compared with wild type plants, Oseg 1 developed longer empty glumes and rudimentary glumes. In some Oseg 1 mutants, the number of stamens of flowers was reduced and leaf-like lodicules occurred, and excessive lemma/palea-like organ could be observed in some mutant spikelets. This indicated that OsEG1 could regulate the edevelopment of rudimentary glumes, empty glumes, lemma/palea, lodicules, and stamens. Genetic analysis indicated that Oseg 1 came from a single recessive genetic locus. To clone OsEG1 gene, F 2 population was constructed by a cross between Oseg 1 (Japonica) and Guangluai4 (Indica). Using map-based cloning approach, OsEG1 was mapped on chromosome 4, between INDEL marker OS407 and WHM0466 with genetic distance of 2.0 cm and 1.0 cm, respectively. These results are useful for further cloning and functional analysis of the OsEG1 gene.

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Authors and Affiliations

  1. School of Life Sciences, Shanghai University, Shanghai, 200444, P. R. China

    Wang Hong-mei  (王红梅) & Yong Ke-lan  (雍克岚)

  2. SJTU-SIBS-PSU Joint Center for Life Sciences, School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200240, P. R. China

    Wang Hong-mei  (王红梅), Chu Huang-wei  (储黄伟), Liu Hai-sheng  (刘海生), Li Xiao-xing  (李晓星), Yang Gong-da  (杨功达) & Zhang Da-bing  (张大兵)

Authors
  1. Wang Hong-mei  (王红梅)
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  2. Chu Huang-wei  (储黄伟)
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  3. Liu Hai-sheng  (刘海生)
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  4. Li Xiao-xing  (李晓星)
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  5. Yang Gong-da  (杨功达)
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  6. Zhang Da-bing  (张大兵)
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  7. Yong Ke-lan  (雍克岚)
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Correspondence to Yong Ke-lan  (雍克岚).

Additional information

Project supported by the National Key Basic Research Development Program of the Ministry of Science and Technology of China (Grant Nos.2001CB109002, 2005CB120802), the National High-Technology Research and Development Program of China (Grant No.2005AA2710330), the Science Foundation of Shanghai Municipal Commission of Science and Technology (Grant Nos.03JC14061, 03DJ14016), the Program for New Century Excellent Talents in University (Grant No.NCET-04-0403), and the Shuguang Plan of Shanghai Education Development Foundation (Grant No.04SG15)

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Wang, Hm., Chu, Hw., Liu, Hs. et al. Phenotypic characterization of a rice mutant Oryza sativa extraordinary glume 1 (Oseg 1) and its genetic analysis. J. Shanghai Univ. 11, 619–624 (2007). https://doi.org/10.1007/s11741-007-0618-1

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  • DOI: https://doi.org/10.1007/s11741-007-0618-1

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