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Co-production of lipids, eicosapentaenoic acid, fucoxanthin, and chrysolaminarin by Phaeodactylum tricornutum cultured in a flat-plate photobioreactor under varying nitrogen conditions

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Abstract

The marine diatom Phaeodactylum tricornutum is a polymorphological, ecologically significant, and well-studied model of unicellular microalga. This diatom can accumulate diverse important metabolites. Herein, we cultured P. tricornutum in an internally installed tie-piece flat-plate photobioreactor under 14.5 m mol L−1 (high nitrogen, HN) and 2.9 m mol L−1 (low nitrogen, LN) of KNO3 and assessed its time-resolved changes in biochemical compositions. The results showed that HN was inductive to accumulate high biomass (4.1 g L−1). However, the LN condition could accelerate lipid accumulation in P. tricornutum. The maximum total lipid (TL) content under LN was up to 42.5% of biomass on day 12. Finally, neutral lipids (NLs) were 63.8% and 75.7% of TLs under HN and LN, respectively. The content of EPA ranged from 2.3% to 1.5% of dry weight during the growth period under the two culture conditions. Peak volumetric lipid productivity of 128.4 mg L−1d−1 was achieved in the HN group (on day 9). The highest volumetric productivity values of EPA, chrysolaminarin, and fucoxanthin were obtained in the exponential phase (on day 6) under HN, which were 9.6, 93.6, and 4.7 mg L−1d−1, respectively. In conclusion, extractable amounts of lipids, EPA, fucoxanthin, and chrysolaminarin could be obtained from P. tricornutum by regulating the culture conditions.

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Acknowledgement

This work was supported by the following fundings: the Natural Science Foundation of China (No. 31170337); the National High Technology Research and Development Program of China (863 Program) (No. 2013AA 065805); the National Basic Research Program of China (973 Program) (No. 2011CB2009001); the Special Program for Low-Carbon, Reform and Development Commission of Guangdong Province (No. 2011-051).

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  1. Department of Ecology & Research Center for Hydrobiology, Jinan University, Guangzhou, 510632, P. R. China

    Baoyan Gao, Ailing Chen, Wenyuan Zhang, Aifen Li & Chengwu Zhang

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  1. Baoyan Gao
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  2. Ailing Chen
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  3. Wenyuan Zhang
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Correspondence to Chengwu Zhang.

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Gao, B., Chen, A., Zhang, W. et al. Co-production of lipids, eicosapentaenoic acid, fucoxanthin, and chrysolaminarin by Phaeodactylum tricornutum cultured in a flat-plate photobioreactor under varying nitrogen conditions. J. Ocean Univ. China 16, 916–924 (2017). https://doi.org/10.1007/s11802-017-3174-2

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