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Fractioned High Pressure Extraction of Anthocyanins from Elderberry (Sambucus nigra L.) Pomace

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Abstract

Fractionated high pressure extractions from dry and in natura elderberry pomace were performed in order to obtain anthocyanin rich extracts. Experiments were carried out using CO2 supercritical fluid extraction followed by enhanced solvent extraction (ESE) with CO2/EtOH–H2O mixtures (1–100%, v/v), to obtain anthocyanin rich fractions in the second step, at 313 K and ~20 MPa. Higher extract yields, anthocyanin contents and antioxidant activities occurred by the presence of water, both in the raw material and in the solvent mixture. The CO2 dissolved in the ESE solvent mixture favored either anthocyanin contents or antioxidant activities, which were not directly related. Comparing to the literature data for elderberries and grapes, these fractions had higher anthocyanins contents. From these results, an added economical value to this agroindustrial residue is proposed, using solvents and techniques “generally regarded as safe” in the food and pharmaceutical industries.

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Acknowledgments

The authors are grateful to Cooperativa Agrícola do Vale do Varosa (Portugal), for providing the raw material, and to Programa Iberoamericano de Ciencia y Tecnología para el Desarollo for technical support of the Aplicaciones Industriales de los Taninos Vegetales Project.

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Authors and Affiliations

  1. CIEPQPF, Department of Chemical Engineering, University of Coimbra, 3030-790, Coimbra, Portugal

    Inês J. Seabra, Mara E. M. Braga & Hermínio C. de Sousa

  2. ESAC, Politechnic Institute of Coimbra, Bencanta, 3040-316, Coimbra, Portugal

    Inês J. Seabra

  3. CEF, Pharmacognosy Laboratory, Faculty of Pharmacy, University of Coimbra, 3000-295, Coimbra, Portugal

    Maria T. P. Batista

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  1. Inês J. Seabra
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  2. Mara E. M. Braga
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  3. Maria T. P. Batista
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  4. Hermínio C. de Sousa
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Correspondence to Hermínio C. de Sousa.

Additional information

Grants support: I. J. Seabra and M. E. M. Braga acknowledge Fundação para a Ciência e Tecnologia (FCT-MCTES) for the doctoral and post-doctoral fellowships, respectively (SFRH/BD/29133/2006 and SFRH/BPD/21076/2004).

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Seabra, I.J., Braga, M.E.M., Batista, M.T.P. et al. Fractioned High Pressure Extraction of Anthocyanins from Elderberry (Sambucus nigra L.) Pomace. Food Bioprocess Technol 3, 674–683 (2010). https://doi.org/10.1007/s11947-008-0134-2

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