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Comparison Between Systems for Synthesis of Fructooligosaccharides from Sucrose Using Free Inulinase from Kluyveromyces marxianus NRRL Y-7571

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Abstract

This work is focused on the synthesis of the fructooligosaccharides (FOS) from sucrose using free inulinase from Kluyveromyces marxianus NRRL Y-7571 in aqueous and aqueous–organic systems. The most significant variables for the aqueous–organic system were identified using a fractional factorial design. The evaluated variables were the temperature, pH, sucrose concentration, inulinase activity, aqueous/organic ratio, and the polyethylene glycol concentration. The use of sequential experimental design methodology was shown to be very useful in the optimization of the FOS synthesis by inulinase either in aqueous or aqueous–organic systems. For the aqueous–organic system, the maximum Y FOS reached was 16.7 ± 1.1 wt.% with the following operational conditions: temperature of 40 °C, enzyme activity of 4 U mL−1, organic solvent/total system ratio of 25/100, pH of 6.0, and sucrose concentration of 55%. In the aqueous system, the maximum conversion obtained was 12.8 ± 1.0 wt.% under the following conditions: 40 °C, pH 5.0, 55% sucrose, and inulinase activity 4 U mL−1.

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Acknowledgments

The authors acknowledge the financial support of CAPES and FAPESP.

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Authors and Affiliations

  1. Department of Food Engineering, Faculty of Food Engineering, University of Campinas—UNICAMP, P.O. Box 6121, CEP 13083-862, Campinas, SP, Brazil

    Fernanda V. A. Risso, Marcio A. Mazutti, Fátima Costa, Francisco Maugeri & Maria Isabel Rodrigues

  2. Department of Food Engineering, URI—Campus de Erechim, P.O. Box 743, CEP 99700-000, Erechim, RS, Brazil

    Marcio A. Mazutti & Helen Treichel

Authors
  1. Fernanda V. A. Risso
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  2. Marcio A. Mazutti
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  3. Helen Treichel
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  4. Fátima Costa
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  5. Francisco Maugeri
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  6. Maria Isabel Rodrigues
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Correspondence to Marcio A. Mazutti or Helen Treichel.

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Risso, F.V.A., Mazutti, M.A., Treichel, H. et al. Comparison Between Systems for Synthesis of Fructooligosaccharides from Sucrose Using Free Inulinase from Kluyveromyces marxianus NRRL Y-7571. Food Bioprocess Technol 5, 331–337 (2012). https://doi.org/10.1007/s11947-009-0272-1

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