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Morphological, Histobiochemical and Molecular Characterisation of Low Lignin Phloem Fibre (llpf) Mutant of Dark Jute (Corchorus olitorius L.)

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Abstract

Lignin is a versatile plant metabolite challenging high-end industrial applications of several plant products including jute. Application of developmental mutant in regulation of lignification in jute may open up door for much awaited jute based diversified products. In the present study, a novel dark jute (Corchorus olitorius L.) mutant with low lignin (7.23%) in phloem fibre being compared to wild-type JRO 204 (13.7%) was identified and characterised. Unique morphological features including undulated stem, petiole and leaf vein distinguished the mutant in gamma ray irradiated mutant population. Histological and biochemical analysis revealed reduced lignification of phloem fibre cells of the plant. RT-PCR analysis demonstrated temporal transcriptional regulation of CCoAMT1 gene in the mutant. The mutant was found an extremely useful model to study phloem fibre developmental biology in the crop besides acting as a donor genetic stock for low lignin containing jute fibre in dark jute improvement programme.

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References

  1. Karmakar, P. G., Hazra, S. K., Sinha, M. K., & Chaudhury, S. K. (2008). Breeding for quantitative traits and varietal development in jute and allied fibre crops. In P. G. Karmakar, S. K. Hazra, T. Ramasubramanian, R. K. Mandal, M. K. Sinha, & H. S. Sen (Eds.), Jute and allied fibre updates: production and technology (pp. 18–37). Barrackpore: Central Research Institute for Jute and Allied Fibres.

    Google Scholar 

  2. Summerscales, J., Dissanayake, N. P. J., Virk, A. S., & Hall, W. (2010). A review of bast fibres and their composites. Part 1—fibres as reinforcements. Composites Part A Applied Science and Manufacturing, 41, 1329–1335. doi:10.1016/j.compositesa.2010.06.001.

    Article  Google Scholar 

  3. Rio, J. C., Del, R. J., Marques, G., Li, J., Gellerstedt, G., Barbero, J. J., Martinez, A. T., & Gutierrez, A. (2009). Structural characterization of the lignin from jute (Corchorus capsularis) fibers. Journal of Agricultural and Food Chemistry, 57, 10271–10281. doi:10.1021/jf900815x.

    Article  Google Scholar 

  4. Kundu, B. C. (1944). Anatomy of jute stem with special reference to cambial activity and distribution of fibres in relation to leaf-trace system. J Royal Asiatic Soc Bengal (Sci)., 10, 27–52.

    Google Scholar 

  5. Kundu, B. C., Basak, K. C., & Sarkar, P. B. (1959). Jute in India. Calcutta: The Indian Central Jute Committee.

    Google Scholar 

  6. Maiti, R. K. (1997). World fibre crops. New Delhi: Oxford and IBH Publishing Company.

    Google Scholar 

  7. Hazra, S. K., & Karmakar, P. G. (2008). Anatomical parameters of bast fibres for yield and quality improvement. In P. G. Karmakar, S. K. Hazra, T. Ramasubramanian, R. K. Mandal, M. K. Sinha, & H. S. Sen (Eds.), Jute and allied fibre updates (pp. 38–45). Kolkata: Central Research Institute for Jute and Allied Fibres.

    Google Scholar 

  8. Day, A., Ruel, K., Neutelings, G., Cronier, D., David, H., Hawkins, S., & Chabbert, B. (2005). Lignification in the flax stem: evidence for an unusual lignin in bast fibres. Planta, 222, 234–245. doi:10.1007/s00425-005-1537-1.

    Article  CAS  Google Scholar 

  9. Rowell, R. M., & Stout, H. P. (2007). Jute and kenaf. In M. Lewin (Ed.), Handbook of fibre chemistry (3rd ed., pp. 405–452). Boca Raton: CRC Press.

    Google Scholar 

  10. Maiti, R. K., & Mitra, G. C. (1972). Cambial activity in relation to the production of fibre bundles at different phases of growth in white jute. Bull Bot Soc Bengal, 26, 79–85.

    Google Scholar 

  11. Mitra, G. C. (1984). Genetic control of secondary phloic fibres in jute (Corchorus capsularis L.) Genetica, 63, 9–11.

    Article  Google Scholar 

  12. Palit, P., & Meshram, J. H. (2004). Physiological characterization of a phenotypically distinct jute (Corchorus olitorius) genotype. Plant Genet. Resour., 2, 175–180.

    Article  Google Scholar 

  13. Sengupta, G., & Palit, P. (2004). Characterization of a lignified secondary phloem fibre-deficient mutant of jute (Corchorus capsularis). Annals of Botany, 93, 211–220. doi:10.1093/aob/mch029.

    Article  Google Scholar 

  14. Meshram, J. H., & Palit, P. (2013). On the role of cell wall lignin in determining the fineness of jute fibre. Acta Physiologiae Plantarum, 35, 1565–1578.

    Article  CAS  Google Scholar 

  15. Campbell, M. M., & Sederoff, R. R. (1996). Variation in lignin content and composition. Mechanisms of control and implications for the genetic improvement of plants. Plant Physiology, 110, 3–13.

    Article  CAS  Google Scholar 

  16. Wagner, G. P., & Lynch, V. J. (2008). The gene regulatory logic of transcription factor evolution. Trends in Ecology & Evolution, 23, 377–385. doi:10.1016/j.tree.2008.03.006.

    Article  Google Scholar 

  17. Ralph, J., Hatfield, R. D., Piquemal, J., Yahiaoui, N., & Pean, M. (1998). NMR characterization of altered lignins extracted from tobacco plants down regulated for lignification enzymes cinnamyl-alcohol dehydrogenase and cinnamoyl-CoA reductase. Proceedings of the National Academy of Sciences of the United States of America, 95, 12803–12808. doi:10.1073/pnas.95.22.12803.

    Article  CAS  Google Scholar 

  18. Ralph, J., Kim, H., Peng, J., & Lu, F. (1999). Arylpropane-1,3-diols in lignins from normal and CAD-deficient pines. Organic Letters, 1, 323–326. doi:10.1021/ol9906559.

    Article  CAS  Google Scholar 

  19. Ralph, J., Lapierre, C., Lu, F., Marita, J. M., & Pilate, G. (2001). NMR evidence for benzodioxane structures resulting from incorporation of 5-hydroxyconiferyl alcohol into lignins of O-methyltransferase-deficient plants. Journal of Agricultural and Food Chemistry, 49, 86–91. doi:10.1021/jf001042.

    Article  CAS  Google Scholar 

  20. Provan, G. J., Scobbie, L., & Chesson, A. (1997). Characterisation of lignin from CAD and OMT deficient bm mutants of maize. Journal of the Science of Food and Agriculture, 73, 133–142. doi:10.1002/(SICI)1097-0010(199702)73:2<133::AID-JSFA696>3.0.CO;2-Q.

    Article  CAS  Google Scholar 

  21. Piquemal, J., Chamayou, S., Nadaud, I., Beckert, M., & Barriere, Y. (2002). Down-regulation of caffeic acid omethyltransferase in maize revisited using a transgenic approach. Plant Physiology, 130, 1675–1685. doi:10.1104/pp.012237.

    Article  CAS  Google Scholar 

  22. Jones, L., Ennos, A. R., & Turner, S. R. (2001). Cloning and characterization of irregular xylem4 (irx4): a severely lignin-deficient mutant of Arabidopsis. The Plant Journal, 26, 205–216. doi:10.1046/j.1365-313x.2001.01021.x.

    Article  CAS  Google Scholar 

  23. Leplé, J. C., Dauwe, R., Morreel, K., Storme, V., Lapierre, C., Pollet, B., Naumann, A., & Leroux, O. (2012). Collenchyma: a versatile mechanical tissue with dynamic cell walls. Annals of Botany, 110, 1083–1098. doi:10.1093/aob/mcs186.

    Article  Google Scholar 

  24. Zhou, R., Jackson, L., Shadle, G., Nakashima, J., Temple, S., Chen, F., & Dixon, R. A. (2010). Distinct cinnamoyl CoA reductases involved in parallel routes to lignin in Medicago truncatula. Proceedings of the National Academy of Sciences of the United States of America, 107, 17803–17808. doi:10.1073/pnas.1012900107.

    Article  CAS  Google Scholar 

  25. Rogers, L. A., & Campbell, M. M. (2004). The genetic control of lignin deposition during plant growth and development. The New Phytologist, 164, 17–30. doi:10.1111/j.1469-8137.2004.01143.x.

    Article  CAS  Google Scholar 

  26. Bandopadhyay, S. B., & Mukhopadhyay, S. K. (1964). Assessment of jute fibre bundle strength. Jute Bull., 27, 193–199.

    Google Scholar 

  27. Sinha, N. G., & Bandopadhyay, S. B. (1968). An air-flow method for the determination of the fibre fineness of jute and mesta. Journal of the Textile Institute, 59, 148–156.

    Article  Google Scholar 

  28. Kundu, A., Sarkar, D., Mandal, N. A., Sinha, M. K., & Mahapatra, B. S. (2012). A secondary phloic (bast) fibre-shy (bfs) mutant of dark jute (Corchorus olitorius L.) develops lignified fibre cells but is defective in cambial activity. Plant Growth Regulation, 67(1), 45–55. doi:10.1007/s10725-012-9660-z.

    Article  CAS  Google Scholar 

  29. Sengupta, A. B., Mazumdar, S. K., & Macmillan, N. G. (1958). Isolation of jute holocellulose by the action of sodium chlorite. Indian J Appl Chem., 21(10), 105.

    CAS  Google Scholar 

  30. Dashek, W. V. (1997). Methods in plant biochemistry and molecular biology. Boca Raton: CRC Press.

    Google Scholar 

  31. Inc, S. P. S. S. Released 2007. SPSS for Windows, version 16.0. Chicago: SPSS Inc..

  32. Kundu, A., Sarkar, D., Bhattacharjee, A., Topdar, N., Sinha, M. K., & Mahapatra, B. S. (2011). A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages. Electronic Journal of Biotechnology, 14(1). doi:10.2225/vol14-issue1-fulltext-4.

  33. Choudhary, S. B., Kumar, M., Chowdhury, I., Singh, R. K., Pandey, S. P., Sharma, H. K., & Karmakar, P. G. (2016). An efficient and cost effective method of RNA extraction from mucilage, phenol and secondary metabolite rich bark tissue of tossa jute (C. olitorius L.) actively developing phloem fiber. 3Biotech, 6, 100. doi:10.1007/s13205-016-0415-9.

    CAS  Google Scholar 

  34. Zhang, G., Zhang, Y., Xu, J., Niu, X., Qi, J., Tao, A., Zhang, L., Fang, P., Lin, L., & Hui, J. S. (2014). The CCoAOMT1 gene from jute (Corchorus capsularis L.) is involved in lignin biosynthesis in Arabidopsis thaliana. Gene, 546, 398–402.

    Article  CAS  Google Scholar 

  35. Chakraborty, A., Sarkar, D., Satya, P., Karmakar, P. G., & Singh, N. K. (2015). Pathways associated with lignin biosynthesis in lignomaniac jute fibres. Molecular Genetics and Genomics, 290, 1523–1542. doi:10.1007/s00438-015-1013-y.

    Article  CAS  Google Scholar 

  36. Lewis, N. G., & Yamamoto, E. (1990). Lignin: Occurrence, biogenesis and biodegradation. Annual Review of Plant Physiology and Plant Molecular Biology, 41, 455–496. doi:10.1146/annurev.pp.41.060190.002323.

    Article  CAS  Google Scholar 

  37. Kokubo, A., Kuraishi, S., & Sakurai, N. (1989). Culm strength of barley: correlation among maximum bending stress, cell wall dimensions, and cellulose content. Plant Physiology, 91, 876–882.

    Article  CAS  Google Scholar 

  38. Kokubo, A., Sakurai, N., Kuraishi, S., & Takabe, K. (1991). Culm brittleness of barley (Hordeum vulgare L.) mutants is caused by smaller number of cellulose molecules in cell wall. Plant Physiology, 97, 509–514. doi:10.1104/pp.91.3.876.

    Article  CAS  Google Scholar 

  39. Qian, Q., Li, Y. H., Zeng, D., Teng, S., Wang, Z., Li, X., Dong, Z., Dai, N., Sun, L., & Li, J. (2001). Isolation and genetic characterization of a fragile plant mutant rice (Oryza sativa L.) Chinese Science Bulletin, 46, 2082–2085. doi:10.1007/BF02901137.

    Article  CAS  Google Scholar 

  40. Snegireva, A., Tatyana, C., Marina, A., Simcha, L., & Tatyana, G. (2015). Intrusive growth of primary and secondary phloem fibres in hemp stem determines fibre bundle formation and structure. AoB PLANTS. doi:10.1093/aobpla/plv061.

    Google Scholar 

  41. Meshitsuka, G., & Nakano, J. (1979). Studies on the mechanism of lignin color reaction (XIII): Maule color reaction (9). Mokuzai Gakkaishi, 25, 588–594.

    CAS  Google Scholar 

  42. Zhao, H. Y. (2005). Isolation and functional characterisation of cinamate-4 hydroxylase promoter from Populous tomentosa L. Plant Science, 168, 1157–1162. doi:10.1016/j.plantsci.2004.12.022.

    Article  CAS  Google Scholar 

  43. Mele, G., Orie, N., Sato, Y., & Hake, S. (2003). The knotted-1 like homeobox gene BREVIPEDICELLUS regulates cell differentiation by modulating metabolic pathways. Genes & Development, 17, 2088–2093. doi:10.1101/gad.1120003.

    Article  CAS  Google Scholar 

  44. Liljegren, S. J., Ditta, G. S., Eshed, H. Y., Savidge, B., Bowman, J. L., & Yanofsky, M. F. (2000). Shatter proof MADS-box genes control seed dispersal in Arabidopsis. Nature, 404(6779), 766–770. doi:10.1038/35008089.

    Article  CAS  Google Scholar 

  45. Zhong, R., Burk, D. H., Morrison, W. H., & Ye, Z. H. (2002). A kinesin like protein is essential for oriented deposition of cellulose microfibrils and cell wall strength. Plant Cell, 14, 3101–3117. doi:10.1105/tpc.005801.

    Article  CAS  Google Scholar 

  46. Bell-Lelong, D. A., Cusumano, J. C., Meyer, K., & Chapple, C. (1997). Cinnamate-4-hydroxylase expression in Arabidopsis (regulation in response to development and the environment). Plant Physiology, 113(3), 729–738. doi:10.1104/pp.113.3.729.

    Article  CAS  Google Scholar 

  47. Cabane, M., Pireaux, J. C., Leger, E., Weber, E., Dizengremel, P., Pollet, B., & Lapierre, C. (2004). Condensed lignins are synthesized in poplar leaves exposed to ozone. Plant Physiology, 134, 586–594. doi:10.1104/pp.103.031765.

    Article  CAS  Google Scholar 

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Acknowledgements

The authors acknowledge the financial assistance for the work received in the form of research grant (35/14/01/2014-BRNS) from BRNS, Department of Atomic Energy, Govt. of India. Valuable suggestions given by Dr. D. Sarkar, Pr. Scientist, ICAR-CRIJAF, for data presentation and manuscript writing are deeply acknowledged.

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Authors and Affiliations

  1. ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata, West Bengal, 700120, India

    S. B. Choudhary, I. Chowdhury, H. K. Sharma, A. Anil Kumar & P. G. Karmakar

  2. Indian Institute of Science Education and Research Kolkata, Mohanpur, West Bengal, 741246, India

    R. K. Singh & S. P. Pandey

  3. Banaras Hindu University-Institute of Agricultural Science, Varanasi, U.P, 221005, India

    N. Kumari

  4. Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India

    J. Souframanien & S. J. Jambhulkar

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  1. S. B. Choudhary
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  2. I. Chowdhury
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Electronic Supplementary Material

Supplementary file 1

Phenotype of wild type JRO 204 plant, stem, leaf, root (a-d) and llpf mutant plant, stem, leaf, root (e-h). (GIF 518 kb)

High resolution image (TIFF 2797 kb)

Supplementary file 2

PCR analysis for CCoAMT1 gene using genomic DNA. 100 bp DNA ladder (a), wild type JRO 204 (b), llpf mutant (c). (GIF 763 kb)

High resolution image (TIFF 1022 kb)

Supplementary file 3

RT-PCR analysis for CCoAMT1 gene. 100 bp DNA ladder (a), wild-type JRO 204 at 20 DAS (b), 45 DAS (d), 70 DAS (f) and llpf mutant at 20 DAS (c), 45 DAS (e), 70 DAS (g). (GIF 5993 kb)

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Choudhary, S.B., Chowdhury, I., Singh, R.K. et al. Morphological, Histobiochemical and Molecular Characterisation of Low Lignin Phloem Fibre (llpf) Mutant of Dark Jute (Corchorus olitorius L.). Appl Biochem Biotechnol 183, 980–992 (2017). https://doi.org/10.1007/s12010-017-2477-5

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