Skip to main content
SpringerLink
Log in

Antimicrobial and Antiviral Activities of Durable Cotton Fabrics Treated with Nanocomposite Based on Zinc Oxide Nanoparticles, Acyclovir, Nanochitosan, and Clove Oil

  • Original Article
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

In this study, cotton fabrics based on zinc oxide nanoparticles in situ synthesis, acyclovir, nanochitosan, and clove oil were treated. The treated cotton fabrics were examined by FTIR, HR-TEM, FE-SEM, EDAX, and the surface roughness processing of FE-SEM images. The obtained characterization data emphasized the nano-size of nanocomposite with high homogeneity of particles in spherical shape as well as affirmed the deposition of nanocomposite onto the textile fibers with concluded that the deposition of nanocomposite was increased parallel with sonication time. Antimicrobial and antiviral activities of treated cotton fabrics were evaluated. Results revealed that treated cotton fabrics exhibited promising antibacterial activity toward Gram-positive higher than Gram-negative bacteria. Likewise, treated cotton fabrics are still effective as antibacterial after washing for 100 cycles. Moreover, treated cotton fabrics exhibited potential antifungal activity against Candida albicans, Aspergillus niger, and Aspergillus fumigatus. The antiviral activity significantly depended on the type of virus. The treated cotton fabrics showed antiviral activity against tested viral particles (HSV-1, Adeno, and CoxB2) with viral inhibition of 95.9, 76.4, and 86.9% respectively, while in the case of coated cotton textile with acyclovir, it only exhibited viral inhibition of 49.9, 41, and 22.3% respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Code Availability

Not applicable.

References

  1. Abdelaziz, A. M., et al. (2021). Protective role of zinc oxide nanoparticles based hydrogel against wilt disease of pepper plant Biocatalysis and Agricultural. Biotechnology, 35, 102083. https://doi.org/10.1016/j.bcab.2021.102083

    Article  CAS  Google Scholar 

  2. Abu-Elghait, M., Hasanin, M., Hashem, A. H., & Salem, S. S. (2021). Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility. International Journal of Biological Macromolecules, 175, 294–303. https://doi.org/10.1016/j.ijbiomac.2021.02.040

    Article  PubMed  CAS  Google Scholar 

  3. Anand, M., Sathyapriya, P., Maruthupandy, M., & HameedhaBeevi, A. (2018). Synthesis of chitosan nanoparticles by TPP and their potential mosquito larvicidal application. Frontiers in Laboratory Medicine, 2, 72–78. https://doi.org/10.1016/j.flm.2018.07.003

    Article  Google Scholar 

  4. Applerot, G., Lipovsky, A., Dror, R., Perkas, N., Nitzan, Y., Lubart, R., & Gedanken, A. (2009). Enhanced antibacterial activity of nanocrystalline ZnO due to increased ROS-mediated cell injury. Advanced Functional Materials, 19, 842–852.

    Article  CAS  Google Scholar 

  5. Baranwal, A., Srivastava, A., Kumar, P., Bajpai, V. K., Maurya, P. K., & Chandra, P. (2018). Prospects of nanostructure materials and their composites as antimicrobial agents. Frontiers in microbiology, 9, 422.

    Article  Google Scholar 

  6. Dacrory, S., Hashem, A. H., & Hasanin, M. (2021). Synthesis of cellulose based amino acid functionalized nano-biocomplex: Characterization, antifungal activity, molecular docking and hemocompatibility Environmental Nanotechnology. Monitoring & Management, 15, 100453. https://doi.org/10.1016/j.enmm.2021.100453

    Article  Google Scholar 

  7. Dalirfardouei, R., Karimi, G., & Jamialahmadi, K. (2016). Molecular mechanisms and biomedical applications of glucosamine as a potential multifunctional therapeutic agent. Life sciences, 152, 21–29.

    Article  CAS  Google Scholar 

  8. El-Naggar, M. E., Abdel-Aty, A. M., Wassel, A. R., Elaraby, N. M., & Mohamed, S. A. (2021). Immobilization of horseradish peroxidase on cationic microporous starch: Physico-bio-chemical characterization and removal of phenolic compounds. International Journal of Biological Macromolecules, 181, 734–742.

    Article  CAS  Google Scholar 

  9. Elbasuney, S., El-Sayyad, G. S., Tantawy, H., & Hashem, A. H. (2021). Promising antimicrobial and antibiofilm activities of reduced graphene oxide-metal oxide (RGO-NiO, RGO-AgO, and RGO-ZnO) nanocomposites. RSC Advances, 11(42), 25961-25975.‏

  10. Elbahnasawy MA, Shehabeldine AM, Khattab AM, Amin BH, Hashem AH (2021) Green biosynthesis of silver nanoparticles using novel endophytic Rothia endophytica: Characterization and anticandidal activity Journal of Drug Delivery Science and Technology:102401

  11. Erkoc, P., & Ulucan-Karnak, F. (2021). Nanotechnology-based antimicrobial and antiviral surface coating strategies. Prosthesis, 3, 25–52.

    Article  Google Scholar 

  12. Galdiero, S., Falanga, A., Vitiello, M., Cantisani, M., Marra, V., & Galdiero, M. (2011). Silver nanoparticles as potential antiviral agents. Molecules, 16, 8894–8918.

    Article  CAS  Google Scholar 

  13. George, S., et al. (2010). Use of a rapid cytotoxicity screening approach to engineer a safer zinc oxide nanoparticle through iron doping. ACS nano, 4, 15–29.

    Article  CAS  Google Scholar 

  14. Hanley, C., Thurber, A., Hanna, C., Punnoose, A., Zhang, J., & Wingett, D. G. (2009). The influences of cell type and ZnO nanoparticle size on immune cell cytotoxicity and cytokine induction. Nanoscale research letters, 4, 1409–1420.

    Article  CAS  Google Scholar 

  15. Haque M, McKimm J (2020) Strategies to Prevent Healthcare-Associated Infections: A Narrative Overview 13:1765-1780https://doi.org/10.2147/rmhp.s269315

  16. Hasanin, M., Hashem, A. H., El-Rashedy, A. A., & Kamel, S. (2021). Synthesis of novel heterocyclic compounds based on dialdehyde cellulose: Characterization, antimicrobial, antitumor activity, molecular dynamics simulation and target identification. Cellulose. https://doi.org/10.1007/s10570-021-04063-7

    Article  Google Scholar 

  17. Hasanin, M., Hassan, S. A., & Hashem, A. H. (2021). Green biosynthesis of zinc and selenium oxide nanoparticles using callus extract of Ziziphus spina-christi: characterization, antimicrobial, and antioxidant activity. Biomass Conversion and Biorefinery, 1-14.‏

  18. Hashem AH, Khalil AMA, Reyad AM, Salem SS (2021) Biomedical applications of mycosynthesized selenium nanoparticles using penicillium expansum ATTC 36200 Biological Trace Element Research:1–11

  19. Hashem, A. H., Saied, E., & Hasanin, M. S. (2020). Green and ecofriendly bio-removal of methylene blue dye from aqueous solution using biologically activated banana peel waste. Sustainable Chemistry and Pharmacy, 18, 100333.

    Article  Google Scholar 

  20. Hashem, A. H., Suleiman, W. B., Abu-elreesh, G., Shehabeldine, A. M., & Khalil, A. M. A. (2020). Sustainable lipid production from oleaginous fungus Syncephalastrum racemosum using synthetic and watermelon peel waste media. Bioresource Technology Reports, 12, 100569. https://doi.org/10.1016/j.biteb.2020.100569

    Article  Google Scholar 

  21. Hashem AH, Suleiman WB, Abu-Elrish GM, El-Sheikh HH (2020c) Consolidated bioprocessing of sugarcane bagasse to microbial oil by newly isolated oleaginous fungus: Mortierella wolfii Arabian Journal for Science and Engineering:1–13

  22. Hashem, A. H., Abdelaziz, A. M., Askar, A. A., Fouda, H. M., Khalil, A., Abd-Elsalam, K. A., & Khaleil, M. M. (2021). Bacillus megaterium-Mediated Synthesis of Selenium Nanoparticles and Their Antifungal Activity against Rhizoctonia solani in Faba Bean Plants. Journal of Fungi, 7(3), 195.‏

  23. He, L., Liu, Y., Mustapha, A., & Lin, M. (2011). Antifungal activity of zinc oxide nanoparticles against Botrytis cinerea and Penicillium expansum. Microbiological Research, 166, 207–215. https://doi.org/10.1016/j.micres.2010.03.003

    Article  PubMed  CAS  Google Scholar 

  24. Hebeish, A., El-Naggar, M., Fouda, M. M., Ramadan, M., Al-Deyab, S. S., & El-Rafie, M. (2011). Highly effective antibacterial textiles containing green synthesized silver nanoparticles. Carbohydrate Polymers, 86, 936–940.

    Article  CAS  Google Scholar 

  25. Juarez, D., Long, K. C., Aguilar, P., Kochel, T. J., & Halsey, E. S. (2013). Assessment of plaque assay methods for alphaviruses. Journal of virological methods, 187, 185–189. https://doi.org/10.1016/j.jviromet.2012.09.026

    Article  PubMed  CAS  Google Scholar 

  26. Kettley S (2011) The design of technical textiles. In: Textile Design. Elsevier, pp 323–353

  27. Khalil, A. M. A., & Hashem, A. H. (2018). Morphological changes of conidiogenesis in two aspergillus species J Pure. Applied Microbiology, 12, 2041–2048.

    CAS  Google Scholar 

  28. Krishnaveni, R., & Thambidurai, S. (2013). Industrial method of cotton fabric finishing with chitosan–ZnO composite for anti-bacterial and thermal stability. Industrial Crops and Products, 47, 160–167.

    Article  CAS  Google Scholar 

  29. Lee, S.-C., Lo, H.-J., Fung, C.-P., Lee, N., & See, L.-C. (2009). Disk diffusion test and E-test with enriched Mueller-Hinton agar for determining susceptibility of Candida species to voriconazole and fluconazole. Journal of microbiology, immunology, and infection= Wei mian yu gan ran za zhi, 42, 148–153.

    PubMed  CAS  Google Scholar 

  30. Nabi J, Akhter Y, Tabassum N (2020) Health care associated infections: A global threat epidemiology and transmission of infectious diseases:137

  31. Nagaraju G, Prashanth S, Shastri M, Yathish K, Anupama C, Rangappa DJMRB (2017) Electrochemical heavy metal detection, photocatalytic, photoluminescence, biodiesel production and antibacterial activities of Ag–ZnO nanomaterial 94:54-63

  32. Nga, N., Ngoc, T., Trinh, N., Thuoc, T., & Thao, D. (2020). Optimization and application of MTT assay in determining density of suspension cells. Analytical Biochemistry, 610, 113937.

    Article  CAS  Google Scholar 

  33. Noman, M. T., & Petrů, M. (2020). Functional properties of sonochemically synthesized zinc oxide nanoparticles and cotton composites. Nanomaterials, 10, 1661.

    Article  CAS  Google Scholar 

  34. Nugrahani I, Musaddah M (2016) Development and validation analysis of acyclovir tablet content determination method using FTIR Int J App Pharm 8:43-47

  35. Salama A, Hasanin M, Hesemann P (2020) Synthesis and antimicrobial properties of new chitosan derivatives containing guanidinium groups Carbohydrate Polymers:116363

  36. Sánchez-Bautista, A., Coy, J., García-Shimizu, P., & Rodríguez, J. C. (2018). From CLSI to EUCAST guidelines in the interpretation of antimicrobial susceptibility: What is the effect in our setting? Enfermedades infecciosas y microbiologia clinica (English ed), 36, 229–232.

    Google Scholar 

  37. Shehabeldine, A., & Hasanin, M. (2019). Green synthesis of hydrolyzed starch–chitosan nano-composite as drug delivery system to gram negative bacteria. Environmental Nanotechnology, Monitoring & Management, 12, 100252.

    Article  Google Scholar 

  38. Sotiriou, G. A., et al. (2014). Engineering safer-by-design silica-coated ZnO nanorods with reduced DNA damage potential Environmental Science. NANO, 1, 144–153.

    CAS  Google Scholar 

  39. Tavakoli, A., et al. (2018). Polyethylene glycol-coated zinc oxide nanoparticle: An efficient nanoweapon to fight against herpes simplex virus type 1. Nanomedicine, 13, 2675–2690.

    Article  CAS  Google Scholar 

  40. Tchouaket Nguemeleu E, Boivin S, Robins S, Sia D, Kilpatrick K (2020) Development and validation of a time and motion guide to assess the costs of prevention and control interventions for nosocomial infections: A Delphi method among experts 15:e0242212https://doi.org/10.1371/journal.pone.0242212

  41. Terreni, M., Taccani, M., & Pregnolato, M. (2021). New antibiotics for multidrug-resistant bacterial strains: Latest research developments and future perspectives. Molecules, 26, 2671.

    Article  CAS  Google Scholar 

  42. Yilmaz, A. (2020). The employment of a conformal polydopamine thin layer reduces the cytotoxicity of silver nanoparticles. Turkish Journal of Zoology, 44, 126–133.

    Article  CAS  Google Scholar 

  43. Zhen, X., Stålsby Lundborg, C., Sun, X., Zhu, N., Gu, S., & Dong, H. (2021). Economic burden of antibiotic resistance in China: A national level estimate for inpatients Antimicrobial Resistance & Infection. Control, 10, 5. https://doi.org/10.1186/s13756-020-00872-w

    Article  Google Scholar 

Download references

Acknowledgements

The authors would like to acknowledge the National Research Centre and Faculty of Science, Al-Azhar University, Cairo, Egypt.

Funding

This work was funded and supported by the Egyptian Academy of Scientific Research and Technology (ASRT) in the frames of the project “Reducing hospital microbial infections through innovative textiles treated with Nanotechnology” (grant number ID6800).

Author information

Authors and Affiliations

  1. Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt

    Amr M. Shehabeldine & Amr H. Hashem

  2. Electron Microscope and Thin Film Department, Physics Research Division, National Research Centre, Dokki, Cairo, 12622, Egypt

    Ahmed R. Wassel

  3. Cellulose and Paper Department, National Research Centre, Dokki, 12622, Cairo, Egypt

    Mohamed Hasanin

Authors
  1. Amr M. Shehabeldine
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Amr H. Hashem
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ahmed R. Wassel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohamed Hasanin
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Amr Shehabeldine: Conceptualization, methodology, formal analysis and investigation, writing—original draft preparation, writing—review and editing, resources, software; Amr Hashem: Conceptualization, methodology, formal analysis and investigation, writing—original draft preparation, writing—review and editing, resources, software; Ahmed Wassel: Methodology, original draft preparation, writing—review and editing; Mohamed Hasanin: Conceptualization, methodology, formal analysis and investigation, writing—original draft preparation, writing—review and editing, resources, software.

Corresponding authors

Correspondence to Amr H. Hashem or Mohamed Hasanin.

Ethics declarations

Ethics Approval

Not applicable.

Consent to Participate

Not applicable.

Consent for Publication

Not applicable.

Conflicts of Interest

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shehabeldine, A.M., Hashem, A.H., Wassel, A.R. et al. Antimicrobial and Antiviral Activities of Durable Cotton Fabrics Treated with Nanocomposite Based on Zinc Oxide Nanoparticles, Acyclovir, Nanochitosan, and Clove Oil. Appl Biochem Biotechnol 194, 783–800 (2022). https://doi.org/10.1007/s12010-021-03649-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-021-03649-y

Keywords

Search

Navigation