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Enzyme-Assisted Production of Tomato Seed Oil Enriched with Lycopene from Tomato Pomace

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Abstract

Large amounts of a waste known as tomato pomace and consisting mainly of the fruit peel and seeds are generated annually from the industrial processing of tomatoes. This material is rich in lycopene, a phytochemical with antioxidant and chemopreventive properties, and contains many valuable nutrients. In this study, we have investigated the possibility of using the whole waste to produce a lycopene-enriched seed oil. The oil was obtained by cold-pressing the seeds and was subsequently enriched in lycopene (up to 500 ppm) by incorporation of a tomato oleoresin derived from the peels. To increase lycopene recovery, the peels were pretreated with cell-wall-degrading enzymes and solvent extracted. This procedure allowed the production of about 25 kg/ton oleoresin with an average lycopene content of 6.8 wt.%. The compositional characteristics of the oil combined with the production of significant amounts of oleoresin strongly support the use of tomato pomace for producing lycopene-based functional products.

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References

  • Altan, A., McCarthy, K. L., & Maskana, M. (2008). Evaluation of snack foods from barley–tomato pomace blends by extrusion processing. Journal of Food Engineering, 84, 231–242.

    Article  Google Scholar 

  • Andreassi, L. (2011). UV exposure as a risk factor for skin cancer. Expert Review of Dermatology, 6, 445–454.

    Article  CAS  Google Scholar 

  • Anunciato, T. P., & da Rocha Filho, P. A. (2012). Carotenoids and polyphenols in nutricosmetics, nutraceuticals and cosmeceuticals. Journal of Cosmetic Dermatology, 11, 51–54.

    Article  Google Scholar 

  • Bovy, A., Schijlen, E., & Hall, R. D. (2007). Metabolic engineering of flavonoids in tomato (Solanum lycopersicum): the potential for metabolomics. Metabolomics, 3, 399–412.

    Article  CAS  Google Scholar 

  • Caris-Veyrat, C. C., Schmid, A., Carail, M., & Böhm, V. (2003). Cleavage products of lycopene produced by in vitro oxidations: characterization and mechanisms of formation. Journal of Agricultural and Food Chemistry, 51, 7318–7325.

    Article  CAS  Google Scholar 

  • Carlson, B. L., Knorr, D., & Watkins, T. R. (1981). Influence of tomato seed addition on the quality of the wheat flour breads. Journal of Food Science, 46, 1029–1031.

    Article  Google Scholar 

  • Chen, B., McClements, D. J., & Decker, E. A. (2011). Minor components in food oils: a critical review of their roles on lipid oxidation chemistry in bulk oils and emulsions. Critical Reviews in Food Science and Nutrition, 51, 901–916.

    Article  CAS  Google Scholar 

  • Choe, E., & Min, D. B. (2009). Mechanisms of antioxidants in the oxidation of foods. Comprehensive Reviews in Food Science and Food Safety, 8, 345–358.

    Article  CAS  Google Scholar 

  • Codex Alimentarius Commission. (2005). CODEX standard for named vegetable oils, CODEX STAN 210. Rome: Food and Agriculture Organisation of the United Nations.

    Google Scholar 

  • Del Valle, M., Cámara, M., & Torija, M. E. (2006). Chemical characterization of tomato pomace. Journal of the Science of Food and Agriculture, 86, 1232–1236.

    Article  Google Scholar 

  • Demirbas, A. (2010). Oil, micronutrient and heavy metal contents of tomatoes. Food Chemistry, 118, 504–507.

    Article  CAS  Google Scholar 

  • Di Mascio, P., Kaiser, S., & Sies, H. (1989). Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Archives of Biochemistry and Biophysics, 274, 532–538.

    Article  Google Scholar 

  • Ekthamasut, K. (2006). Effect of tomato seed meal on wheat pasting properties and alkaline noodle qualities. Australian Journal of Technology, 9, 147–152.

    Google Scholar 

  • Eller, F. J., Moser, J. K., Kenar, J. A., & Taylor, S. L. (2010). Extraction and analysis of tomato seed oil. Journal of the American Oil Chemists' Society, 87, 755–762.

    Article  CAS  Google Scholar 

  • Fielding, J. M., Rowley, K. G., Cooper, P., & O’Dea, K. (2005). Increases in plasma lycopene concentration after consumption of tomatoes cooked with olive oil. Asia Pacific Journal of Clinical Nutrition, 14, 131–136.

    CAS  Google Scholar 

  • Fish, W. W., Perkins-Veazie, P., & Collins, J. K. (2002). A quantitative assay for lycopene that utilizes reduced volumes of organic solvents. Journal of Food Composition and Analysis, 15, 309–317.

    Article  CAS  Google Scholar 

  • Giannelos, P. N., Sxizas, S., Lois, E., Zannikos, F., & Anastopoulos, G. (2005). Physical, chemical and fuel related properties of tomato seed oil for evaluating its direct use in diesel engines. Industrial Crops and Products, 22, 193–199.

    Article  CAS  Google Scholar 

  • González, S., Fernandez-Lorente, M., & Gilaberte-Calzada, Y. (2008). The latest on skin photoprotection. Clinics in Dermatology, 26, 614–616.

    Article  Google Scholar 

  • Hetzroni, A., Vana, A., & Mizrach, A. (2011). Biomechanical characteristics of tomato fruit peels. Postharvest Biology and Technology, 59, 80–84.

    Article  Google Scholar 

  • Kelkel, M., Schumacher, M., Dicato, M., & Diederich, M. (2011). Antioxidant and anti-proliferative properties of lycopene. Free Radical Research, 45, 925–940.

    Article  CAS  Google Scholar 

  • Khachik, F., Carvalho, L., Bernstein, P. S., Muir, G. J., Zhao, D. Y., & Katz, N. B. (2002). Chemistry, distribution, and metabolism of tomato carotenoids and their impact on human health. Experimental Biology and Medicine, 227, 845–851.

    CAS  Google Scholar 

  • Kochhar, S. P. (2000). Stabilisation of frying oils with natural antioxidative components. European Journal of Lipid Science and Technology, 102, 552–559.

    Article  CAS  Google Scholar 

  • Köckritz, A., & Martin, A. (2008). Oxidation of unsaturated fatty acid derivatives and vegetable oils. European Journal of Lipid Science and Technology, 110, 812–824.

    Article  Google Scholar 

  • Kong, K. W., Khoo, H. E., Prasad, K. N., Ismail, A., Tan, C. P., & Rajab, N. F. (2010). Revealing the power of the natural red pigment lycopene. Molecules, 15, 959–987.

    Article  CAS  Google Scholar 

  • Kucuk, O., Sarkar, F. H., Sakr, W., Djuric, Z., Pollak, M. N., Khachik, F., et al. (2001). Phase II randomized clinical trial of lycopene supplementation before radical prostatectomy. Cancer Epidemiology, Biomarkers & Prevention, 10, 861–868.

    CAS  Google Scholar 

  • Lavecchia, R., & Zuorro, A. (2008). Improved lycopene extraction from tomato peels using cell-wall degrading enzymes. European Food Research and Technology, 228, 153–158.

    Article  CAS  Google Scholar 

  • Lazos, E. S., Tsaknis, J., & Lalas, S. (1998). Characteristics and composition of tomato seed oil. Grasas y Aceites, 49, 440–445.

    Article  CAS  Google Scholar 

  • Leclercq, S., Reineccius, G. A., & Milo, C. (2007). Effect of type of oil and addition of δ-tocopherol on model flavor compound stability during storage. Journal of Agricultural and Food Chemistry, 55, 9189–9194.

    Article  CAS  Google Scholar 

  • Loizou, S., Lekakis, I., Chrousos, G. P., & Moutsatsou, P. (2010). Beta-sitosterol exhibits anti-inflammatory activity in human aortic endothelial cells. Molecular Nutrition & Food Research, 54, 551–558.

    Article  CAS  Google Scholar 

  • Longo, C., Leo, L., & Leone, A. (2012). Carotenoids, fatty acid composition and heat stability of supercritical carbon dioxide-extracted-oleoresins. International Journal of Molecular Sciences, 13, 4233–4254.

    Article  CAS  Google Scholar 

  • Majzoobi, M., Ghavi, F. S., Farahnaky, A., Jamalian, J., & Mesbahi, G. (2011). Effect of tomato pomace powder on the physicochemical properties of flat bread (Barbari bread). Journal of Food Processing and Preservation, 35, 247–256.

    Article  CAS  Google Scholar 

  • Myers, R. H., & Montgomery, D. C. (1995). Response surface methodology. Process and product optimization using designed experiments. New York: Wiley.

    Google Scholar 

  • Offord, E. A., Gautier, J. C., Avanti, O., Scaletta, C., Runge, F., Krämer, K., et al. (2002). Photoprotective potential of lycopene, β-carotene, vitamin E, vitamin C and carnosic acid in UVA-irradiated human skin fibroblasts. Free Radical Biology & Medicine, 32, 1293–1303.

    Article  CAS  Google Scholar 

  • Omoni, A. O., & Aluko, R. E. (2005). The anti-carcinogenic and anti-atherogenic effects of lycopene: a review. Trends in Food Science and Technology, 16, 344–350.

    Article  CAS  Google Scholar 

  • Park, C., Moon, D., Rhu, C. H., Choi, B. T., Lee, W. H., Kim, G. Y., et al. (2007). β-Sitosterol induces anti-proliferation and apoptosis in human leukemic U937 cells through activation of caspase-3 and induction of Bax/Bcl-2 ratio. Biological & Pharmaceutical Bulletin, 30, 1317–1323.

    Article  CAS  Google Scholar 

  • Rao, A. V., & Ali, A. (2007). Biologically active phytochemicals in human health: lycopene. International Journal of Food Properties, 10, 279–288.

    Article  CAS  Google Scholar 

  • Rao, A. V., & Shen, H. (2002). Effect of low dose lycopene intake on lycopene bioavailability and oxidative stress. Nutrition Research, 22, 1125–1131.

    Article  CAS  Google Scholar 

  • Rao, A. V., Raym, M. R., & Rao, L. G. (2006). Lycopene. Advances in Food and Nutrition Research, 51, 99–164.

    Article  CAS  Google Scholar 

  • Riccioni, G., Mancini, B., Di Ilio, E., Bucciarelli, T., & D’Orazio, N. (2008). Protective effect of lycopene in cardiovascular disease. European Review for Medical and Pharmacological Sciences, 12, 183–190.

    CAS  Google Scholar 

  • Rittié, L., & Fisher, G. J. (2002). UV-light-induced signal cascades and skin aging. Ageing Research Reviews, 1, 705–720.

    Article  Google Scholar 

  • Roy, B. C., Goto, M., & Hirose, T. (1996). Temperature and pressure effects on supercritical CO2 extraction of tomato seed oil. International Journal of Food Science and Technology, 31, 137–141.

    Article  CAS  Google Scholar 

  • Rozzi, N. L., Singh, R. K., Vierling, R. A., & Watkins, B. A. (2002). Supercritical fluid extraction of lycopene from tomato processing by-products. Journal of Agricultural and Food Chemistry, 50, 2638–2643.

    Article  CAS  Google Scholar 

  • Ryan, L., Mestrallet, M. G., Nepote, V., Conci, S., & Grosso, N. R. (2008). Composition, stability and acceptability of different vegetable oils used for frying peanuts. International Journal of Food Science and Technology, 43, 193–199.

    CAS  Google Scholar 

  • Sabio, E., Lozano, M., Montero de Espinosa, V., Mendes, R. L., Pereira, A. P., Palavra, A. F., et al. (2003). Lycopene and β-carotene extraction from tomato processing waste using supercritical CO2. Industrial and Engineering Chemistry Research, 42, 6641–6646.

    Article  CAS  Google Scholar 

  • Seren, S., Lieberman, R., Bayraktar, U. D., Heath, E., Sahin, K., Andic, F., et al. (2008). Lycopene in cancer prevention and treatment. American Journal of Therapeutics, 15, 66–81.

    Article  Google Scholar 

  • Sharma, S. K., & LeMaguer, M. (1996). Lycopene in tomatoes and tomato pulp fractions. Italian Journal of Food Science, 2, 107–113.

    Google Scholar 

  • Shen, X., & Xu, S. (2005). Supercritical CO2 extraction of tomato seed oil. Journal of Food Technology, 3, 226–231.

    Google Scholar 

  • Shi, J., LeMaguer, M., Mazza, G. (2002). Lycopene from tomatoes. In Functional foods (pp. 135–167). Boca Raton: CRC.

  • Shixian, Q., Dai, Y., Kakuda, Y., Shi, J., Mittal, G., Yeung, D., et al. (2005). Synergistic anti-oxidative effects of lycopene with other bioactive compounds. Food Reviews International, 21, 295–311.

    Article  CAS  Google Scholar 

  • Sies, H., & Stahl, W. (1998). Lycopene: antioxidant and biological effects and its bioavailability in the human. Proceedings of the Society for Experimental Biology and Medicine, 218, 121–124.

    Article  CAS  Google Scholar 

  • Sogi, D. S., Sidhu, J. S., Arora, M. S., Garg, S. K., & Bawa, A. S. (2002). Effect of tomato seed meal supplementation on dough and bread characteristics of wheat (PBW-343) flour. International Journal of Food Properties, 5, 563–571.

    Article  CAS  Google Scholar 

  • Spanos, G. A., Chen, H., & Schwartz, S. J. (1992). Supercritical CO2 extraction of β-carotene from sweet potatoes. Journal of Food Science, 38, 817–820.

    Google Scholar 

  • Stahl, W., Heinrich, U., Aust, O., Tronnier, H., & Sies, H. (2006). Lycopene-rich products and dietary photoprotection. Photochemical & Photobiological Sciences, 5, 238–242.

    Article  CAS  Google Scholar 

  • Strati, I. F., & Oreopoulou, V. (2011). Effect of extraction parameters on the carotenoid recovery from tomato waste. International Journal of Food Science and Technology, 46, 23–29.

    Article  CAS  Google Scholar 

  • van het Hof, K. H., West, C. E., Weststrate, J. A., & Hautvast, J. G. A. J. (2000). Dietary factors that affect the bioavailability of carotenoids. Journal of Nutrition, 130, 503–506.

    Google Scholar 

  • Van Hoed, V. (2010). Phenolic compounds in seed oil. Lipid Technology, 22, 247–249.

    Article  Google Scholar 

  • WPTC (2011). World production estimate as of 21 October 2011, Release # 38. http://www.wptc.to/releases/releases38.pdf. Accessed 18 June 2012.

  • Xianquan, S., Shi, J., Kakuda, Y., & Yueming, J. (2005). Stability of lycopene during food processing and storage. Journal of Medicinal Food, 8, 413–422.

    Article  CAS  Google Scholar 

  • Zhao, Y., Chang, S. K., Qu, G., Li, T., & Cui, H. (2009). Beta-sitosterol inhibits cell growth and induces apoptosis in SGC-7901 human stomach cancer cells. Journal of Agricultural and Food Chemistry, 24, 5211–5218.

    Article  Google Scholar 

  • Zuorro, A., & Lavecchia, R. (2010). Mild enzymatic method for the extraction of lycopene from tomato paste. Biotechnology and Biotechnological Equipment, 24, 1854–1857.

    Article  CAS  Google Scholar 

  • Zuorro, A., Fidaleo, M., & Lavecchia, R. (2011). Enzyme-assisted extraction of lycopene from tomato processing waste. Enzyme and Microbial Technology, 49, 567–573.

    Article  CAS  Google Scholar 

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Correspondence to Roberto Lavecchia.

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Zuorro, A., Lavecchia, R., Medici, F. et al. Enzyme-Assisted Production of Tomato Seed Oil Enriched with Lycopene from Tomato Pomace. Food Bioprocess Technol 6, 3499–3509 (2013). https://doi.org/10.1007/s11947-012-1003-6

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