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Validation of a Novel Sequential Cultivation Method for the Production of Enzymatic Cocktails from Trichoderma Strains

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Abstract

The development of new cost-effective bioprocesses for the production of cellulolytic enzymes is needed in order to ensure that the conversion of biomass becomes economically viable. The aim of this study was to determine whether a novel sequential solid-state and submerged fermentation method (SF) could be validated for different strains of the Trichoderma genus. Cultivation of the Trichoderma reesei Rut-C30 reference strain under SF using sugarcane bagasse as substrate was shown to be favorable for endoglucanase (EGase) production, resulting in up to 4.2-fold improvement compared with conventional submerged fermentation. Characterization of the enzymes in terms of the optimum pH and temperature for EGase activity and comparison of the hydrolysis profiles obtained using a synthetic substrate did not reveal any qualitative differences among the different cultivation conditions investigated. However, the thermostability of the EGase was influenced by the type of carbon source and cultivation system. All three strains of Trichoderma tested (T. reesei Rut-C30, Trichoderma harzianum, and Trichoderma sp INPA 666) achieved higher enzymatic productivity when cultivated under SF, hence validating the proposed SF method for use with different Trichoderma strains. The results suggest that this bioprocess configuration is a very promising development for the cellulosic biofuels industry.

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Acknowledgments

The authors thank the Brazilian agencies Fapesp, Capes, and CNPq for financial support, and the staff of Embrapa Instrumentation for their technical assistance.

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Authors and Affiliations

  1. Graduate Program of Biotechnology, Federal University of São Carlos, 13565-905, São Carlos, SP, Brazil

    C. Florencio, A. C. Badino & C. S. Farinas

  2. Graduate Program of Chemical Engineering, Federal University of São Carlos, C.P. 676, 13565-905, São Carlos, SP, Brazil

    F. M. Cunha, A. C. Badino & C. S. Farinas

  3. Embrapa Instrumentation, Rua XV de Novembro 1452, 13560-970, São Carlos, SP, Brazil

    C. Florencio, F. M. Cunha & C. S. Farinas

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  1. C. Florencio
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  2. F. M. Cunha
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  4. C. S. Farinas
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Correspondence to C. S. Farinas.

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Florencio, C., Cunha, F.M., Badino, A.C. et al. Validation of a Novel Sequential Cultivation Method for the Production of Enzymatic Cocktails from Trichoderma Strains. Appl Biochem Biotechnol 175, 1389–1402 (2015). https://doi.org/10.1007/s12010-014-1357-5

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  • DOI: https://doi.org/10.1007/s12010-014-1357-5

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